Matthew Waas, Amanda Khoo, Pirashaanthy Tharmapalan, Curtis W McCloskey, Meinusha Govindarajan, Bowen Zhang, Shahbaz Khan, Paul D Waterhouse, Rama Khokha, Thomas Kislinger
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Droplet-based proteomics reveals CD36 as a marker for progenitors in mammary basal epithelium.
Deep proteomic profiling of rare cell populations has been constrained by sample input requirements. Here, we present DROPPS (droplet-based one-pot preparation for proteomic samples), an accessible low-input platform that generates high-fidelity proteomic profiles of 100-2,500 cells. By applying DROPPS within the mammary epithelium, we elucidated the connection between mitochondrial activity and clonogenicity, identifying CD36 as a marker of progenitor capacity in the basal cell compartment. We anticipate that DROPPS will accelerate biology-driven proteomic research for a multitude of rare cell populations.
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