评估外周血极小干细胞对治疗卵巢早衰的疗效:试点研究。

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Jornal Brasileiro de Reproducao Assistida Pub Date : 2024-08-26 DOI:10.5935/1518-0557.20240021
Nasrin Saharkhiz, Nazanin Hajizadeh, Jahan Saheb Alkhafaji, Mohammad Hossein Mohammadi
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引用次数: 0

摘要

目的:卵巢早衰(POF)是一种严重的健康问题,具有多种负面影响,会恶化患者的生活。本研究旨在评估从外周血中提取的间充质干细胞(MSCs)治疗卵巢早衰妇女的疗效:本研究是一项试验性研究,对象是40岁以下的卵巢早衰女性。研究参与者使用从外周血中提取的间充质干细胞进行了为期4个月的细胞治疗。血清卵泡刺激素(FSH)、雌二醇(E2)、抗缪勒氏激素(AMH)和前区卵泡计数(AFC)是主要的研究结果,分别在极小干细胞干预的基线、第2个月和第4个月进行评估:基线时,FSH的平均血清水平为45.0 (12.1) mIU/mL,在研究期间持续下降,在第四个月达到33.2 (12.4) mIU/mL。干预前,AMH 平均水平为 0.10 纳克/毫升,第二个月升至 0.13 纳克/毫升,第四个月升至 0.15 纳克/毫升。基线 E2 平均水平为 85.7 (23.6) pg/ml,第四个月平均 E2 水平降至 77.2 (25.6) pg/ml。基线时,AFC 的平均数量为 2.0(0.8)。在第二个月,我们观察到AFC逐渐增加(平均AFC=2.2),四个月后增加到3.1(1.8),这是月经恢复的最高峰,10%的研究参与者观察到怀孕:结论:间充质干细胞可明显改善POF妇女的激素分泌。结论:间充质干细胞可明显改善POF妇女的激素分泌。在POF妇女中植入间充质干细胞可增加AMH和AFC,同时降低E2和FSH的血清水平。间充质干细胞还能使 POF 妇女恢复月经并怀孕。
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Evaluation of the therapeutic effect of very small stem cells from peripheral blood on the treatment of Premature Ovarian Failure: A pilot study.

Objective: Premature ovary failure (POF) is a severe health condition with multiple negative outcomes, which deteriorate a patient's life. The current study aimed to evaluate the therapeutic effect of mesenchymal stem cells (MSCs) derived from peripheral blood in the treatment of women with the POF background.

Methods: The current study was a pilot study carried-out on women younger than 40 with premature ovarian failure. Study participants underwent 4-months cell therapy using Mesenchymal stem cells extracted from peripheral bloods. Serum level of Follicle-stimulating hormone (FSH), Estradiol (E2), Anti-mullerian hormone (AMH), and Antral follicle count (AFC) were the main investigated outcomes that were assessed at baseline, month two and month four of the very small stem cell intervention.

Results: Average serum level of FSH was 45.0 (12.1) mIU/mL at baseline and continually decreased during the study and reached 33.2 (12.4) mIU/mL in the fourth month. The average AMH level was 0.10 ng/mL prior to the intervention and increased to 0.13 ng/mL in the 2nd month and 0.15 ng/mL in the fourth month. The level E2 was 85.7 (23.6) pg/ml on average at baseline, while the average E2 reduced to 77.2 (25.6) pg/ml in the fourth month. Average number of AFC was 2.0 (0.8) at baseline. We observed a gradual increase in the second month (Mean AFC=2.2) and after four months it increased to 3.1 (1.8) as the highest menstrual restoration and pregnancy was observed in 10% of our study participants.

Conclusions: MSCs could significantly improve hormone secretion in women with POF. Implantation of MSCs in women with POF background was associated with an increase in AMH and AFC, while it downed the serum level of E2 and FSH. MSCs could also lead to menstrual restoration and pregnancy in women with POF.

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