{"title":"溶菌酶与 PGLU-PEG 嵌段聚合物的相互作用特点","authors":"L. Yu. Filatova, N. G. Balabushevich","doi":"10.1134/S1061933X23601336","DOIUrl":null,"url":null,"abstract":"<p>The interaction of hen egg white lysozyme and block copolymers of poly(L–glutamic acid sodium salt) and polyethylene glycol (PGLU<sub>10</sub>–PEG and PGLU<sub>100</sub>–PEG) have been studied in an aqueous medium and at a water–air interface. Different physicochemical methods have been used: turbidimetry, tensiometry, fluorimetry, circular dichroism spectroscopy, electrophoretic light scattering, and transmission electron microscopy. The formation of mixed adsorption layers at the water–air interface has been observed at block copolymer : enzyme molar ratios of at most 2 : 1. In an aqueous medium, complexation of lysozyme with PGLU–PEG block copolymers leads to the formation of core–shell PGLU<sub>10</sub>–PEG : lysozyme complexes and PGLU<sub>100</sub>–PEG : lysozyme complexes with molar compositions of 1 : 1 or 2 : 1. The possibility of regulating the properties of the enzyme–block copolymer interaction products makes it possible to develop strategies for the production of antibacterial drugs.</p>","PeriodicalId":521,"journal":{"name":"Colloid Journal","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Features of Interaction of Lysozyme with PGLU–PEG Block Copolymers\",\"authors\":\"L. Yu. Filatova, N. G. Balabushevich\",\"doi\":\"10.1134/S1061933X23601336\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The interaction of hen egg white lysozyme and block copolymers of poly(L–glutamic acid sodium salt) and polyethylene glycol (PGLU<sub>10</sub>–PEG and PGLU<sub>100</sub>–PEG) have been studied in an aqueous medium and at a water–air interface. Different physicochemical methods have been used: turbidimetry, tensiometry, fluorimetry, circular dichroism spectroscopy, electrophoretic light scattering, and transmission electron microscopy. The formation of mixed adsorption layers at the water–air interface has been observed at block copolymer : enzyme molar ratios of at most 2 : 1. In an aqueous medium, complexation of lysozyme with PGLU–PEG block copolymers leads to the formation of core–shell PGLU<sub>10</sub>–PEG : lysozyme complexes and PGLU<sub>100</sub>–PEG : lysozyme complexes with molar compositions of 1 : 1 or 2 : 1. The possibility of regulating the properties of the enzyme–block copolymer interaction products makes it possible to develop strategies for the production of antibacterial drugs.</p>\",\"PeriodicalId\":521,\"journal\":{\"name\":\"Colloid Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-05-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Colloid Journal\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S1061933X23601336\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Colloid Journal","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1134/S1061933X23601336","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Features of Interaction of Lysozyme with PGLU–PEG Block Copolymers
The interaction of hen egg white lysozyme and block copolymers of poly(L–glutamic acid sodium salt) and polyethylene glycol (PGLU10–PEG and PGLU100–PEG) have been studied in an aqueous medium and at a water–air interface. Different physicochemical methods have been used: turbidimetry, tensiometry, fluorimetry, circular dichroism spectroscopy, electrophoretic light scattering, and transmission electron microscopy. The formation of mixed adsorption layers at the water–air interface has been observed at block copolymer : enzyme molar ratios of at most 2 : 1. In an aqueous medium, complexation of lysozyme with PGLU–PEG block copolymers leads to the formation of core–shell PGLU10–PEG : lysozyme complexes and PGLU100–PEG : lysozyme complexes with molar compositions of 1 : 1 or 2 : 1. The possibility of regulating the properties of the enzyme–block copolymer interaction products makes it possible to develop strategies for the production of antibacterial drugs.
期刊介绍：
Colloid Journal (Kolloidnyi Zhurnal) is the only journal in Russia that publishes the results of research in the area of chemical science dealing with the disperse state of matter and surface phenomena in disperse systems. The journal covers experimental and theoretical works on a great variety of colloid and surface phenomena: the structure and properties of interfaces; adsorption phenomena and structure of adsorption layers of surfactants; capillary phenomena; wetting films; wetting and spreading; and detergency. The formation of colloid systems, their molecular-kinetic and optical properties, surface forces, interaction of colloidal particles, stabilization, and criteria of stability loss of different disperse systems (lyosols and aerosols, suspensions, emulsions, foams, and micellar systems) are also topics of the journal. Colloid Journal also includes the phenomena of electro- and diffusiophoresis, electro- and thermoosmosis, and capillary and reverse osmosis, i.e., phenomena dealing with the existence of diffusion layers of molecules and ions in the vicinity of the interface.
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