{"title":"[龈沟液中的短链脂肪酸与Ⅲ期或Ⅳ期牙周炎的关系]。","authors":"Yuru Hu, Juan Liu, Wenjing Li, Yibing Zhao, Qiqiang Li, Ruifang Lu, Huanxin Meng","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To analyze the concentration of formic acid, propionic acid and butyric acid in gingival crevicular fluid (GCF) of patients with stages Ⅲ and Ⅳ periodontitis, and their relationship with periodontitis.</p><p><strong>Methods: </strong>The study enrolled 37 systemically healthy patients with periodontitis and 19 healthy controls who visited Department of Periodontology, Peking University School and Hospital of Stomatology from February 2008 to May 2011. Their GCFs were collected from the mesial-buccal site of one molar or incisor in each quadrant. Periodontal clinical parameters, including plaque index(PLI), probing depth(PD), bleeding index(BI), and attachment loss(AL). Concentrations of formic acid, propionic acid and butyric acid in the supernatant of the GCFs were analyzed by high-performance capillary electrophoresis (HPCE). The prediction ability of formic acid, propionic acid and butyric acid with the risk of periodontitis and the differences between grade B and grade C periodontitis were analyzed.</p><p><strong>Results: </strong>In this study, 32 patients with stage Ⅲ and 5 patients with stage Ⅳ were enrolled, including 9 patients with grade B and 28 patients with grade C. Clinical periodontal variables in the patients with periodontitis were significantly higher than those in the control group (<i>P</i><0.001). Formic acid was significantly lower in periodontitis than that in the control group [5.37 (3.39, 8.49) mmol/L <i>vs.</i> 12.29 (8.35, 16.57) mmol/L, <i>P</i><0.001]. Propionic acid and butyric acid in periodontitis were significantly higher than those in the control group: Propionic acid, 10.23 (4.28, 14.90) mmol/L <i>vs.</i> 2.71 (0.00, 4.25) mmol/L, <i>P</i> < 0.001; butyric acid, 2.63 (0.47, 3.81) mmol/L <i>vs.</i> 0.00 (0.00, 0.24) mmol/L, <i>P</i><0.001. There was no significant difference in formic acid, propionic acid and butyric acid concentrations between grade B and grade C periodontitis (<i>P</i>>0.05). Propionic acid and butyric acid in the deep pocket were significantly higher than in the shallow pocket, while the concentration of formic acid decreased with the increase of PD. Propionic acid (<i>OR</i>=1.51, 95%<i>CI</i>: 1.29-1.75) and butyric acid (<i>OR</i>=3.72, 95%<i>CI</i>: 1.93-7.17) were risk factors for periodontitis, while formic acid (<i>OR</i>=0.87, 95%<i>CI</i>: 0.81-0.93) might be a protective factor for periodontitis. Propionic acid (AUC=0.852, 95%<i>CI</i>: 0.805-0.900), butyric acid (AUC=0.889, 95%<i>CI</i>: 0.841-0.937), f (formic acid, AUC=0.844, 95%<i>CI</i>: 0.793-0.895) demonstrated a good predictive capacity for the risk of periodontitis.</p><p><strong>Conclusion: </strong>The concentration of formic acid decrease in the GCF of periodontitis patients, which is a protective factor for periodontitis, its reciprocal have good predictive capacity. However, propionic acid and butyric acid increase, which are risk factors for periodontitis and have good predictive capacity. The concentration of formic acid, propionic acid, and butyric acid vary with probing depth, but there is no significant difference between grade B and grade C periodontitis.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报(医学版)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11004952/pdf/","citationCount":"0","resultStr":"{\"title\":\"[Relationship between short-chain fatty acids in the gingival crevicular fluid and periodontitis of stage Ⅲ or Ⅳ].