用 SEM-EDX 方法分析瓦拉卡(Waraka)和瓦拉(Wala)菠萝籽粉样品的结构和元素

Y. T. Senaweera, B. Prasantha
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摘要

菠萝籽粉的多样化取决于其表型和感官特征。种子粉是淀粉、膳食纤维和矿物质的良好来源。扫描电子显微镜-能量色散 X 射线光谱分析(SEM-EDX)可快速、无损地测定样品中的元素组成,并可随时识别生物材料中的某些元素。因此,本研究的目的是确定瓦拉卡(waraka)和瓦拉(wala)这两个柚子品种的种子粉样品的结构形态特征和矿物质元素。Waraka 和 Wala 的菠萝种子粉样品是用去核干燥种子制备的。使用 SEM-EDX 方法确定了超结构中的元素分布。对两种柚子种子样品的扫描电镜观察显示出不同的结构形态。在瓦卡种子面粉中观察到钟形淀粉颗粒。瓦拉籽粉中的淀粉颗粒呈球形。瓦卡和瓦拉籽粉的平均淀粉颗粒大小分别为 8.00±0.78 μm 和 8.93±0.13 μm。瓦拉籽粉的宏量元素含量相对高于瓦卡籽粉。瓦拉中的宏量元素为:氧 47%、碳 41%、氮 8%、钠 2%、钾 0.7%。瓦拉卡籽粉含有 53% 的氧、41% 的碳和 0.5% 的钾。研究证实,瓦拉卡和瓦拉两个柚子品种的种子面粉的结构形态和元素组成是不同的。
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Structural and Elemental Analysis of Waraka and Wala Jackfruit Seed Flour Samples by SEM-EDX Method
Diversification of jackfruit seed flour depends on its phenotypic and organoleptic characteristics. Seed flour is of good source of starch, dietary fiber, and minerals. Scanning electron microscopy-energy dispersive X-ray spectroscopy analysis (SEM-EDX) provides a quick non-destructive determination of the elemental composition of the sample readily identifying some elements present in the biological materials. Therefore, the objectives of this study were to determine the structural morphological characteristics and mineral elements present in the seed flour samples of two Jackfruit varieties, waraka and wala. Jackfruit seeds flour samples of waraka and wala were prepared by using de-coted dried seeds. The SEM-EDX method was used to identify the element profile within the ultra-structure. SEM observation of the two jackfruit seed samples showed different structure morphology. Bell-shape starch granules were observed in waraka seed flour. Spherical shape starch granules were observed in wala seed flour. The mean starch granule size of the waraka and wala seed flour were 8.00±0.78 μm and 8.93±0.13 μm, respectively. Wala flour showed comparatively higher macro-element content than waraka flour. The macro elements present in wala were 47% oxygen, 41% carbon, 8% nitrogen, 2% sodium and 0.7% potassium. Waraka seed flour contained 53% of oxygen, 41% of carbon, and 0.5% of potassium. Study confirmed that structural morphology and element composition of seed flour are different between two jackfruit varieties, waraka and wala.
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