人类乳头瘤病毒相关口腔上皮增生症:诊断的实用方法》(Human Papillomavirus-Associated Oral Epithelial Dysplasia: A Practical Approach to Make the Diagnosis)。

IF 1.6 Q2 MEDICINE, GENERAL & INTERNAL Iranian Journal of Medical Sciences Pub Date : 2024-03-01 DOI:10.30476/IJMS.2023.96202.2897
Kiarash Parchami, Samira Derakhshan, Hana Saffar, Pouyan Aminishakib, Ahmad Reza Shamshiri, Samaneh Afshar
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引用次数: 0

摘要

背景:在恶性口腔上皮病变中发现了高危人乳头瘤病毒(HPV)基因型,HPV感染被认为是引发头颈部鳞状细胞癌(SCC)的危险因素。本研究提出了一种检测 HPV 相关口腔上皮发育不良(HAOED)中 HPV 的实用方法:方法:采用二元分级系统检查了54例口腔上皮发育不良标本,其中27例被诊断为高级别发育不良,27例被诊断为低级别发育不良。为了评估病例是否感染了人乳头瘤病毒,研究人员对标本进行了免疫组化(IHC)检查,以检测 p16 蛋白,然后对所有阳性病例进行了染色质原位杂交(CISH)检测。对 CISH 阳性标本进行染色质免疫沉淀聚合酶链反应(ChIP-PCR),以评估结果。这项横断面研究于 2020 年在德黑兰医科大学进行。研究人员使用 SPSS 软件 22.0 版进行了卡方检验或费雪精确检验,以检验变量之间的关系(统计学显著水平):p16 蛋白的表达与上皮发育不良的严重程度无关(低度病例为 81.5%,高度病例为 59.2%)(P=0.16)。此外,根据 CISH 检测结果,9.25% 的标本呈阳性(P>0.99),在进行 ChIP-PCR 研究的 9 例病例中,2 例(22.2%)显示 HPV-16 阳性,1 例(11.1%)显示 HPV-51 阳性:关于 HAOED,我们在此提出了一种基于互补算法的分步组合方法,使用不同的诊断方法,包括 p16 蛋白 IHC、CISH 和 ChIP-PCR。
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Human Papillomavirus-Associated Oral Epithelial Dysplasia: A Practical Approach to Make the Diagnosis.

Background: High-risk Human Papillomavirus (HPV) genotypes are found in malignant oral epithelial lesions, and HPV infection is proposed as a risk factor for initiating Squamous cell carcinoma (SCC) in the head and neck region. This study suggests a practical approach to detect HPV in HPV-associated oral epithelial dysplasia (HAOED).

Methods: Fifty-four oral epithelial dysplasia specimens were examined, comprising twenty-seven cases diagnosed with high-grade dysplasia and twenty-seven cases diagnosed with low-grade dysplasia using a binary grading system. To assess the cases for HPV, the specimens were examined for p16 protein using an immunohistochemical (IHC) study, and then, the Chromatin In Situ Hybridization (CISH) test was performed for all positive cases. Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) was performed on CISH-positive specimens to assess the outcome. This cross-sectional study was conducted in 2020 at Tehran University of Medical Science. SPSS software version 22.0 was used to perform the Chi square or Fisher's exact test to examine the relationship between variables (statistically significant level P<0.05).

Results: The expression of p16 protein was not associated with the severity of epithelial dysplasia (81.5% in low-grade and 59.2% in high-grade cases) (P=0.16). Moreover, according to the CISH test result, 9.25% of all specimens were positive (P>0.99), and in the nine cases, undergone the ChIP-PCR study, two cases (22.2%) showed positivity for HPV-16, while one case (11.1%) demonstrated positivity for HPV-51.

Conclusion: Regarding HAOED, here, we proposed a step-by-step combination approach using different diagnostic methods, including IHC for p16 protein, CISH, and ChIP-PCR based on a complementary algorithm.

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来源期刊
Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
3.20
自引率
0.00%
发文量
84
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Medical Sciences (IJMS) is an international quarterly biomedical publication, which is sponsored by Shiraz University of Medical Sciences. The IJMS intends to provide a scientific medium of com­muni­cation for researchers throughout the globe. The journal welcomes original clinical articles as well as clinically oriented basic science re­search experiences on prevalent diseases in the region and analysis of various regional problems.
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