\",\"authors\":\"Yuru Hu, Juan Liu, Wenjing Li, Yibing Zhao, Qiqiang Li, Ruifang Lu, Huanxin Meng\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To analyze the concentration of formic acid, propionic acid and butyric acid in gingival crevicular fluid (GCF) of patients with stages Ⅲ and Ⅳ periodontitis, and their relationship with periodontitis.</p><p><strong>Methods: </strong>The study enrolled 37 systemically healthy patients with periodontitis and 19 healthy controls who visited Department of Periodontology, Peking University School and Hospital of Stomatology from February 2008 to May 2011. Their GCFs were collected from the mesial-buccal site of one molar or incisor in each quadrant. Periodontal clinical parameters, including plaque index(PLI), probing depth(PD), bleeding index(BI), and attachment loss(AL). Concentrations of formic acid, propionic acid and butyric acid in the supernatant of the GCFs were analyzed by high-performance capillary electrophoresis (HPCE). The prediction ability of formic acid, propionic acid and butyric acid with the risk of periodontitis and the differences between grade B and grade C periodontitis were analyzed.</p><p><strong>Results: </strong>In this study, 32 patients with stage Ⅲ and 5 patients with stage Ⅳ were enrolled, including 9 patients with grade B and 28 patients with grade C. Clinical periodontal variables in the patients with periodontitis were significantly higher than those in the control group (<i>P</i><0.001). Formic acid was significantly lower in periodontitis than that in the control group [5.37 (3.39, 8.49) mmol/L <i>vs.</i> 12.29 (8.35, 16.57) mmol/L, <i>P</i><0.001]. Propionic acid and butyric acid in periodontitis were significantly higher than those in the control group: Propionic acid, 10.23 (4.28, 14.90) mmol/L <i>vs.</i> 2.71 (0.00, 4.25) mmol/L, <i>P</i> < 0.001; butyric acid, 2.63 (0.47, 3.81) mmol/L <i>vs.</i> 0.00 (0.00, 0.24) mmol/L, <i>P</i><0.001. There was no significant difference in formic acid, propionic acid and butyric acid concentrations between grade B and grade C periodontitis (<i>P</i>>0.05). Propionic acid and butyric acid in the deep pocket were significantly higher than in the shallow pocket, while the concentration of formic acid decreased with the increase of PD. Propionic acid (<i>OR</i>=1.51, 95%<i>CI</i>: 1.29-1.75) and butyric acid (<i>OR</i>=3.72, 95%<i>CI</i>: 1.93-7.17) were risk factors for periodontitis, while formic acid (<i>OR</i>=0.87, 95%<i>CI</i>: 0.81-0.93) might be a protective factor for periodontitis. Propionic acid (AUC=0.852, 95%<i>CI</i>: 0.805-0.900), butyric acid (AUC=0.889, 95%<i>CI</i>: 0.841-0.937), f (formic acid, AUC=0.844, 95%<i>CI</i>: 0.793-0.895) demonstrated a good predictive capacity for the risk of periodontitis.</p><p><strong>Conclusion: </strong>The concentration of formic acid decrease in the GCF of periodontitis patients, which is a protective factor for periodontitis, its reciprocal have good predictive capacity. However, propionic acid and butyric acid increase, which are risk factors for periodontitis and have good predictive capacity. The concentration of formic acid, propionic acid, and butyric acid vary with probing depth, but there is no significant difference between grade B and grade C periodontitis.</p>\",\"PeriodicalId\":8790,\"journal\":{\"name\":\"北京大学学报(医学版)\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11004952/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"北京大学学报(医学版)\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"北京大学学报(医学版)","FirstCategoryId":"3","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
摘要
