瑞士无症状水痘病例数量与废水中检测到的水痘病毒 DNA 之间的关系:一项观察性监测研究。

IF 2.1 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Swiss medical weekly Pub Date : 2024-03-07 DOI:10.57187/s.3706
Claudia Bagutti, Monica Alt Hug, Philippe Heim, Evelyn Ilg Hampe, Philipp Hübner, Timothy R Julian, Katrin N Koch, Kerstin Grosheintz, Melanie Kraus, Carla Schaubhut, Rahel Tarnutzer, Eva Würfel, Simon Fuchs, Sarah Tschudin-Sutter
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引用次数: 0

摘要

研究目的:COVID-19 大流行使人们注意到基于废水的流行病学的益处,尤其是在病例数被低报的情况下。低报可能是水痘的一个问题,因为生理原因和耻辱感可能会阻碍患者接受检测。因此,我们旨在评估废水监测对监测中欧某城市废水中天花病毒 DNA 的有效性及其与官方病例数的关联:方法:我们于 2022 年 7 月 1 日至 8 月 28 日期间在瑞士巴塞尔集水区采集了废水样本,并通过实时定量 PCR 测定了其中所含的 mpox 病毒基因组拷贝数。采用逻辑回归分析确定废水中检测到 mpox 病毒 DNA 的几率,分为可检测到和不可检测到两种情况。曼-惠特尼 U 检验用于确定水痘病毒检测呈阳性的样本与官方报告的病例和患者记录的症状阶段之间的关联:结果:39 份废水样本中有 15 份检测到麻风腮病毒 DNA。阳性废水样本的数量与有症状病例的数量相关(几率比 [OR] = 2.18,95% 置信区间 (CI) = 1.38-3.43,p = 0.001)。有症状病例的数量在废水结果为阳性和阴性的天数之间存在显著差异(中位数分别为 11 天和 8 天,p = 0.0024):结论:即使官方报告的病例数很少(0-3 个新报告的麻风病例对应 6-12 个有症状的病人),废水中也能检测到麻风病毒 DNA。废水中的可检测性与集水区内有症状患者的数量有显著关联。这些发现说明了基于废水的监测系统在评估新发和流行性传染病流行情况时的价值。
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Association between the number of symptomatic mpox cases and the detection of mpox virus DNA in wastewater in Switzerland: an observational surveillance study.

Aim of the study: The COVID-19 pandemic has drawn attention to the benefit of wastewater-based epidemiology, particularly when case numbers are underreported. Underreporting may be an issue with mpox, where biological reasons and stigma may prevent patients from getting tested. Therefore, we aimed to assess the validity of wastewater surveillance for monitoring mpox virus DNA in wastewater of a Central European city and its association with official case numbers.

Methods: Wastewater samples were collected between 1 July and 28 August 2022 in the catchment area of Basel, Switzerland, and the number of mpox virus genome copies they contained was determined by real-time quantitative PCR. Logistic regression analyses were used to determine the odds of detectability of mpox virus DNA in wastewater, categorised as detectable or undetectable. Mann-Whitney U tests were used to determine associations between samples that tested positive for the mpox virus and officially reported cases and patients' recorded symptomatic phases.

Results: Mpox virus DNA was detected in 15 of 39 wastewater samples. The number of positive wastewater samples was associated with the number of symptomatic cases (odds ratio [OR] = 2.18, 95% confidence interval (CI) = 1.38-3.43, p = 0.001). The number of symptomatic cases differed significantly between days with positive versus negative wastewater results (median = 11 and 8, respectively, p = 0.0024).

Conclusion: Mpox virus DNA was detectable in wastewater, even when officially reported case numbers were low (0-3 newly reported mpox cases corresponding to 6-12 symptomatic patients). Detectability in wastewater was significantly associated with the number of symptomatic patients within the catchment area. These findings illustrate the value of wastewater-based surveillance systems when assessing the prevalence of emerging and circulating infectious diseases.

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来源期刊
Swiss medical weekly
Swiss medical weekly 医学-医学:内科
CiteScore
5.00
自引率
0.00%
发文量
0
审稿时长
3-8 weeks
期刊介绍: The Swiss Medical Weekly accepts for consideration original and review articles from all fields of medicine. The quality of SMW publications is guaranteed by a consistent policy of rigorous single-blind peer review. All editorial decisions are made by research-active academics.
期刊最新文献
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