从罗伯逊易位中提取滋养层细胞用于产前诊断

IF 1.9 4区医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE Alternative therapies in health and medicine Pub Date : 2025-01-01

Zhen Xu, Sheng Li, Long He

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引用次数: 0

摘要

目的提供罗伯逊（Rob）易位携带者胎儿的遗传信息，并探讨从宫颈管收集的绒毛外滋养层细胞（EVTs）在产前诊断中的应用价值：方法：宫颈滋养层细胞检索和分离（TRIC）是一种无创分离均质滋养层细胞的方法。本研究从 20 名妊娠 5-7 周的孕妇宫颈中收集了 EVT 细胞。随后，对这些滋养层细胞进行了STR分析和荧光原位杂交（FISH）：结果：1 例（P5）未能采集到滋养层细胞，4 例的 STR 分析显示母体细胞受到污染，6 例为正常/平衡染色体，9 例伴有不平衡染色体。这 15 个病例的结果与绒毛 FISH 检查或培养羊膜细胞的细胞遗传学分析结果一致：结论：从宫颈收集胎儿滋养层细胞为产前诊断提供了一种可行的方法。Rob 易位同源染色体可被视为人类 44 条染色体分化的一种潜在手段。

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Retrieval of Trophoblast Cells from The Robertsonian Translocations for Prenatal Diagnosis.

Objective: To provide genetic information about the fetuses from carriers of Robertsonian (Rob) translocation and to explore the application value of extravillous trophoblasts (EVTs) cells collected from the cervical canal for prenatal diagnosis.

Method: Trophoblast retrieval and isolation from the cervix (TRIC) is an approach that non-invasively isolates homogeneous trophoblast cells. In this study, the EVT cells were collected from the cervix of 20 pregnant women between 5-7 weeks gestation. Thereafter, STR analysis and fluorescence in situ hybridization (FISH) were performed on these trophoblast cells.

Results: In 1 case (P5), we failed to collect the trophoblast cells, STR analysis showed maternal cell contamination in 4 cases, 6 cases were normal/balanced chromosome, and 9 cases were associated with unbalanced chromosome. The results of these 15 cases were consistent with those of villi FISH examination or cytogenetic analysis of cultured amniocytes.

Conclusion: The collection of fetal trophoblast cells from the cervix provides a feasible approach for prenatal diagnosis. Rob translocation homozygosity could be seen as a potential means of speciation in humans with 44 chromosomes.

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来源期刊

Alternative therapies in health and medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

0.90

自引率

0.00%

发文量

219

期刊介绍： Launched in 1995, Alternative Therapies in Health and Medicine has a mission to promote the art and science of integrative medicine and a responsibility to improve public health. We strive to maintain the highest standards of ethical medical journalism independent of special interests that is timely, accurate, and a pleasure to read. We publish original, peer-reviewed scientific articles that provide health care providers with continuing education to promote health, prevent illness, and treat disease. Alternative Therapies in Health and Medicine was the first journal in this field to be indexed in the National Library of Medicine. In 2006, 2007, and 2008, ATHM had the highest impact factor ranking of any independently published peer-reviewed CAM journal in the United States—meaning that its research articles were cited more frequently than any other journal’s in the field. Alternative Therapies in Health and Medicine does not endorse any particular system or method but promotes the evaluation and appropriate use of all effective therapeutic approaches. Each issue contains a variety of disciplined inquiry methods, from case reports to original scientific research to systematic reviews. The editors encourage the integration of evidence-based emerging therapies with conventional medical practices by licensed health care providers in a way that promotes a comprehensive approach to health care that is focused on wellness, prevention, and healing. Alternative Therapies in Health and Medicine hopes to inform all licensed health care practitioners about developments in fields other than their own and to foster an ongoing debate about the scientific, clinical, historical, legal, political, and cultural issues that affect all of health care.