用于评估 HAM/TSP 患者临床疗效的 HTLV-1 病毒载量绝对 RT-qPCR 开发。

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2023-10-01 DOI:10.61186/rbmb.12.3.393
Nazila Ariaee, Shadi Abbasnia, Faeze Sabet, Ali Mirhossein, Sanaz Ahmadi Ghezeldasht, Mehdi Moshfegh, Reza Boostani, Seyed Abdolrahim Rezaee
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引用次数: 0

摘要

背景:HTLV-1前病毒载量作为HTLV-1相关性脊髓病/热带痉挛性截瘫(HAM/TSP)的预后生物标志物的意义一直存在争议。本研究旨在评估HTLV-1前病毒载量(PVL)对HAM/TSP患者临床结局的影响:方法:开发了一种绝对定量的 HTLV-1 PVL RT-qPCR TaqMan 方法,其灵敏度和特异性均为 100%。然后,从2005年至2018年,对90名符合条件的新诊断HAM/TSP患者的HTLV-1 PVL进行了人口统计学、临床症状及其与HTLV-1-PVL的关联性评估:结果:设计的 RT-qPCR 质量控制显示灵敏度和特异性均为 100%。58.9%的 HAM/TSP 患者出现下肢痉挛,17.8%的 HAM/TSP 患者出现泌尿系统症状。使用这种设计的 RT-qPCR,HTLV-1-PVL 对痉挛和括约肌障碍有很大影响(p=0.05)。多变量逻辑检验显示,只有开始出现下肢无力和震颤才与 PVL 有关(OR:2.78。95%CI(0.99-1.02),P=0.05)。尿失禁在这些患者中很普遍,但未发现与HTLV-1前病毒载量(PVL)有关:结论:为测量 HTLV-1 病毒载量(PVL)而开发的绝对 RT-qPCR 结果可靠。尽管这些患者中尿失禁的发病率很高,但未观察到与 PVL 有任何关联。因此,HTLV-1病毒载量似乎与HAM/TSP患者出现痉挛特别相关。
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HTLV-1 Proviral Load Absolute RT-qPCR Development for Assessing on Clinical Outcomes in HAM/TSP Patients.

Background: The significance of HTLV-1 proviral load as a prognostic biomarker in HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) has been a subject of controversy. This study aims to assess the impact of HTLV-1 proviral load (PVL) on the clinical outcome in patients with HAM/TSP.

Methods: An absolute quantitative HTLV-1 PVL RT-qPCR, TaqMan method was developed with 100% sensitivity and specificity. Then, from 2005-2018, the HTLV-1 PVL of 90 eligible newly diagnosed HAM/TSP patients were assessed for demographic, clinical symptoms and their associations with HTLV-1-PVL.

Results: The quality control of the designed RT-qPCR showed a sensitivity and specificity of 100%. Spasticity in lower limbs in 58.9% and urinary symptoms in 17.8% of HAM/TSPs were observed. Using this designed RT-qPCR, the HTLV-1-PVL strongly affected spasticity and sphincter disturbance (p=0.05). The multivariate logistic test showed that only the beginning of lower limb weakness along with tremor was associated with PVL (OR: 2.78. 95% CI (0.99-1.02) and p=0.05). Urinary incontinence was prevalent among these patients; however, no association was identified with the HTLV-1 proviral load (PVL).

Conclusions: The absolute RT-qPCR developed for measuring HTLV-1 proviral load (PVL) demonstrated reliable results. Despite a high prevalence of urinary incontinence in these patients, no association was observed with the PVL. Consequently, it appears that HTLV-1 proviral load is specifically associated with developing spasticity in HAM/TSP.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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