基于磁珠阵列的荧光免疫分析平台,用于临床血液样本中癌症生物标记物的可靠分析

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Microfluidics and Nanofluidics Pub Date : 2024-04-29 DOI:10.1007/s10404-024-02728-w
Jieung Oh, Ho-Seong Han, Hyundoo Hwang, Joo H. Kang
{"title":"基于磁珠阵列的荧光免疫分析平台,用于临床血液样本中癌症生物标记物的可靠分析","authors":"Jieung Oh,&nbsp;Ho-Seong Han,&nbsp;Hyundoo Hwang,&nbsp;Joo H. Kang","doi":"10.1007/s10404-024-02728-w","DOIUrl":null,"url":null,"abstract":"<div><p>The reliable conjugation of antibodies to a solid matrix is crucial for robust immunoassays in microfluidic devices. Various magnetic particles (MPs) have been employed due to their high surface-to-volume ratios and ease of magnetic manipulation, providing a reliable surface for antibody immobilization. However, achieving uniform positioning of MPs across the channel surface has been challenging due to inadequate magnetic forces or magnetic field uniformity. Here, we present the utilization of Halbach magnetic arrays to enable consistent deployment of MPs on the microfluidic surface, thereby facilitating robust immunoassay capabilities. Using finite element method magnetics (FEMM) simulations, we predicted that incorporating Halbach magnetic arrays beneath the microfluidic channels would create more uniform and augmented magnetic flux density gradients over the surface. Subsequently, we applied this platform to assess cancer biomarkers in patients’ blood plasma and achieved statistically reliable results, comparable to those obtained using an FDA-approved device. We detected three cancer biomarkers, including prostate-specific antigen (PSA), alpha-fetoprotein (AFP), and carcinoembryonic antigen (CEA). The limit of detection (LOD) of three biomarkers were &lt; 1 ng/mL, ranging from 0.38–0.95 ng mL<sup>−1</sup>. This platform provided within-run, between-run, and between-day precisions for the three cancer biomarkers, ranging from 0.37–9.87%. This advancement holds significant promise for improving the accuracy and performance of immunoassays in various microfluidic diagnostic applications.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 5","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A magnetic bead array-based fluorescence immunoassay platform for robust cancer biomarker analysis in clinical blood samples\",\"authors\":\"Jieung Oh,&nbsp;Ho-Seong Han,&nbsp;Hyundoo Hwang,&nbsp;Joo H. Kang\",\"doi\":\"10.1007/s10404-024-02728-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The reliable conjugation of antibodies to a solid matrix is crucial for robust immunoassays in microfluidic devices. Various magnetic particles (MPs) have been employed due to their high surface-to-volume ratios and ease of magnetic manipulation, providing a reliable surface for antibody immobilization. However, achieving uniform positioning of MPs across the channel surface has been challenging due to inadequate magnetic forces or magnetic field uniformity. Here, we present the utilization of Halbach magnetic arrays to enable consistent deployment of MPs on the microfluidic surface, thereby facilitating robust immunoassay capabilities. Using finite element method magnetics (FEMM) simulations, we predicted that incorporating Halbach magnetic arrays beneath the microfluidic channels would create more uniform and augmented magnetic flux density gradients over the surface. Subsequently, we applied this platform to assess cancer biomarkers in patients’ blood plasma and achieved statistically reliable results, comparable to those obtained using an FDA-approved device. We detected three cancer biomarkers, including prostate-specific antigen (PSA), alpha-fetoprotein (AFP), and carcinoembryonic antigen (CEA). The limit of detection (LOD) of three biomarkers were &lt; 1 ng/mL, ranging from 0.38–0.95 ng mL<sup>−1</sup>. This platform provided within-run, between-run, and between-day precisions for the three cancer biomarkers, ranging from 0.37–9.87%. This advancement holds significant promise for improving the accuracy and performance of immunoassays in various microfluidic diagnostic applications.</p></div>\",\"PeriodicalId\":706,\"journal\":{\"name\":\"Microfluidics and Nanofluidics\",\"volume\":\"28 5\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-04-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microfluidics and Nanofluidics\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10404-024-02728-w\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INSTRUMENTS & INSTRUMENTATION\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microfluidics and Nanofluidics","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10404-024-02728-w","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INSTRUMENTS & INSTRUMENTATION","Score":null,"Total":0}
引用次数: 0

摘要

抗体与固体基质的可靠结合对于在微流控装置中进行稳健的免疫测定至关重要。各种磁性颗粒(MPs)因其高表面体积比和易于磁性操作而被广泛使用,为抗体固定提供了可靠的表面。然而,由于磁力不足或磁场不均匀,要在通道表面实现 MPs 的均匀定位一直是个挑战。在此,我们介绍利用哈尔巴赫磁阵列在微流体表面实现 MPs 的一致部署,从而促进强大的免疫测定功能。利用有限元法磁学(FEMM)模拟,我们预测在微流控通道下方安装哈尔巴赫磁阵列可在表面形成更均匀的增强磁通密度梯度。随后,我们将这一平台用于评估患者血浆中的癌症生物标志物,并取得了统计上可靠的结果,与使用美国食品及药物管理局批准的设备所取得的结果相当。我们检测了三种癌症生物标记物,包括前列腺特异性抗原(PSA)、甲胎蛋白(AFP)和癌胚抗原(CEA)。三种生物标记物的检测限(LOD)均为1纳克/毫升,范围为0.38-0.95纳克毫升-1。该平台为三种癌症生物标记物提供了运行内、运行间和日间精确度,精确度范围为 0.37-9.87%。这一进步为提高各种微流控诊断应用中免疫测定的准确性和性能带来了巨大希望。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
A magnetic bead array-based fluorescence immunoassay platform for robust cancer biomarker analysis in clinical blood samples

The reliable conjugation of antibodies to a solid matrix is crucial for robust immunoassays in microfluidic devices. Various magnetic particles (MPs) have been employed due to their high surface-to-volume ratios and ease of magnetic manipulation, providing a reliable surface for antibody immobilization. However, achieving uniform positioning of MPs across the channel surface has been challenging due to inadequate magnetic forces or magnetic field uniformity. Here, we present the utilization of Halbach magnetic arrays to enable consistent deployment of MPs on the microfluidic surface, thereby facilitating robust immunoassay capabilities. Using finite element method magnetics (FEMM) simulations, we predicted that incorporating Halbach magnetic arrays beneath the microfluidic channels would create more uniform and augmented magnetic flux density gradients over the surface. Subsequently, we applied this platform to assess cancer biomarkers in patients’ blood plasma and achieved statistically reliable results, comparable to those obtained using an FDA-approved device. We detected three cancer biomarkers, including prostate-specific antigen (PSA), alpha-fetoprotein (AFP), and carcinoembryonic antigen (CEA). The limit of detection (LOD) of three biomarkers were < 1 ng/mL, ranging from 0.38–0.95 ng mL−1. This platform provided within-run, between-run, and between-day precisions for the three cancer biomarkers, ranging from 0.37–9.87%. This advancement holds significant promise for improving the accuracy and performance of immunoassays in various microfluidic diagnostic applications.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
期刊最新文献
High throughput cell mechanotyping of cell response to cytoskeletal modulations using a microfluidic cell deformation system High-throughput separation of microalgae on a runway-shaped channel with ordered semicircular micro-obstacles Future electrodes for sepsis detection: digital microfluidic biosensors from plant waste Visualizing conformance control mechanisms in high-temperature reservoirs: a microfluidic analysis of Pickering emulsified gel systems Exploring fluid flow in microchannels with branching and variable constrictions
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1