南非队列中唇鳞状细胞癌高危 HPV 评估。

IF 3.2 Q2 PATHOLOGY Head & Neck Pathology Pub Date : 2024-05-06 DOI:10.1007/s12105-024-01639-0
Sharon N Harbor, Johann W Schneider, Nadine Solomons, Micheline Sanderson, Amir H Afrogheh
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引用次数: 0

摘要

背景:采用目前公认的HPV检测方法,确定HR-HPV在一系列南非患者唇部SCC中的流行率,并确定HPV相关唇部SCC的临床和组织形态学特征:采用反转录定量实时聚合酶链反应(RT-qPCR)进一步评估 HPV-16 和 HPV-18 mRNA 转录本的表达情况,以确认转录活跃的 HPV。结果:22%(n = 11)的 SCC 中 p16 呈阳性,4%(n = 2)呈阳性。一个 p16 阳性病例显示 HPV-16 DNA 和 HPV-16 E6/E7 mRNA 转录本均呈阳性(HPV 感染率为 2%)。该 HPV 阳性病例为非角化型,发生在一名 80 岁的女性身上。两例p16阳性病例均为HR-HPV DNA阳性,mRNA PCR阴性:我们对唇部 SCC 的研究结果表明,HR-HPV 在该部位 SCC 的发病机制中可能作用不大。由于 p16 的 ppv 值较低,因此不足以确定唇部 SCC 是否感染了 HR-HPV。p16 和 DNA PCR 的结合似乎与转录活跃病毒的存在有关。HPV E6/E7 mRNA 检测是鉴别 HR-HPV 的黄金标准。由于技术和资金方面的限制,mRNA 检测在撒哈拉以南非洲地区并不普及;不过,该检测在 p16 明确的唇部 SCC 中似乎具有重要价值。
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An Evaluation of High-Risk HPV in Squamous Cell Carcinomas of the Lip in a South African Cohort.

Background: To determine the prevalence of HR-HPV in a series of lip SCC from South African patients, using currently accepted HPV-testing methodologies and to define the clinical and histomorphologic features of HPV-associated lip SCC.

Methods: Fifty SCC of lip and 50 control cases were tested for HR-HPV using p16 and HR-HPV DNA PCR. p16-equivocal/positive and HPV DNA PCR-positive SCC were further evaluated for the expression of HPV-16 and HPV-18 mRNA transcripts using reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) to confirm transcriptionally active HPV.

Results: p16 was positive in 22% (n = 11) and equivocal in 4% (n = 2) of the SCC. One p16-positive case showed positivity for both HPV-16 DNA and HPV-16 E6/E7 mRNA transcripts (HPV prevalence rate of 2%). The HPV-positive case was non-keratinizing and occurred in an 80-year-old female. The two p16-equivocal cases were HR-HPV DNA positive and mRNA PCR negative. p16 was found to have a positive predictive value of 9%.

Conclusion: Findings from our cohort of lip SCC suggest that HR-HPV may have an insignificant role in the pathogenesis of SCC at this site. Due to its low ppv, p16 is insufficient to establish HR-HPV infection in SCC of the lip. The combination of p16 and DNA PCR appears to correlate with the presence of transcriptionally active virus. HPV E6/E7 mRNA detection is the gold standard for identifying HR-HPV. mRNA testing is not widely available in sub-Saharan Africa due to technical and financial constraints; however, the test appears to be of great value in p16-equivocal lip SCC.

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来源期刊
CiteScore
5.70
自引率
9.50%
发文量
99
期刊介绍: Head & Neck Pathology presents scholarly papers, reviews and symposia that cover the spectrum of human surgical pathology within the anatomic zones of the oral cavity, sinonasal tract, larynx, hypopharynx, salivary gland, ear and temporal bone, and neck. The journal publishes rapid developments in new diagnostic criteria, intraoperative consultation, immunohistochemical studies, molecular techniques, genetic analyses, diagnostic aids, experimental pathology, cytology, radiographic imaging, and application of uniform terminology to allow practitioners to continue to maintain and expand their knowledge in the subspecialty of head and neck pathology. Coverage of practical application to daily clinical practice is supported with proceedings and symposia from international societies and academies devoted to this field. Single-blind peer review The journal follows a single-blind review procedure, where the reviewers are aware of the names and affiliations of the authors, but the reviewer reports provided to authors are anonymous. Single-blind peer review is the traditional model of peer review that many reviewers are comfortable with, and it facilitates a dispassionate critique of a manuscript.
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