D. M. Sorokina, I. F. Shaidullov, A. R. Gizzatullin, F. G. Sitdikov, G. F. Sitdikova
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引用次数: 0
摘要
摘要 研究了一氧化氮、细胞内和细胞外钙在硫化氢对等长收缩条件下大鼠空肠自发收缩和卡巴胆碱诱导收缩的影响中的作用。硫化氢供体(硫氢化钠)可降低空肠准备的张力、自发收缩的幅度和频率,以及卡巴胆碱(一种乙酰胆碱受体的非特异性激动剂)引起的收缩参数。在用 L-NAME 抑制内源性 NO 合成的条件下,H2S 供体的作用持续存在,而在 SNAP(一种 NO 供体)的作用下,NaHS 对自发收缩和卡巴胆碱诱导的收缩幅度的影响不明显。阻断雷诺丁受体的丹曲林(dantrolene)可防止在 NaHS 作用下准备张力的降低。无钙溶液降低了 NaHS 对卡巴胆碱诱导的收缩的抑制作用。这表明 H2S 的抑制作用与细胞内钙离子浓度的动态变化有关,而 NO 和 H2S 的相互作用发生在两种气体作用的共同靶标水平上。
The Roles of Nitric Oxide and Calcium Ions in the Effects of Hydrogen Sulfide on the Contractile Activity of the Rat Jejunum
The roles of nitric oxide, intra- and extracellular calcium in the effects of hydrogen sulfide on spontaneous and carbachol-induced contractions of rat jejunal preparation under conditions of isometric contraction were studied. A donor of H2S (sodium hydrosulfide) led to a decrease in the preparation tone, amplitude and frequency of spontaneous contractions, as well as parameters of the contractions caused by carbachol, a nonspecific agonist of acetylcholine receptors. The effect of the H2S donor persisted under conditions of the inhibition of endogenous NO synthesis with L-NAME, while in the presence of the action of SNAP (an NO donor), the effects of NaHS on the amplitudes of spontaneous and carbachol-induced contractions were less pronounced. A decrease in preparation tone under the effect of NaHS was prevented by dantrolene, a blocker of ryanodine receptors. The calcium-free solution decreased the inhibitory effect of NaHS on the contractions induced by the application of carbachol. It is suggested that the inhibitory effect of H2S is associated with the dynamics of intracellular concentration of calcium ions, and the interaction of NO and H2S occurs at the level of common targets of the effect of the two gases.
BiophysicsBiochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍:
Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.