{"title":"比较全血和血浆在监测巨细胞病毒和 Epstein-Barr 病毒方面的作用。","authors":"Yuto Fukuda, Yuka Torii, Ken-Ichi Iwata, Kazunori Haruta, Makoto Yamaguchi, Takako Suzuki, Atsushi Narita, Hideki Muramatsu, Yasuhiro Ogura, Yoshiyuki Takahashi, Yoshinori Ito, Jun-Ichi Kawada","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Monitoring Epstein-Barr virus (EBV) and cytomegalovirus (CMV) infection after transplantation is recommended to enable preemptive therapy. However, the most suitable sample type remains unclear. Patients who underwent hematopoietic stem cell or liver transplantation were included in this study. Viral loads in sequential whole-blood and plasma samples were retrospectively analyzed. EBV DNA was detected more frequently in whole blood (55%) than in plasma (18%). The detection rate of CMV DNA was similar between the two sample types. The correlation of viral loads between the two sample types were 0.515 and 0.688 for EBV and CMV, respectively. Among paired samples in which EBV DNA was detected in whole blood, the plasma EBV detection rate was significantly higher in patients who underwent hematopoietic stem cell transplantation than in those who underwent liver transplantation. The viral DNA load in whole blood and plasma showed similar trends. The EBV detection rate was higher in whole blood, and a high correlation was observed between CMV DNA loads and whole blood and plasma. These results indicate that whole blood is more sensitive for monitoring both EBV and CMV, whereas plasma is a potential alternative sample for monitoring CMV.</p>","PeriodicalId":54723,"journal":{"name":"New Microbiologica","volume":"47 1","pages":"52-59"},"PeriodicalIF":1.5000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of Whole Blood and Plasma for Monitoring Cytomegalovirus and Epstein-Barr Virus.\",\"authors\":\"Yuto Fukuda, Yuka Torii, Ken-Ichi Iwata, Kazunori Haruta, Makoto Yamaguchi, Takako Suzuki, Atsushi Narita, Hideki Muramatsu, Yasuhiro Ogura, Yoshiyuki Takahashi, Yoshinori Ito, Jun-Ichi Kawada\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Monitoring Epstein-Barr virus (EBV) and cytomegalovirus (CMV) infection after transplantation is recommended to enable preemptive therapy. However, the most suitable sample type remains unclear. Patients who underwent hematopoietic stem cell or liver transplantation were included in this study. Viral loads in sequential whole-blood and plasma samples were retrospectively analyzed. EBV DNA was detected more frequently in whole blood (55%) than in plasma (18%). The detection rate of CMV DNA was similar between the two sample types. The correlation of viral loads between the two sample types were 0.515 and 0.688 for EBV and CMV, respectively. Among paired samples in which EBV DNA was detected in whole blood, the plasma EBV detection rate was significantly higher in patients who underwent hematopoietic stem cell transplantation than in those who underwent liver transplantation. The viral DNA load in whole blood and plasma showed similar trends. The EBV detection rate was higher in whole blood, and a high correlation was observed between CMV DNA loads and whole blood and plasma. These results indicate that whole blood is more sensitive for monitoring both EBV and CMV, whereas plasma is a potential alternative sample for monitoring CMV.</p>\",\"PeriodicalId\":54723,\"journal\":{\"name\":\"New Microbiologica\",\"volume\":\"47 1\",\"pages\":\"52-59\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"New Microbiologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Microbiologica","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
建议在移植后监测爱泼斯坦-巴氏病毒（EBV）和巨细胞病毒（CMV）感染，以便进行预防性治疗。然而，最合适的样本类型仍不明确。本研究纳入了接受造血干细胞或肝移植的患者。对连续全血和血浆样本中的病毒载量进行了回顾性分析。EBV DNA在全血中的检出率（55%）高于血浆（18%）。两种样本中 CMV DNA 的检出率相似。两种样本中 EBV 和 CMV 病毒载量的相关性分别为 0.515 和 0.688。在全血中检测到 EBV DNA 的配对样本中，造血干细胞移植患者的血浆 EBV 检测率明显高于肝移植患者。全血和血浆中的病毒 DNA 负载显示出相似的趋势。全血中的 EBV 检出率更高，CMV DNA 负载与全血和血浆之间存在高度相关性。这些结果表明，全血对监测 EBV 和 CMV 更为敏感，而血浆则是监测 CMV 的潜在替代样本。
Comparison of Whole Blood and Plasma for Monitoring Cytomegalovirus and Epstein-Barr Virus.
Monitoring Epstein-Barr virus (EBV) and cytomegalovirus (CMV) infection after transplantation is recommended to enable preemptive therapy. However, the most suitable sample type remains unclear. Patients who underwent hematopoietic stem cell or liver transplantation were included in this study. Viral loads in sequential whole-blood and plasma samples were retrospectively analyzed. EBV DNA was detected more frequently in whole blood (55%) than in plasma (18%). The detection rate of CMV DNA was similar between the two sample types. The correlation of viral loads between the two sample types were 0.515 and 0.688 for EBV and CMV, respectively. Among paired samples in which EBV DNA was detected in whole blood, the plasma EBV detection rate was significantly higher in patients who underwent hematopoietic stem cell transplantation than in those who underwent liver transplantation. The viral DNA load in whole blood and plasma showed similar trends. The EBV detection rate was higher in whole blood, and a high correlation was observed between CMV DNA loads and whole blood and plasma. These results indicate that whole blood is more sensitive for monitoring both EBV and CMV, whereas plasma is a potential alternative sample for monitoring CMV.
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The publication, diffusion and furtherance of research and study on all aspects of basic and clinical Microbiology and related fields are the chief aims of the journal.
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