{"title":"从植物材料中绿色分离挥发性物质和酚类化合物的综合两步超临界流体萃取法","authors":"Veronika Pilařová , Kateřina Plachká , Diana Herbsová , Štefan Kosturko , Frantisek Svec , Lucie Nováková","doi":"10.1039/d4gc00371c","DOIUrl":null,"url":null,"abstract":"<div><p>Extraction of compounds with different physicochemical properties from a complex matrix usually involves several individual steps and requires large volumes of organic solvents. In this pioneering study, we propose a comprehensive two-step supercritical fluid extraction using carbon dioxide, ethanol, and water. This novel approach allows the extraction of non-polar and polar analytes within one run in two consecutive steps. Indeed, the first step with a dominant amount of CO<sub>2</sub> with only 2% cosolvent allowed the selective extraction of non-polar volatile terpenes only in 20 min. The conditions were then automatically switched. Increasing the cosolvent volume in the extraction solvent up to 44% (v/v) resulted in the extraction of more polar compounds, including flavonoids and phenolic acids, in 60 min. Importantly, switching the supercritical fluid extraction (SFE) conditions does not require any manual intervention but results in two separate fractions containing target compounds with distinctly different physicochemical properties. The novel method was verified in terms of repeatability, accuracy, precision, and greenness. Two-step SFE was applied to seven plant species differing in volatile terpenes and phenolic profiles. The results proved that this concept is suitable for the analysis of complex plant samples. In addition, it enables a reduction in the toxic solvents consumption, extraction time, and manual intervention required for traditional extraction approaches when isolating different groups of metabolites.</p></div>","PeriodicalId":78,"journal":{"name":"Green Chemistry","volume":null,"pages":null},"PeriodicalIF":9.3000,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comprehensive two-step supercritical fluid extraction for green isolation of volatiles and phenolic compounds from plant material†\",\"authors\":\"Veronika Pilařová , Kateřina Plachká , Diana Herbsová , Štefan Kosturko , Frantisek Svec , Lucie Nováková\",\"doi\":\"10.1039/d4gc00371c\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Extraction of compounds with different physicochemical properties from a complex matrix usually involves several individual steps and requires large volumes of organic solvents. In this pioneering study, we propose a comprehensive two-step supercritical fluid extraction using carbon dioxide, ethanol, and water. This novel approach allows the extraction of non-polar and polar analytes within one run in two consecutive steps. Indeed, the first step with a dominant amount of CO<sub>2</sub> with only 2% cosolvent allowed the selective extraction of non-polar volatile terpenes only in 20 min. The conditions were then automatically switched. Increasing the cosolvent volume in the extraction solvent up to 44% (v/v) resulted in the extraction of more polar compounds, including flavonoids and phenolic acids, in 60 min. Importantly, switching the supercritical fluid extraction (SFE) conditions does not require any manual intervention but results in two separate fractions containing target compounds with distinctly different physicochemical properties. The novel method was verified in terms of repeatability, accuracy, precision, and greenness. Two-step SFE was applied to seven plant species differing in volatile terpenes and phenolic profiles. The results proved that this concept is suitable for the analysis of complex plant samples. In addition, it enables a reduction in the toxic solvents consumption, extraction time, and manual intervention required for traditional extraction approaches when isolating different groups of metabolites.</p></div>\",\"PeriodicalId\":78,\"journal\":{\"name\":\"Green Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":9.3000,\"publicationDate\":\"2024-06-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Green Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/org/science/article/pii/S1463926224004990\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Green Chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/org/science/article/pii/S1463926224004990","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Comprehensive two-step supercritical fluid extraction for green isolation of volatiles and phenolic compounds from plant material†
Extraction of compounds with different physicochemical properties from a complex matrix usually involves several individual steps and requires large volumes of organic solvents. In this pioneering study, we propose a comprehensive two-step supercritical fluid extraction using carbon dioxide, ethanol, and water. This novel approach allows the extraction of non-polar and polar analytes within one run in two consecutive steps. Indeed, the first step with a dominant amount of CO2 with only 2% cosolvent allowed the selective extraction of non-polar volatile terpenes only in 20 min. The conditions were then automatically switched. Increasing the cosolvent volume in the extraction solvent up to 44% (v/v) resulted in the extraction of more polar compounds, including flavonoids and phenolic acids, in 60 min. Importantly, switching the supercritical fluid extraction (SFE) conditions does not require any manual intervention but results in two separate fractions containing target compounds with distinctly different physicochemical properties. The novel method was verified in terms of repeatability, accuracy, precision, and greenness. Two-step SFE was applied to seven plant species differing in volatile terpenes and phenolic profiles. The results proved that this concept is suitable for the analysis of complex plant samples. In addition, it enables a reduction in the toxic solvents consumption, extraction time, and manual intervention required for traditional extraction approaches when isolating different groups of metabolites.
期刊介绍:
Green Chemistry is a journal that provides a unique forum for the publication of innovative research on the development of alternative green and sustainable technologies. The scope of Green Chemistry is based on the definition proposed by Anastas and Warner (Green Chemistry: Theory and Practice, P T Anastas and J C Warner, Oxford University Press, Oxford, 1998), which defines green chemistry as the utilisation of a set of principles that reduces or eliminates the use or generation of hazardous substances in the design, manufacture and application of chemical products. Green Chemistry aims to reduce the environmental impact of the chemical enterprise by developing a technology base that is inherently non-toxic to living things and the environment. The journal welcomes submissions on all aspects of research relating to this endeavor and publishes original and significant cutting-edge research that is likely to be of wide general appeal. For a work to be published, it must present a significant advance in green chemistry, including a comparison with existing methods and a demonstration of advantages over those methods.