果蝇科(昆虫纲:双翅目)中的水平转移和伽利略转座子的广泛存在。

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-03-29 eCollection Date: 2024-01-01 DOI:10.1590/1678-4685-GMB-2023-0143
Henrique R M Antoniolli, Sebastián Pita, Maríndia Deprá, Vera L S Valente
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引用次数: 0

摘要

伽利略(Galileo)是一种臭名昭著的转座子,它通过异位重组与嗡嗡果蝇的倒位有关。虽然该转座子在果蝇中广泛存在,但人们对其在果蝇科其他品系中的情况知之甚少。在此,我们估算并重建了果蝇科两个亚科中各属的典型伽利略转座子的丰度及其在果蝇科基因组中的进化历史。在这些基因组中屏蔽了该转座子的序列,并使用 BLASTn 恢复了它们的转座酶序列。利用系统发生学分析重建了它们的进化史,并与宿主基因组的进化史进行了比较。几乎在所有 163 个物种中都发现了伽利略,但只有 37 个物种拥有几乎完整的转座酶序列。在其余的物种中,伽利略被发现是高度片段化的。来自相关物种的拷贝是聚集在一起的,但在黑腹果蝇群和montium果蝇群之间,以及后者与Lordiphosa果蝇属之间发现了水平转移事件。在果蝇的 virilis 群和 willistoni 群中发现的序列相似性被认为是品系分选的结果。因此,伽利略的进化主要表现为垂直传播和长期失活,主要是通过删除开放阅读框。后者有可能导致该转座子的拷贝成为微型倒转重复转座元件。
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Horizontal transfer and the widespread presence of Galileo transposons in Drosophilidae (Insecta: Diptera).

Galileo is a transposon notoriously involved with inversions in Drosophila buzzatii by ectopic recombination. Although widespread in Drosophila, little is known about this transposon in other lineages of Drosophilidae. Here, the abundance of the canonical Galileo and its evolutionary history in Drosophilidae genomes was estimated and reconstructed across genera within its two subfamilies. Sequences of this transposon were masked in these genomes and their transposase sequences were recovered using BLASTn. Phylogenetic analyses were employed to reconstruct their evolutionary history and compare it to that of host genomes. Galileo was found in nearly all 163 species, however, only 37 harbored nearly complete transposase sequences. In the remaining, Galileo was found highly fragmented. Copies from related species were clustered, however horizontal transfer events were detected between the melanogaster and montium groups of Drosophila, and between the latter and the Lordiphosa genus. The similarity of sequences found in the virilis and willistoni groups of Drosophila was found to be a consequence of lineage sorting. Therefore, the evolution of Galileo is primarily marked by vertical transmission and long-term inactivation, mainly through the deletion of open reading frames. The latter has the potential to lead copies of this transposon to become miniature inverted-repeat transposable elements.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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