蔓越橘微繁殖培养基的有机添加剂。

Q4 Biochemistry, Genetics and Molecular Biology Methods in molecular biology Pub Date : 2024-01-01 DOI:10.1007/978-1-0716-3782-1_11
Marcelina Krupa-Małkiewicz, Ireneusz Ochmian, Monika Figiel-Kroczyńska
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引用次数: 0

摘要

在离体培养条件下,使用正确的培养基成分和酸碱度可以使越橘植物生长得最好。在离体培养的初始阶段,通常可以使用细胞分裂素(主要是玉米素)的组合。在培养基中添加有机物可以替代高丛蓝莓微繁殖中使用的昂贵的天然细胞分裂素。本章介绍了使用硅 Hydroplus™ Actisil(Si)、椰子水(CW)和不同 pH 值(5.0、5.5 和 6.0)作为胁迫因子的实验。添加 200 mg dm-3 硅溶液和 15%的椰子水能强烈刺激高丛蓝莓植物的离体生长。此外,硅溶液还能缓解用于蓝莓微繁殖的培养基 pH 值升高所带来的负面影响。蓝莓外植体在 pH 值为 5 时获得最大的无性发育。
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Organic Supplementation of Vaccinium corymbosum Micropropagation Media.

The best Vaccinium corymbosum plant growth under in vitro conditions can be achieved by using the right composition and pH of the medium. For the initial phase of in vitro culture, a combination of cytokinins-mostly zeatin-can usually be used. Organic supplementation of the medium enables the use of a replacement for the expensive natural cytokinin used in micropropagation of highbush blueberry. This chapter describes the experiments with silicon Hydroplus™ Actisil (Si), coconut water (CW), and different pH (5.0; 5.5, and 6.0) as a stress factor. The addition of 200 mg dm-3 silicon solution and 15% coconut water strongly stimulated highbush blueberry plant growth in vitro. Moreover, silicon solution benefits the negative effects of higher pH of the medium used for micropropagation of V. corymbosum. Maximum vegetative development of blueberry explants was obtained at pH 5.

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来源期刊
Methods in molecular biology
Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
2.00
自引率
0.00%
发文量
3536
期刊介绍: For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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