Jie Wan, Jian Ding, Xiaoli Zhang, Xinyi Hu, Rui Chen, Su Han
{"title":"靶向代谢组学分析揭示中华绒螯虾感染大鼠的氨基酸代谢轮廓和途径","authors":"Jie Wan, Jian Ding, Xiaoli Zhang, Xinyi Hu, Rui Chen, Su Han","doi":"10.1089/vbz.2023.0059","DOIUrl":null,"url":null,"abstract":"Background: Clonorchiasis remains a serious public health problem. However, the molecular mechanism underlying clonorchiasis remains largely unknown. Amino acid (AA) metabolism plays key roles in protein synthesis and energy sources, and improves immunity in pathological conditions. Therefore, this study aimed to explore the AA profiles of spleen in clonorchiasis and speculate the interaction between the host and parasite. Methods: Here targeted ultrahigh performance liquid chromatography multiple reaction monitoring mass spectrometry was applied to discover the AA profiles in spleen of rats infected with Clonorchis sinensis. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis (KEGG) was performed to characterize the dysregulated metabolic pathways. Results: Pathway analysis revealed that phenylalanine, tyrosine, and tryptophan biosynthesis and β-alanine metabolism were significantly altered in clonorchiasis. There were no significant correlations between 14 significant differential AAs and interleukin (IL)-1β. Although arginine, asparagine, histidine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine were positively correlated with IL-6, IL-10, tumor necrosis factor (TNF)-α as well as aspartate aminotransferase and alanine aminotransferase; β-alanine and 4-hydroxyproline were negatively correlated with IL-6, IL-10, and TNF-α. Conclusion: This study reveals the dysregulation of AA metabolism in clonorchiasis and provides a useful insight of metabolic mechanisms at the molecular level.","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploration of the Amino Acid Metabolic Profiling and Pathway in Clonorchis sinensis-Infected Rats Revealed by the Targeted Metabolomic Analysis.\",\"authors\":\"Jie Wan, Jian Ding, Xiaoli Zhang, Xinyi Hu, Rui Chen, Su Han\",\"doi\":\"10.1089/vbz.2023.0059\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Clonorchiasis remains a serious public health problem. However, the molecular mechanism underlying clonorchiasis remains largely unknown. Amino acid (AA) metabolism plays key roles in protein synthesis and energy sources, and improves immunity in pathological conditions. Therefore, this study aimed to explore the AA profiles of spleen in clonorchiasis and speculate the interaction between the host and parasite. Methods: Here targeted ultrahigh performance liquid chromatography multiple reaction monitoring mass spectrometry was applied to discover the AA profiles in spleen of rats infected with Clonorchis sinensis. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis (KEGG) was performed to characterize the dysregulated metabolic pathways. Results: Pathway analysis revealed that phenylalanine, tyrosine, and tryptophan biosynthesis and β-alanine metabolism were significantly altered in clonorchiasis. There were no significant correlations between 14 significant differential AAs and interleukin (IL)-1β. Although arginine, asparagine, histidine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine were positively correlated with IL-6, IL-10, tumor necrosis factor (TNF)-α as well as aspartate aminotransferase and alanine aminotransferase; β-alanine and 4-hydroxyproline were negatively correlated with IL-6, IL-10, and TNF-α. Conclusion: This study reveals the dysregulation of AA metabolism in clonorchiasis and provides a useful insight of metabolic mechanisms at the molecular level.\",\"PeriodicalId\":23683,\"journal\":{\"name\":\"Vector borne and zoonotic diseases\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-04-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Vector borne and zoonotic diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1089/vbz.2023.0059\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vector borne and zoonotic diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1089/vbz.2023.0059","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
摘要
背景:克隆氏虫病仍然是一个严重的公共卫生问题。然而,克隆氏虫病的分子机制在很大程度上仍然未知。氨基酸(AA)代谢在蛋白质合成和能量来源中起着关键作用,并在病理条件下提高免疫力。因此,本研究旨在探讨克隆氏病患者脾脏的氨基酸谱,并推测宿主与寄生虫之间的相互作用。方法:本研究采用靶向超高效液相色谱-多反应监测质谱法检测了感染中华克隆氏蛔虫的大鼠脾脏中的AA含量。通过京都基因组百科全书途径富集分析(KEGG)来确定代谢途径失调的特征。结果显示通路分析表明,苯丙氨酸、酪氨酸和色氨酸的生物合成以及β-丙氨酸的代谢在克隆氏病中发生了显著改变。14 种明显不同的 AA 与白细胞介素(IL)-1β 之间没有明显的相关性。虽然精氨酸、天冬酰胺、组氨酸、赖氨酸、蛋氨酸、苯丙氨酸、脯氨酸、丝氨酸、苏氨酸、色氨酸、酪氨酸和缬氨酸与 IL-6、IL-10、肿瘤坏死因子(TNF)-α 以及天冬氨酸氨基转移酶和丙氨酸氨基转移酶呈正相关;但 β-丙氨酸和 4-羟脯氨酸与 IL-6、IL-10 和 TNF-α 呈负相关。结论这项研究揭示了克隆氏病中 AA 代谢的失调,为从分子水平了解代谢机制提供了有益的启示。
Exploration of the Amino Acid Metabolic Profiling and Pathway in Clonorchis sinensis-Infected Rats Revealed by the Targeted Metabolomic Analysis.
Background: Clonorchiasis remains a serious public health problem. However, the molecular mechanism underlying clonorchiasis remains largely unknown. Amino acid (AA) metabolism plays key roles in protein synthesis and energy sources, and improves immunity in pathological conditions. Therefore, this study aimed to explore the AA profiles of spleen in clonorchiasis and speculate the interaction between the host and parasite. Methods: Here targeted ultrahigh performance liquid chromatography multiple reaction monitoring mass spectrometry was applied to discover the AA profiles in spleen of rats infected with Clonorchis sinensis. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis (KEGG) was performed to characterize the dysregulated metabolic pathways. Results: Pathway analysis revealed that phenylalanine, tyrosine, and tryptophan biosynthesis and β-alanine metabolism were significantly altered in clonorchiasis. There were no significant correlations between 14 significant differential AAs and interleukin (IL)-1β. Although arginine, asparagine, histidine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine were positively correlated with IL-6, IL-10, tumor necrosis factor (TNF)-α as well as aspartate aminotransferase and alanine aminotransferase; β-alanine and 4-hydroxyproline were negatively correlated with IL-6, IL-10, and TNF-α. Conclusion: This study reveals the dysregulation of AA metabolism in clonorchiasis and provides a useful insight of metabolic mechanisms at the molecular level.
期刊介绍:
Vector-Borne and Zoonotic Diseases is an authoritative, peer-reviewed journal providing basic and applied research on diseases transmitted to humans by invertebrate vectors or non-human vertebrates. The Journal examines geographic, seasonal, and other risk factors that influence the transmission, diagnosis, management, and prevention of this group of infectious diseases, and identifies global trends that have the potential to result in major epidemics.
Vector-Borne and Zoonotic Diseases coverage includes:
-Ecology
-Entomology
-Epidemiology
-Infectious diseases
-Microbiology
-Parasitology
-Pathology
-Public health
-Tropical medicine
-Wildlife biology
-Bacterial, rickettsial, viral, and parasitic zoonoses