目的分析Ⅲ期和Ⅳ期牙周炎患者牙龈细缝液(GCF)中甲酸、丙酸和丁酸的浓度及其与牙周炎的关系:研究对象为2008年2月至2011年5月在北京大学口腔医学院附属北京大学口腔医院牙周科就诊的37名全身健康的牙周炎患者和19名健康对照者。他们的 GCF 都是从每个象限的一颗磨牙或切牙的颊面部位采集的。牙周临床参数包括牙菌斑指数(PLI)、探诊深度(PD)、出血指数(BI)和附着丧失指数(AL)。采用高性能毛细管电泳(HPCE)分析了GCF上清液中甲酸、丙酸和丁酸的浓度。分析了甲酸、丙酸和丁酸对牙周炎风险的预测能力以及B级和C级牙周炎之间的差异:本研究共纳入 32 例Ⅲ期患者和 5 例Ⅳ期患者,其中 B 级患者 9 例,C 级患者 28 例。牙周炎患者的临床牙周变量明显高于对照组(Pvs.12.29(8.35,16.57)mmol/L,Pvs.2.71(0.00,4.25)mmol/L,P<0.001;丁酸,2.63(0.47,3.81)mmol/L vs. 0.00(0.00,0.24)mmol/L,PP>0.05)。深袋中的丙酸和丁酸明显高于浅袋,而甲酸的浓度随着 PD 的增加而降低。丙酸(OR=1.51,95%CI:1.29-1.75)和丁酸(OR=3.72,95%CI:1.93-7.17)是牙周炎的危险因素,而甲酸(OR=0.87,95%CI:0.81-0.93)可能是牙周炎的保护因素。丙酸(AUC=0.852,95%CI:0.805-0.900)、丁酸(AUC=0.889,95%CI:0.841-0.937)、甲酸(AUC=0.844,95%CI:0.793-0.895)对牙周炎的风险具有良好的预测能力:结论:牙周炎患者 GCF 中甲酸浓度的下降是牙周炎的保护因素,其倒数具有良好的预测能力。然而,丙酸和丁酸浓度升高,这是牙周炎的危险因素,具有良好的预测能力。甲酸、丙酸和丁酸的浓度随探诊深度的变化而变化,但在 B 级和 C 级牙周炎之间没有显著差异。
[Relationship between short-chain fatty acids in the gingival crevicular fluid and periodontitis of stage Ⅲ or Ⅳ].
Objective: To analyze the concentration of formic acid, propionic acid and butyric acid in gingival crevicular fluid (GCF) of patients with stages Ⅲ and Ⅳ periodontitis, and their relationship with periodontitis.
Methods: The study enrolled 37 systemically healthy patients with periodontitis and 19 healthy controls who visited Department of Periodontology, Peking University School and Hospital of Stomatology from February 2008 to May 2011. Their GCFs were collected from the mesial-buccal site of one molar or incisor in each quadrant. Periodontal clinical parameters, including plaque index(PLI), probing depth(PD), bleeding index(BI), and attachment loss(AL). Concentrations of formic acid, propionic acid and butyric acid in the supernatant of the GCFs were analyzed by high-performance capillary electrophoresis (HPCE). The prediction ability of formic acid, propionic acid and butyric acid with the risk of periodontitis and the differences between grade B and grade C periodontitis were analyzed.
Results: In this study, 32 patients with stage Ⅲ and 5 patients with stage Ⅳ were enrolled, including 9 patients with grade B and 28 patients with grade C. Clinical periodontal variables in the patients with periodontitis were significantly higher than those in the control group (P<0.001). Formic acid was significantly lower in periodontitis than that in the control group [5.37 (3.39, 8.49) mmol/L vs. 12.29 (8.35, 16.57) mmol/L, P<0.001]. Propionic acid and butyric acid in periodontitis were significantly higher than those in the control group: Propionic acid, 10.23 (4.28, 14.90) mmol/L vs. 2.71 (0.00, 4.25) mmol/L, P < 0.001; butyric acid, 2.63 (0.47, 3.81) mmol/L vs. 0.00 (0.00, 0.24) mmol/L, P<0.001. There was no significant difference in formic acid, propionic acid and butyric acid concentrations between grade B and grade C periodontitis (P>0.05). Propionic acid and butyric acid in the deep pocket were significantly higher than in the shallow pocket, while the concentration of formic acid decreased with the increase of PD. Propionic acid (OR=1.51, 95%CI: 1.29-1.75) and butyric acid (OR=3.72, 95%CI: 1.93-7.17) were risk factors for periodontitis, while formic acid (OR=0.87, 95%CI: 0.81-0.93) might be a protective factor for periodontitis. Propionic acid (AUC=0.852, 95%CI: 0.805-0.900), butyric acid (AUC=0.889, 95%CI: 0.841-0.937), f (formic acid, AUC=0.844, 95%CI: 0.793-0.895) demonstrated a good predictive capacity for the risk of periodontitis.
Conclusion: The concentration of formic acid decrease in the GCF of periodontitis patients, which is a protective factor for periodontitis, its reciprocal have good predictive capacity. However, propionic acid and butyric acid increase, which are risk factors for periodontitis and have good predictive capacity. The concentration of formic acid, propionic acid, and butyric acid vary with probing depth, but there is no significant difference between grade B and grade C periodontitis.