罕见变异关联分析揭示了碳水化合物代谢紊乱在严重青少年特发性脊柱侧凸中的重要作用。

IF 3.5 2区 医学 Q2 GENETICS & HEREDITY Journal of Medical Genetics Pub Date : 2024-06-20 DOI:10.1136/jmg-2023-109667
Wen Wen, Zhengye Zhao, Zhifa Zheng, Sen Zhao, Hengqiang Zhao, Xi Cheng, Huakang Du, Ziquan Li, Shengru Wang, Guixing Qiu, Zhihong Wu, Terry Jianguo Zhang, Nan Wu
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引用次数: 0

摘要

背景:青少年特发性脊柱侧弯症(AIS)是受遗传影响最严重的脊柱侧弯症,会导致脊柱畸形而无脊椎畸形。然而,AIS 的分子病因仍不清楚:利用基因组/外显子组测序技术,我们研究了来自中国汉族队列的 368 名重度 AIS 患者(Cobb 角大于 40°)和 3794 名对照者。我们进行了基于基因和通路的加权罕见变异关联测试,以评估基因突变负担和已建立的生物通路。我们还对14名AIS患者和15名对照者的肌肉组织进行了差异表达分析,以进行验证:结果:与视网膜葡萄糖代谢有关的乳酸转运体SLC16A8被鉴定为新的严重AIS相关基因(p=3.08E-06,假发现率=0.009)。大多数有SLC16A8有害变异的AIS病例在脊柱侧弯之前都有早发性高度近视。基于通路的负荷测试还显示,多个碳水化合物代谢通路,尤其是半乳糖代谢通路,出现了显著的富集。这些基因中存在有害变异的患者脊柱曲线明显增大。与分解代谢过程和营养反应相关的基因在AIS病例和对照组之间的表达存在差异,这进一步证实了我们的基因组学发现:这项研究揭示了碳水化合物代谢中的基因变异在 AIS 发病过程中的关键作用,为其病因学和潜在治疗方法提供了新的视角。
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Rare variant association analyses reveal the significant contribution of carbohydrate metabolic disturbance in severe adolescent idiopathic scoliosis.

Background: Adolescent idiopathic scoliosis (AIS), the predominant genetic-influenced scoliosis, results in spinal deformities without vertebral malformations. However, the molecular aetiology of AIS remains unclear.

Methods: Using genome/exome sequencing, we studied 368 patients with severe AIS (Cobb angle >40°) and 3794 controls from a Han Chinese cohort. We performed gene-based and pathway-based weighted rare variant association tests to assess the mutational burden of genes and established biological pathways. Differential expression analysis of muscle tissues from 14 patients with AIS and 15 controls was served for validation.

Results: SLC16A8, a lactate transporter linked to retinal glucose metabolism, was identified as a novel severe AIS-associated gene (p=3.08E-06, false discovery rate=0.009). Most AIS cases with deleterious SLC16A8 variants demonstrated early onset high myopia preceding scoliosis. Pathway-based burden test also revealed a significant enrichment in multiple carbohydrate metabolism pathways, especially galactose metabolism. Patients with deleterious variants in these genes demonstrated a significantly larger spinal curve. Genes related to catabolic processes and nutrient response showed divergent expression between AIS cases and controls, reinforcing our genomic findings.

Conclusion: This study uncovers the pivotal role of genetic variants in carbohydrate metabolism in the development of AIS, unveiling new insights into its aetiology and potential treatment.

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来源期刊
Journal of Medical Genetics
Journal of Medical Genetics 医学-遗传学
CiteScore
7.60
自引率
2.50%
发文量
92
审稿时长
4-8 weeks
期刊介绍: Journal of Medical Genetics is a leading international peer-reviewed journal covering original research in human genetics, including reviews of and opinion on the latest developments. Articles cover the molecular basis of human disease including germline cancer genetics, clinical manifestations of genetic disorders, applications of molecular genetics to medical practice and the systematic evaluation of such applications worldwide.
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