半乳糖胺和甘露胺是细菌和真菌胞外聚合物物质的组成部分。

IF 5.1 Q1 ECOLOGY ISME communications Pub Date : 2024-03-22 eCollection Date: 2024-01-01 DOI:10.1093/ismeco/ycae038
Rebeca Leme Oliva, Carla Vogt, Tábata Aline Bublitz, Tessa Camenzind, Jens Dyckmans, Rainer Georg Joergensen
{"title":"半乳糖胺和甘露胺是细菌和真菌胞外聚合物物质的组成部分。","authors":"Rebeca Leme Oliva, Carla Vogt, Tábata Aline Bublitz, Tessa Camenzind, Jens Dyckmans, Rainer Georg Joergensen","doi":"10.1093/ismeco/ycae038","DOIUrl":null,"url":null,"abstract":"<p><p>Extracellular polymeric substances (EPS) are produced by microorganisms and interact to form a complex matrix called biofilm. In soils, EPS are important contributors to the microbial necromass and, thus, to soil organic carbon (SOC). Amino sugars (AS) are used as indicators for microbial necromass in soil, although the origin of galactosamine and mannosamine is largely unknown. However, indications exist that they are part of EPS. In this study, two bacteria and two fungi were grown in starch medium either with or without a quartz matrix to induce EPS production. Each culture was separated in two fractions: one that directly underwent AS extraction (containing AS from both biomass and EPS), and another that first had EPS extracted, followed then by AS determination (exclusively containing AS from EPS). We did not observe a general effect of the quartz matrix neither of microbial type on AS production. The quantified amounts of galactosamine and mannosamine in the EPS fraction represented on average 100% of the total amounts of these two AS quantified in cell cultures, revealing they are integral parts of the biofilm. In contrast, muramic acid and glucosamine were also quantified in the EPS, but with much lower contribution rates to total AS production, of 18% and 33%, respectively, indicating they are not necessarily part of EPS. Our results allow a meaningful ecological interpretation of mannosamine and galactosamine data in the future as indicators of microbial EPS, and also attract interest of future studies to investigate the role of EPS to SOC and its dynamics.</p>","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":null,"pages":null},"PeriodicalIF":5.1000,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11014887/pdf/","citationCount":"0","resultStr":"{\"title\":\"Galactosamine and mannosamine are integral parts of bacterial and fungal extracellular polymeric substances.\",\"authors\":\"Rebeca Leme Oliva, Carla Vogt, Tábata Aline Bublitz, Tessa Camenzind, Jens Dyckmans, Rainer Georg Joergensen\",\"doi\":\"10.1093/ismeco/ycae038\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Extracellular polymeric substances (EPS) are produced by microorganisms and interact to form a complex matrix called biofilm. In soils, EPS are important contributors to the microbial necromass and, thus, to soil organic carbon (SOC). Amino sugars (AS) are used as indicators for microbial necromass in soil, although the origin of galactosamine and mannosamine is largely unknown. However, indications exist that they are part of EPS. In this study, two bacteria and two fungi were grown in starch medium either with or without a quartz matrix to induce EPS production. Each culture was separated in two fractions: one that directly underwent AS extraction (containing AS from both biomass and EPS), and another that first had EPS extracted, followed then by AS determination (exclusively containing AS from EPS). We did not observe a general effect of the quartz matrix neither of microbial type on AS production. The quantified amounts of galactosamine and mannosamine in the EPS fraction represented on average 100% of the total amounts of these two AS quantified in cell cultures, revealing they are integral parts of the biofilm. In contrast, muramic acid and glucosamine were also quantified in the EPS, but with much lower contribution rates to total AS production, of 18% and 33%, respectively, indicating they are not necessarily part of EPS. Our results allow a meaningful ecological interpretation of mannosamine and galactosamine data in the future as indicators of microbial EPS, and also attract interest of future studies to investigate the role of EPS to SOC and its dynamics.</p>\",\"PeriodicalId\":73516,\"journal\":{\"name\":\"ISME communications\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.1000,\"publicationDate\":\"2024-03-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11014887/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ISME communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1093/ismeco/ycae038\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"ECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ISME communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/ismeco/ycae038","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

胞外聚合物(EPS)由微生物产生,并相互作用形成一种称为生物膜的复杂基质。在土壤中,EPS 是微生物坏死物质的重要来源,因此也是土壤有机碳 (SOC) 的重要来源。氨基糖(AS)被用作土壤中微生物尸体的指标,尽管半乳糖胺和甘露胺的来源在很大程度上还不清楚。不过,有迹象表明它们是 EPS 的一部分。在本研究中,两种细菌和两种真菌在淀粉培养基中生长,无论是否有石英基质诱导 EPS 的产生。每个培养物被分成两部分:一部分直接进行 AS 提取(从生物质和 EPS 中都含有 AS),另一部分先提取 EPS,然后进行 AS 测定(仅从 EPS 中含有 AS)。我们没有观察到石英基质或微生物类型对 AS 产量的普遍影响。EPS部分中半乳糖胺和甘露胺的定量平均占细胞培养物中这两种AS定量总量的100%,这表明它们是生物膜不可或缺的组成部分。与此相反,EPS 中也定量检测到了氨基甲酸和氨基葡萄糖,但它们对 AS 总产量的贡献率要低得多,分别为 18% 和 33%,这表明它们不一定是 EPS 的组成部分。我们的研究结果使甘露糖胺和半乳糖胺数据作为微生物 EPS 的指标在未来能得到有意义的生态学解释,同时也引起了未来研究对 EPS 在 SOC 中的作用及其动态的兴趣。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Galactosamine and mannosamine are integral parts of bacterial and fungal extracellular polymeric substances.

Extracellular polymeric substances (EPS) are produced by microorganisms and interact to form a complex matrix called biofilm. In soils, EPS are important contributors to the microbial necromass and, thus, to soil organic carbon (SOC). Amino sugars (AS) are used as indicators for microbial necromass in soil, although the origin of galactosamine and mannosamine is largely unknown. However, indications exist that they are part of EPS. In this study, two bacteria and two fungi were grown in starch medium either with or without a quartz matrix to induce EPS production. Each culture was separated in two fractions: one that directly underwent AS extraction (containing AS from both biomass and EPS), and another that first had EPS extracted, followed then by AS determination (exclusively containing AS from EPS). We did not observe a general effect of the quartz matrix neither of microbial type on AS production. The quantified amounts of galactosamine and mannosamine in the EPS fraction represented on average 100% of the total amounts of these two AS quantified in cell cultures, revealing they are integral parts of the biofilm. In contrast, muramic acid and glucosamine were also quantified in the EPS, but with much lower contribution rates to total AS production, of 18% and 33%, respectively, indicating they are not necessarily part of EPS. Our results allow a meaningful ecological interpretation of mannosamine and galactosamine data in the future as indicators of microbial EPS, and also attract interest of future studies to investigate the role of EPS to SOC and its dynamics.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Metagenomic insights into inhibition of soil microbial carbon metabolism by phosphorus limitation during vegetation succession. Introduction into natural environments shifts the gut microbiome of captivity-raised filter-feeding bivalves. Metagenomic and -transcriptomic analyses of microbial nitrogen transformation potential, and gene expression in Swiss lake sediments. Biological and experimental factors that define the effectiveness of microbial inoculation on plant traits: a meta-analysis. Rhizospheric miRNAs affect the plant microbiota.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1