槲皮素可改善鸡胚胎原代肝细胞中的脂质沉积。

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE British Poultry Science Pub Date : 2024-08-01 Epub Date: 2024-05-10 DOI:10.1080/00071668.2024.2332717
Y Feng, C Zhao, T Li, M Wang, B R Serrano, A R Barcenas, L Qu, W Zhao, M Shen
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引用次数: 0

摘要

1.过多脂肪的积累在非酒精性脂肪肝(NAFLD)的发病过程中起着一定的作用,而植物性饲料添加剂有可能改善这种情况。本研究涉及从鸡胚胎中分离和培养原代肝细胞,以建立油酸/地塞米松(OA/DEX)诱导的肝脂肪变性模型。在使用不同浓度的槲皮素(Que)处理后,使用尼罗红染色法、油红 O 染色法和细胞计数试剂盒 -8 (CCK8) 评估脂质积累和细胞活力。结果表明,OA 能有效处理肝细胞中的脂质积累。当阙浓度为 1000 ng/ml 和 10 000 ng/ml 时,正常组和 OA/DEX 组的细胞增殖没有明显差异。甘油三酯和胆固醇(低密度和高密度)随着阙处理的进行而下降,在 10 000 ng/ml 浓度时下降幅度最大。 基因表达水平下降到与对照组相似的水平。Western 印迹数据显示,固醇调节元件结合蛋白 1(SREBP-1)蛋白的表达与其 mRNA 的表达水平相关。阙通过α丝氨酸/苏氨酸蛋白激酶(AKT)和细胞外信号调节激酶(ERK)途径缓解脂质积累。脂质相关基因(APOB、PPARα、CYP3A5 和 SREBP-1)的表达水平降至与对照组相似的水平。Western 印迹数据表明,SREBP-1 蛋白表达与其 mRNA 表达水平相关。 补充阙通过 AKT 和 ERK 信号通路改善 OA/DEX 诱导的高脂肝细胞的脂质积累。
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Quercetin ameliorates lipid deposition in primary hepatocytes of the chicken embryo.

1. The accumulation of excessive fat plays a role in the development of non-alcoholic fatty liver disease (NAFLD) and phytogenic feed additives have the potential to ameliorate this. This study involved the isolation and culture of primary hepatocytes from chicken embryos to establish a model of hepatic steatosis induced by oleic acid/dexamethasone (OA/DEX). Lipid accumulation and cell viability were assessed using Nile Red staining, Oil Red O staining and cell count Kit -8 (CCK8) following treatment with varying concentrations of quercetin (Que). The potential mechanism by which Que exerts its effects was preliminarily investigated.2. The results indicated that OA effectively treated lipid accumulation in hepatocytes. There was no notable variance in cell proliferation between the normal and OA/DEX groups when subjected to Que treatment at concentrations of 1000 ng/ml and 10 000 ng/ml. Triglycerides and cholesterol (low and high density) decreased with Que treatment, with the most substantial reduction observed at 10 000 ng/ml.3. Gene expression levels decreased to levels similar to those in the control groups. Western blot data demonstrated that sterol regulatory element-binding protein 1 (SREBP-1) protein expression correlated with its mRNA expression level. Que mitigated lipid accumulation through the alpha serine/threonine protein kinase (AKT) and extracellular signal-regulated kinase (ERK) pathways. Expression levels of lipid-related genes (APOB, PPARα, CYP3A5 and SREBP-1) decreased to levels similar to the control groups. Western blot data demonstrated that the SREBP-1 protein expression correlated with its mRNA expression level.4. Supplementation with Que ameliorated lipid accumulation through AKT and ERK signalling pathway in OA/DEX-induced high-fat hepatocytes.

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来源期刊
British Poultry Science
British Poultry Science 农林科学-奶制品与动物科学
CiteScore
3.90
自引率
5.00%
发文量
88
审稿时长
4.5 months
期刊介绍: From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .
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