一氧化氮和钙离子在硫化氢影响大鼠空肠收缩活性中的作用

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology Biophysics Pub Date : 2024-03-07 DOI:10.1134/S0006350923050287
D. M. Sorokina, I. F. Shaidullov, A. R. Gizzatullin, F. G. Sitdikov, G. F. Sitdikova
{"title":"一氧化氮和钙离子在硫化氢影响大鼠空肠收缩活性中的作用","authors":"D. M. Sorokina,&nbsp;I. F. Shaidullov,&nbsp;A. R. Gizzatullin,&nbsp;F. G. Sitdikov,&nbsp;G. F. Sitdikova","doi":"10.1134/S0006350923050287","DOIUrl":null,"url":null,"abstract":"<p>The roles of nitric oxide, intra- and extracellular calcium in the effects of hydrogen sulfide on spontaneous and carbachol-induced contractions of rat jejunal preparation under conditions of isometric contraction were studied. A donor of H<sub>2</sub>S (sodium hydrosulfide) led to a decrease in the preparation tone, amplitude and frequency of spontaneous contractions, as well as parameters of the contractions caused by carbachol, a nonspecific agonist of acetylcholine receptors. The effect of the H<sub>2</sub>S donor persisted under conditions of the inhibition of endogenous NO synthesis with L-NAME, while in the presence of the action of SNAP (an NO donor), the effects of NaHS on the amplitudes of spontaneous and carbachol-induced contractions were less pronounced. A decrease in preparation tone under the effect of NaHS was prevented by dantrolene, a blocker of ryanodine receptors. The calcium-free solution decreased the inhibitory effect of NaHS on the contractions induced by the application of carbachol. It is suggested that the inhibitory effect of H<sub>2</sub>S is associated with the dynamics of intracellular concentration of calcium ions, and the interaction of NO and H<sub>2</sub>S occurs at the level of common targets of the effect of the two gases.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":null,"pages":null},"PeriodicalIF":4.0330,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Roles of Nitric Oxide and Calcium Ions in the Effects of Hydrogen Sulfide on the Contractile Activity of the Rat Jejunum\",\"authors\":\"D. M. Sorokina,&nbsp;I. F. Shaidullov,&nbsp;A. R. Gizzatullin,&nbsp;F. G. Sitdikov,&nbsp;G. F. Sitdikova\",\"doi\":\"10.1134/S0006350923050287\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The roles of nitric oxide, intra- and extracellular calcium in the effects of hydrogen sulfide on spontaneous and carbachol-induced contractions of rat jejunal preparation under conditions of isometric contraction were studied. A donor of H<sub>2</sub>S (sodium hydrosulfide) led to a decrease in the preparation tone, amplitude and frequency of spontaneous contractions, as well as parameters of the contractions caused by carbachol, a nonspecific agonist of acetylcholine receptors. The effect of the H<sub>2</sub>S donor persisted under conditions of the inhibition of endogenous NO synthesis with L-NAME, while in the presence of the action of SNAP (an NO donor), the effects of NaHS on the amplitudes of spontaneous and carbachol-induced contractions were less pronounced. A decrease in preparation tone under the effect of NaHS was prevented by dantrolene, a blocker of ryanodine receptors. The calcium-free solution decreased the inhibitory effect of NaHS on the contractions induced by the application of carbachol. It is suggested that the inhibitory effect of H<sub>2</sub>S is associated with the dynamics of intracellular concentration of calcium ions, and the interaction of NO and H<sub>2</sub>S occurs at the level of common targets of the effect of the two gases.</p>\",\"PeriodicalId\":493,\"journal\":{\"name\":\"Biophysics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.0330,\"publicationDate\":\"2024-03-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biophysics\",\"FirstCategoryId\":\"4\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S0006350923050287\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophysics","FirstCategoryId":"4","ListUrlMain":"https://link.springer.com/article/10.1134/S0006350923050287","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0

摘要

摘要 研究了一氧化氮、细胞内和细胞外钙在硫化氢对等长收缩条件下大鼠空肠自发收缩和卡巴胆碱诱导收缩的影响中的作用。硫化氢供体(硫氢化钠)可降低空肠准备的张力、自发收缩的幅度和频率,以及卡巴胆碱(一种乙酰胆碱受体的非特异性激动剂)引起的收缩参数。在用 L-NAME 抑制内源性 NO 合成的条件下,H2S 供体的作用持续存在,而在 SNAP(一种 NO 供体)的作用下,NaHS 对自发收缩和卡巴胆碱诱导的收缩幅度的影响不明显。阻断雷诺丁受体的丹曲林(dantrolene)可防止在 NaHS 作用下准备张力的降低。无钙溶液降低了 NaHS 对卡巴胆碱诱导的收缩的抑制作用。这表明 H2S 的抑制作用与细胞内钙离子浓度的动态变化有关,而 NO 和 H2S 的相互作用发生在两种气体作用的共同靶标水平上。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
The Roles of Nitric Oxide and Calcium Ions in the Effects of Hydrogen Sulfide on the Contractile Activity of the Rat Jejunum

The roles of nitric oxide, intra- and extracellular calcium in the effects of hydrogen sulfide on spontaneous and carbachol-induced contractions of rat jejunal preparation under conditions of isometric contraction were studied. A donor of H2S (sodium hydrosulfide) led to a decrease in the preparation tone, amplitude and frequency of spontaneous contractions, as well as parameters of the contractions caused by carbachol, a nonspecific agonist of acetylcholine receptors. The effect of the H2S donor persisted under conditions of the inhibition of endogenous NO synthesis with L-NAME, while in the presence of the action of SNAP (an NO donor), the effects of NaHS on the amplitudes of spontaneous and carbachol-induced contractions were less pronounced. A decrease in preparation tone under the effect of NaHS was prevented by dantrolene, a blocker of ryanodine receptors. The calcium-free solution decreased the inhibitory effect of NaHS on the contractions induced by the application of carbachol. It is suggested that the inhibitory effect of H2S is associated with the dynamics of intracellular concentration of calcium ions, and the interaction of NO and H2S occurs at the level of common targets of the effect of the two gases.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Biophysics
Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍: Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
期刊最新文献
Reductive Nitrosylation of Hemoglobin and Myoglobin and its Antioxidant Effect Supercomputer Simulation of Intramolecular Oscillations of Glycine, Diphenylalanine, and Tryptophan in an Electric Field of the Terahertz and Infrared Ranges On the Role of Priming in the Development of Modern Rehabilitation Technologies The Inhibitory Effect of Oxibiol on the Process of Protein Modification by Water-Soluble Products of Photo-Oxidative Destruction of Bisretinoid A2E An X-Ray Diffraction Study of Lipid Films with ICHPHAN
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1