N-乙酰半胱氨酸对肥胖成人内脏脂肪组织中 UCP1 和甲状腺功能相关因子表达的影响:一项随机双盲临床试验

IF 3.5 3区 医学 Q1 Medicine Genes and Nutrition Pub Date : 2024-05-03 DOI:10.1186/s12263-024-00744-7
Mohammad Hassan Sohouli, Ghazaleh Eslamian, Seyed Hossein Ardehali, Seyed Ahmad Raeissadat, Ghazaleh Shimi, Katayoun Pourvali, Hamid Zand
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引用次数: 0

摘要

有证据表明,肥胖受多种因素影响,包括各种激素,如甲状腺激素和人体新陈代谢率。减肥饮食和常用药物等实际解决方案似乎可以影响这些潜在的疾病。在本研究中,我们调查了这些常用药物之一--N-乙酰半胱氨酸(NAC)--对成人肥胖症患者体内 UCP1 的表达和甲状腺功能相关因素的影响。本次调查以随机临床试验(RCT)的形式进行,包括 43 名可能接受减肥手术的成年肥胖症患者。这些人被随机分为两组:服用 600 毫克 NAC(22 人)或安慰剂(21 人),为期 8 周。利用减肥手术中的内脏脂肪组织来研究 UCP1 的基因表达和甲状腺功能。聚合酶链反应(PCR)一式两份,按照提供的说明检测 UCP1、DIO2、DIO3、THRα 和 β 以及 18s RNA(作为内部对照),以研究相应基因的表达。我们的研究结果表明,与安慰剂相比,8 周后,NAC 会导致与甲状腺功能和新陈代谢相关的基因之一 DIO3 基因的表达显著下降。然而,在其他相关基因方面,尽管 UCP1、DIO2 和 THRα 的表达量有所增加,而 THRβ 的表达量则有所减少,但在统计学上却没有发现明显的影响(尽管 UCP1、DIO2 和 THRα 的表达量有所增加,而 THRβ 的表达量则有所减少)。此外,在调整了可能的混杂因素后,也没有观察到对人体测量因素和血清甲状腺激素水平的显著影响。本研究结果表明,与安慰剂相比,NAC 能在 8 周内有效降低内脏脂肪组织中 DIO3 基因的表达。
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Effects of N-acetylcysteine on the expressions of UCP1 and factors related to thyroid function in visceral adipose tissue of obese adults: a randomized, double-blind clinical trial
Evidences have shown that obesity is influenced by various factors, including various hormones such as thyroid hormones and the body’s metabolism rate. It seems that practical solutions such as weight loss diets and common drugs can affect these potential disorders. In this study, we investigate one of these common drugs, N-Acetylcysteine (NAC), on expressions of UCP1 and factors related to thyroid function in adults with obesity. The current investigation was carried out as a randomized clinical trial (RCT) including 43 adults with obesity who were potential candidates for bariatric surgery. These individuals were randomly divided into two groups: 600 mg of NAC (n = 22) or placebo (n = 21) for a duration of 8 weeks. Visceral adipose tissue was utilized in the context of bariatric surgery to investigate the gene expression of UCP1 and thyroid function. Polymerase chain reaction (PCR) was performed in duplicate for UCP1, DIO2, DIO3, THRα and β, and 18s RNA (as an internal control) using the provided instructions to investigate the expression of the respective genes. Our findings revealed that after 8 weeks compared to placebo, NAC caused a significant decrease in the expression of the DIO3 gene as one of the genes related to thyroid function and metabolism. However, regarding other related genes, no statistically significant was found (despite the increase in UCP1, DIO2, and THRα expression and decrease in THRβ expression). In addition, after adjustment of possible confounders, no significant effect was observed on anthropometric factors and serum levels of thyroid hormones. The results of this study indicate that, following an 8-week period, NAC effectively decreases the expression of the DIO3 gene in the visceral fat tissue, in comparison to the placebo.
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来源期刊
Genes and Nutrition
Genes and Nutrition Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
7.90
自引率
0.00%
发文量
14
审稿时长
13 weeks
期刊介绍: This journal examines the relationship between genetics and nutrition, with the ultimate goal of improving human health. It publishes original research articles and review articles on preclinical research data coming largely from animal, cell culture and other experimental models as well as critical evaluations of human experimental data to help deliver products with medically proven use.
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