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引用次数: 0
摘要
经改造的枯草芽孢杆菌可产生可直接传给另一种枯草芽孢杆菌的环状亚基因组。整合到枯草芽孢杆菌基因组中的共轭质粒 pLS20 不仅支持亚基因组的复制，还支持向另一个枯草芽孢杆菌物种的传递。本研究开发的亚基因组系统为枯草杆菌完成巨型 DNA 的合成到传播提供了一个简化的平台。
Integrated conjugal plasmid pLS20 in the Bacillus subtilis genome produced 850-kbp circular subgenomes transmissible to another B. subtilis
Bacillus subtilis was engineered to produce circular subgenomes that are directly transmittable to another B. subtilis. The conjugational plasmid pLS20 integrated into the B. subtilis genome supported not only subgenome replication but also transmission to another B. subtilis species. The subgenome system developed in this study completes a streamlined platform from the synthesis to the transmission of giant DNA by B. subtilis.
期刊介绍：
Genes to Cells provides an international forum for the publication of papers describing important aspects of molecular and cellular biology. The journal aims to present papers that provide conceptual advance in the relevant field. Particular emphasis will be placed on work aimed at understanding the basic mechanisms underlying biological events.
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