健康奶牛外耳道的霉菌生物群

IF 2.7 3区医学 Q3 INFECTIOUS DISEASES Medical mycology Pub Date : 2024-04-30 DOI:10.1093/mmy/myae049

Leyna Díaz, Gemma Castellá, M Rosa Bragulat, Andreu Paytuví-Gallart, Walter Sanseverino, F Javier Cabañes

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引用次数: 0

摘要

马拉色菌酵母属于多种温血动物的正常皮肤微生物群。然而，人们对它们在牛体内的重要性仍然知之甚少。本研究通过细胞学、培养、PCR 和新一代测序对 20 头健康荷斯坦奶牛外耳道的霉菌生物群进行了评估。通过细胞学和 PCR 检测，15 头奶牛体内存在马拉色菌。元基因组分析表明，Ascomycota 是主要的菌门，但 M. pachydermatis 是主要菌种。马拉色菌系统型 131 的检出率较低。在样本中也没有检测到马氏菌（M. nana）和马氏菌（M. equina）。

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Mycobiome of the external ear canal of healthy cows

Malassezia yeasts belong to the normal skin microbiota of a wide range of warm-blooded animals. However, their significance in cattle is still poorly understood. In the present study, the mycobiota of the external ear canal of 20 healthy dairy Holstein cows was assessed by cytology, culture, PCR, and next-generation sequencing. The presence of Malassezia was detected in 15 cows by cytology and PCR. The metagenomic analysis revealed that Ascomycota was the predominant phylum but M. pachydermatis the main species. The Malassezia phylotype 131 was detected in low abundance. Nor M. nana nor M. equina were detected in the samples.

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来源期刊

Medical mycology 医学-兽医学

CiteScore

5.70

自引率

3.40%

发文量

632

审稿时长

12 months

期刊介绍： Medical Mycology is a peer-reviewed international journal that focuses on original and innovative basic and applied studies, as well as learned reviews on all aspects of medical, veterinary and environmental mycology as related to disease. The objective is to present the highest quality scientific reports from throughout the world on divergent topics. These topics include the phylogeny of fungal pathogens, epidemiology and public health mycology themes, new approaches in the diagnosis and treatment of mycoses including clinical trials and guidelines, pharmacology and antifungal susceptibilities, changes in taxonomy, description of new or unusual fungi associated with human or animal disease, immunology of fungal infections, vaccinology for prevention of fungal infections, pathogenesis and virulence, and the molecular biology of pathogenic fungi in vitro and in vivo, including genomics, transcriptomics, metabolomics, and proteomics. Case reports are no longer accepted. In addition, studies of natural products showing inhibitory activity against pathogenic fungi are not accepted without chemical characterization and identification of the compounds responsible for the inhibitory activity.