Prisha Balgovind, Gerald Murray, Dorothy A Machalek, Suzanne M Garland, Francesco Azzato, Jee A Hinaut, Jennifer Danielewski, Monica Molano, Gholamreza Haqshenas
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引用次数: 0
摘要
基于 PCR 的诊断测定的效率会受到 DNA 模板质量的影响,而肛门样本由于存在粪便污染物,可能会特别成问题。在这里,我们比较了 Quick-DNA 病毒试剂盒(Zymo,加州 Zymo Research 公司)和 MagNA Pure 96 DNA 与病毒 NA 小容量试剂盒(MP96,罗氏公司)在使用 Seegene Anyplex II HPV28 检测试剂盒(Anyplex28,Seegene 公司)检测肛门样本时的效果。共有94份使用MP96和Zymo试剂盒提取的肛门样本通过Anyplex28进行了检测,Anyplex28可检测高危型人类乳头瘤病毒(HR-HPV,A组)和低危型(LR-HPV,B组)HPV类型。在检测 HR-HPV 类型(A 组)时,86 份 MP96 样品(91.5%)和 84 份 Zymo 样品(89.4%)被认为是可评估的。两种方法的总体一致性为 87/94(92.6%,95% CI:85.3-97.0),Kappa 值为 0.678(0.5-0.9)。在 87 份可评估样本中,50 份(57.5%)一致,34 份(39.1%)部分一致,10 份(11.5%)不一致。总之,使用这两种方法中的任何一种提取的 DNA,Anyplex28 都能产生相似的 HPV 基因分型结果。
Seegene Anyplex II assays detect HPV consistently using DNA extracts from different extraction methods.
The efficiency of PCR-based diagnostic assays can be impacted by the quality of DNA template, and anal samples can be particularly problematic due to the presence of faecal contaminants. Here, we compared the Quick-DNA Viral Kit (Zymo, Zymo Research, CA) and MagNA Pure 96 DNA and Viral NA Small Volume Kit (MP96, Roche) for use of the Seegene Anyplex II HPV28 assay (Anyplex28, Seegene) with anal samples. A total of 94 anal samples extracted using the MP96 and Zymo kits were tested via the Anyplex28, which detects high-risk human papillomavirus (HR-HPV, Panel A) and low-risk (LR-HPV, Panel B) HPV types. Testing the HR-HPV types (Panel A), 86 (91.5%) MP96 and 84 (89.4%) Zymo samples were deemed assessable. Overall agreement between the two methods was 87/94 (92.6%, 95% CI: 85.3-97.0) with the Kappa value of 0.678 (0.5-0.9). Of the 87 assessable samples, 50 (57.5%) were concordant, 34 (39.1%) partially concordant, and 10 (11.5%)discordant. In conclusion, the Anyplex28 produces comparable HPV genotyping results when using DNA extracts from either of these two methods.