厌氧条件下生长的慢性鼻炎患者铜绿假单胞菌分离物的抗生素耐药性增强

IF 1.6 4区医学 Q2 OTORHINOLARYNGOLOGY Laryngoscope Investigative Otolaryngology Pub Date : 2024-05-09 DOI:10.1002/lio2.1244

Jannatul Ferdoush Tuli PhD, Mahnaz Ramezanpour PhD, Clare Cooksley PhD, George Spyro Bouras BSc, Kazuhiro Ogi MD, PhD, Sholeh Feizi PhD, Roshan Nepal PhD, Alkis James Psaltis MD, PhD, Peter-John Wormald MD, Sarah Vreugde MD, PhD

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引用次数: 0

摘要

导言：在慢性鼻窦炎（CRS）中，鼻腔的充血和堵塞会导致鼻窦腔内的厌氧条件，这可能会促进入侵病原体的毒力基因和抗生素耐药基因的表达。铜绿假单胞菌是一种兼性厌氧菌，可引起严重的顽固性 CRS。在本研究中，我们旨在评估在需氧和厌氧条件下生长的铜绿假单胞菌分离株对CRS患者浮游生物和生物膜的耐药性。方法在需氧和厌氧条件下，以浮游生物和生物膜形式培养 CRS 患者的铜绿假单胞菌临床分离株（n = 25）。测定了浮游生物的最低抑菌浓度（MIC）和最低生物膜根除浓度（MBEC）。此外，还通过荧光素二乙酸酯测定法评估了两种条件下的代谢活性、水晶紫测定法评估了生物膜生物量和 eDNA 浓度。结果在厌氧条件下生长的铜绿微囊桿菌浮游细胞对庆大霉素的耐药性增强（p <.01），而在厌氧条件下生长的铜绿微囊桿菌生物膜对庆大霉素（p <.0001）和左氧氟沙星（p <.001）的 MBEC 值显著增加。厌氧生物膜的代谢活性明显高于需氧生物膜（p < .0001）。然而，在有氧条件下生长的分离物的生物膜生物量高于厌氧条件（p < .5）。结论在厌氧条件下生长的 CRS 患者铜绿假单胞菌分离株对抗生素的耐药性明显增强，代谢活性提高，但生物膜生物量降低。证据级别 NA。

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Increased antibiotic resistance of Pseudomonas aeruginosa isolates from chronic rhinosinusitis patients grown in anaerobic conditions

Introduction

In chronic rhinosinusitis (CRS), the congestion and blockage of the nose can cause anaerobic conditions within the sinus cavities which may promote the expression of virulence and antibiotic resistance genes in invading pathogens. Pseudomonas aeruginosa is a facultative anaerobic bacteria and causes severe recalcitrant CRS. In this study, we aimed to evaluate the antimicrobial resistance of P. aeruginosa isolates of CRS patients in planktonic and biofilm form grown in aerobic and anaerobic conditions.

Methods

P. aeruginosa clinical isolates of CRS patients (n = 25) were grown in planktonic and biofilm form in aerobic and anaerobic conditions. Minimum inhibitory concentrations (MIC) of planktonic forms and minimum biofilm eradication concentrations (MBEC) were determined. Additionally, metabolic activity by fluorescein diacetate assay, biofilm biomass by crystal violet assay and eDNA concentration were assessed in both conditions.

Results

P. aeruginosa planktonic cells grown in anaerobic condition exhibited increased gentamicin resistance (p < .01), whereas P. aeruginosa biofilms grown in anaerobic condition displayed significantly increased MBEC values for gentamicin (p < .0001) and levofloxacin (p < .001). The metabolic activity of anaerobic biofilms was significantly higher compared with aerobic biofilms (p < .0001). However, the biofilm biomass of isolates grown in aerobic conditions was higher than anaerobic conditions (p < .5).

Conclusion

P. aeruginosa isolates from CRS patients grown in anaerobic conditions showed significantly increased resistance to antibiotics with an increased metabolic activity but decreased biofilm biomass.