上皮细胞与白色念珠菌相互作用的同位素标记三维拉曼共聚焦成像和原子力显微镜研究。

IF 4.2 2区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2024-05-09 DOI:10.1016/j.nano.2024.102750
Sarmiza Elena Stanca , Selene Mogavero , Wolfgang Fritzsche , Christoph Krafft , Bernhard Hube , Jürgen Popp
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引用次数: 0

摘要

人类致病真菌白色念珠菌在表皮感染过程中会损害上皮细胞。在这里,我们利用三维序列共焦拉曼光谱成像和原子力显微镜研究了白念珠菌野生型细胞、分泌的白念珠菌多肽毒素念珠菌溶血素以及缺乏念珠菌溶血素的突变细胞与上皮细胞的相互作用。造成上皮细胞损伤的念珠菌素在其氚化形式中显示出可识别的拉曼信号,其频率区域与细胞频率区域不同。在 2100-2200 cm-1 处的振动模式归因于碳-氘弯曲,而在 477 cm-1 处的振动模式归因于氮-氘平面外弯曲,这两个振动模式在原子核周围都能找到。原子力显微镜可观察到细胞上 100 nm 深的病变,力-距离曲线表明,经念珠菌素培养后,孔周围的粘附力更强。念珠菌素的靶标是质膜,但在白念珠菌感染过程中也会出现在上皮细胞的细胞膜内。
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Isotope labeled 3D-Raman confocal imaging and atomic force microscopy study on epithelial cells interacting with the fungus Candida albicans

The human pathogenic fungus Candida albicans damages epithelial cells during superficial infections. Here we use three-dimensional-sequential-confocal Raman spectroscopic imaging and atomic force microscopy to investigate the interaction of C. albicans wild type cells, the secreted C. albicans peptide toxin candidalysin and mutant cells lacking candidalysin with epithelial cells. The candidalysin is responsible for epithelial cell damage and exhibits in its deuterated form an identifiable Raman signal in a frequency region distinct from the cellular frequency region. Vibration modes at 2100–2200 cm−1 attributed to carbon‑deuterium bending and at 477 cm−1, attributed to the nitrogen‑deuterium out-of-plane bending, found around the nucleus, can be assigned to deuterated candidalysin. Atomic force microscopy visualized 100 nm deep lesions on the cell and force-distance curves indicate the higher adhesion on pore surrounding after incubation with candidalysin. Candidalysin targets the plasma membrane, but is also found inside of the cytosol of epithelial cells during C. albicans infection.

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来源期刊
CiteScore
11.10
自引率
0.00%
发文量
133
审稿时长
42 days
期刊介绍: The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.
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