选定反应监测在精确筛选测试中的适用性。

IF 3.8 3区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS Expert Review of Proteomics Pub Date : 2024-05-01 Epub Date: 2024-05-08 DOI:10.1080/14789450.2024.2350975
Ahrum Son, Woojin Kim, Wonseok Lee, Jongham Park, Hyunsoo Kim
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引用次数: 0

摘要

导言:长期以来,通过筛查检验主动识别疾病一直被认为是预防症状发作的一种手段。然而,这种筛查工作充满了并发症,如诊断不准确、程序风险和检查期间病人的不安。如果同时进行多种疾病的筛查,这些问题就会更加严重。选择反应监测(SRM)具有独特的优势,可对数百种分析物进行高通量定量,且干扰极少:我们的研究认为,基于 SRM 的检测方法传统上是为单病种生物标志物分析而量身定制的,但可以重新用于多病种筛选。这种创新方法有可能大大减轻医疗系统和患者的时间、人力和成本需求。尽管如此,在方法学上仍有许多障碍需要克服。这些障碍包括检测低丰度蛋白质的困难,以及由于不同疾病谱中的单个蛋白质具有多种功能而导致模型过拟合的风险--这些问题在检测灵敏度受限的血液检测中尤为突出。随着我们向前迈进,样品制备、在线提取、通量和自动化方面的技术进步有望改善这些局限性:质谱技术与临床实验室的整合即将成熟,它将成为提供高灵敏度、可重现性和精确诊断结果的宝贵资产。
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Applicability of selected reaction monitoring for precise screening tests.

Introduction: The proactive identification of diseases through screening tests has long been endorsed as a means to preempt symptomatic onset. However, such screening endeavors are fraught with complications, such as diagnostic inaccuracies, procedural risks, and patient unease during examinations. These challenges are amplified when screenings for multiple diseases are administered concurrently. Selected Reaction Monitoring (SRM) offers a unique advantage, allowing for the high-throughput quantification of hundreds of analytes with minimal interferences.

Areas covered: Our research posits that SRM-based assays, traditionally tailored for single-disease biomarker profiling, can be repurposed for multi-disease screening. This innovative approach has the potential to substantially alleviate time, labor, and cost demands on healthcare systems and patients alike. Nonetheless, there are formidable methodological hurdles to overcome. These include difficulties in detecting low-abundance proteins and the risk of model overfitting due to the multiple functionalities of single proteins across different disease spectrums - issues especially pertinent in blood-based assays where detection sensitivity is constrained. As we move forward, technological strides in sample preparation, online extraction, throughput, and automation are expected to ameliorate these limitations.

Expert opinion: The maturation of mass spectrometry's integration into clinical laboratories appears imminent, positioning it as an invaluable asset for delivering highly sensitive, reproducible, and precise diagnostic results.

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来源期刊
Expert Review of Proteomics
Expert Review of Proteomics 生物-生化研究方法
CiteScore
7.60
自引率
0.00%
发文量
20
审稿时长
6-12 weeks
期刊介绍: Expert Review of Proteomics (ISSN 1478-9450) seeks to collect together technologies, methods and discoveries from the field of proteomics to advance scientific understanding of the many varied roles protein expression plays in human health and disease. The journal coverage includes, but is not limited to, overviews of specific technological advances in the development of protein arrays, interaction maps, data archives and biological assays, performance of new technologies and prospects for future drug discovery. The journal adopts the unique Expert Review article format, offering a complete overview of current thinking in a key technology area, research or clinical practice, augmented by the following sections: Expert Opinion - a personal view on the most effective or promising strategies and a clear perspective of future prospects within a realistic timescale Article highlights - an executive summary cutting to the author''s most critical points.
期刊最新文献
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