NADPH 氧化酶 4 对小鼠干眼症的作用

IF 1.9 4区 医学 Q2 OPHTHALMOLOGY Journal of Ocular Pharmacology and Therapeutics Pub Date : 2024-09-01 Epub Date: 2024-04-26 DOI:10.1089/jop.2024.0002
Mian Guo, Taixiang Liu, Yuan Miao, Xiaoli Pan, Bo Liu
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引用次数: 0

摘要

研究目的本研究旨在探讨 NADPH 氧化酶 4(NOX4)介导的炎症对 concanavalin A(ConA)诱导的小鼠干眼症(DES)的影响。研究方法将36只小鼠随机分为对照组、模型组、无负荷对照组和NOX4干扰组。向无负荷对照组和 NOX4 干扰组小鼠双眼泪腺注射腺病毒(10 μL)。注射腺病毒四天后,给对照组小鼠注射磷酸盐缓冲盐水,给其他组小鼠泪腺注射 ConA(200 μg),建立 DES 模型。泪液分泌率通过酚红线试验进行估计。利萨明绿眼药水染色用于评估结膜损伤。通过苏木精-伊红(HE)染色和扫描电子显微镜(SEM)观察角膜表面。用过期酸-希夫染色法观察结膜上皮细胞和鹅口疮细胞的形态和数量。免疫组化法检测了NOX4、NOD样受体热蛋白结构域相关蛋白3(NLRP3)、白细胞介素-1β(IL-1β)和粘蛋白5亚型AC(MUC5AC)的表达。结果与对照组相比,模型组的泪液分泌明显减少,显微图像评分上升。HE 染色和 SEM 显示模型组角膜和结膜受损。模型组的 NOX4、NLRP3 和 IL-1β 蛋白表达上调,但 MUC5AC 蛋白表达下调。干扰 NOX4 后,所有这些指标都发生了逆转。结论简谐蛋白A诱导的DES的病理过程似乎与NOX4有关。
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Role of NADPH Oxidase 4 on Dry Eye Syndrome in Mice.

Objective: This study aims to investigate the effect of NADPH oxidase 4 (NOX4)-mediated inflammation on concanavalin A (ConA)-induced dry eye syndrome (DES) in mice. Methods: Thirty-six mice were randomly divided into Control, Model, no-load Control, and NOX4 interference group. Adenovirus was injected (10 μL) into the lacrimal glands of both eyes of mice in no-load Control group and NOX4 interference group. Four days after adenovirus injection, the Control group was injected with phosphate-buffered saline, and the other groups were injected with ConA (200 μg) in the lacrimal glands of mice to establish DES models. The tear secretion rate was estimated by phenol red thread test. Lissamine green eye staining was used to evaluate conjunctival damage. The corneal surface was observed by hematoxylin-eosin (HE) staining and scanning electron microscopy (SEM). The morphology and quantity of conjunctival epithelial cells and goblet cells were observed by Periodic acid-Schiff staining. The expression of NOX4, NOD-like receptor thermal protein domain-associated protein 3 (NLRP3), interleukin-1β (IL-1β), and mucin 5 subtype AC (MUC5AC) was detected by immunohistochemistry. Results: Compared with the Control group, the Model group showed a significant decrease in tear secretion and an upregulation in microscopic image score. The HE staining and SEM showed corneal and conjunctiva damage in the Model group. The protein expression of NOX4, NLRP3, and IL-1β was upregulated, but MUC5AC was downregulated in the Model group. After interfering with NOX4, all these indicators were reversed. Conclusion: The pathological process of concanavalin A-induced DES appears to be related to NOX4.

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来源期刊
CiteScore
4.60
自引率
4.30%
发文量
72
审稿时长
1 months
期刊介绍: Journal of Ocular Pharmacology and Therapeutics is the only peer-reviewed journal that combines the fields of ophthalmology and pharmacology to enable optimal treatment and prevention of ocular diseases and disorders. The Journal delivers the latest discoveries in the pharmacokinetics and pharmacodynamics of therapeutics for the treatment of ophthalmic disorders. Journal of Ocular Pharmacology and Therapeutics coverage includes: Glaucoma Cataracts Retinal degeneration Ocular infection, trauma, and toxicology Ocular drug delivery and biotransformation Ocular pharmacotherapy/clinical trials Ocular inflammatory and immune disorders Gene and cell-based therapies Ocular metabolic disorders Ocular ischemia and blood flow Proliferative disorders of the eye Eyes on Drug Discovery - written by Gary D. Novack, PhD, featuring the latest updates on drug and device pipeline developments as well as policy/regulatory changes by the FDA.
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