利用海水评估香蕉假茎回缩机理:具有成本效益的表面预处理方法

IF 20.2 Q1 MATERIALS SCIENCE, PAPER & WOOD Journal of Bioresources and Bioproducts Pub Date : 2024-04-10 DOI:10.1016/j.jobab.2024.04.002
Prince Hotor , Ahmed H. Hassanin , Osbert Akatwijuka , Mohamed A.H. Gepreel , Mitsuo Yamamoto , Yukie Saito , Ahmed Abdel-Mawgood
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引用次数: 0

摘要

几个世纪以来,人们一直采用 Retting 方法从农业废弃物中提取天然纤维,这是一种生物方法,而且成本效益高。香蕉假茎在全球都很丰富,是一种很有前景的提取木质纤维素纤维的农业废弃物。在本研究中,在四种条件下使用海水在室温下预处理长达 35 d 后,从假茎中提取了纤维。分析了翻新过程中的细菌数量以及漆酶和锰过氧化物酶的活性。此外,还对预处理和未处理的提取纤维进行了傅立叶变换红外光谱(FT-IR)和 X 射线衍射(XRD)分析。结果表明,八种细菌分离物中有六种具有降解木质素的能力。在浸提过程的第 14 天,处理(生茎+生海水)和处理(高压灭菌茎+生海水)记录到的最高存活细菌量分别为 9.24 × 102 和 4.46 × 102 CFU。此外,在第二周至第三周,(生茎+生海水)和(高压灭菌茎+生海水)处理的漆酶和锰过氧化物酶活性最高。预处理纤维的傅立叶变换红外光谱显示,在所有浸提条件下,多糖和其他无定形物质的峰值都相对减少。XRD 衍射图显示,在所有海水退火处理条件下,预处理纤维的结晶度指数(CI)都有所增加。不过,在酶解条件下预处理的纤维的结晶度指数甚至在五周之后都有所提高。对所选细菌分离物进行的序列分析表明,它们与 Velezensis 杆菌、Shewanella sp.L8-5、Citrobacter amalonaticus 和 Bacillus subtilis j8 菌株的序列具有同源性。这些发现表明,物理、生物和化学作用共同参与了香蕉假茎的海水腐熟过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Evaluating mechanism of banana pseudo-stem retting using seawater: A cost-effective surface pre-treatment approach

Retting has been employed to extract natural fibers from agricultural wastes as a biological and cost-effective approach for centuries. With its global abundance, banana pseudo-stem is a promising agro-waste for lignocellulosic fiber extraction. In this study, fibers were extracted from the pseudo-stems after being pre-treated under four conditions using seawater at room temperature for up to 35 d Bacterial isolation from the fresh seawater sample and screening for ligninolytic ability were conducted. Bacterial load as well as laccase and manganese peroxidase enzyme activity profile assay during the retting duration were analyzed. Fourier transform infrared (FT-IR) and X-day diffraction (XRD) analyses were also examined for both pre-treated and untreated extracted fibers. The results shows that six out of the eight bacterial isolates had the ability to degrade lignin. The treatments (Raw stem + Raw seawater) and (Autoclaved stem + Raw seawater) recorded the highest viable bacterial load of 9.24 × 102 and 4.46 × 102 CFU, respectively, on the 14th day of the retting process. Additionally, the highest laccase and manganese peroxidase enzymes activity was recorded for (Raw stem + Raw seawater) and (Autoclaved stem + Raw seawater) treatments in the second to the third week. The FT-IR spectra of the pre-treated fibers revealed relative reductions in peaks attributed to polysaccharides and other amorphous substances for all retting conditions. The XRD diffractogram revealed that the crystallinity index (CI) of pre-treated fibers increased in all seawater retting treatment conditions. However, the CI for fibers pre-treated under enzymatic conditions were enhanced even after five weeks. Sequence analysis for selected bacterial isolates showed homology to sequences of Bacillus velezensis, Shewanella sp. L8–5, and Citrobacter amalonaticus and Bacillus subtilis j8 strain. From these findings, it was suggested that physical, biological, and chemical actions were collectively involved in the seawater retting process of banana pseudo-stems.

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来源期刊
Journal of Bioresources and Bioproducts
Journal of Bioresources and Bioproducts Agricultural and Biological Sciences-Forestry
CiteScore
39.30
自引率
0.00%
发文量
38
审稿时长
12 weeks
期刊最新文献
Editorial Board Enhanced biomass densification pretreatment using binary chemicals for efficient lignocellulosic valorization Development of Methylene Bis-Benzotriazolyl Tetramethylbutylphenol-grafted lignin sub-microspheres loaded with TiO2 for sunscreen applications Cavitation as a zero-waste circular economy process to convert citrus processing waste into biopolymers in high demand Selective biomass conversion over novel designed tandem catalyst
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