揭示钙黏蛋白染色的精确性：揭示硅氟的作用

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Plant-microbe Interactions Pub Date : 2024-08-01 Epub Date: 2024-08-09 DOI:10.1094/MPMI-04-24-0038-LE

Uwe Conrath

{"title":"揭示钙黏蛋白染色的精确性：揭示硅氟的作用","authors":"Uwe Conrath","doi":"10.1094/MPMI-04-24-0038-LE","DOIUrl":null,"url":null,"abstract":"Callose is a vital component in plant biology, contributing to essential processes like pollen maturation and defense against pathogens. However, misconceptions surrounding callose staining persist, particularly regarding the role of aniline blue. It is now known that commercial aniline blue contains sirofluor, and it is this fluorophore, rather than aniline blue itself, that is responsible for the observed fluorescence during callose detection. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.","PeriodicalId":19009,"journal":{"name":"Molecular Plant-microbe Interactions","volume":" ","pages":"595"},"PeriodicalIF":3.4000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unlocking Precision in Callose Staining: Unveiling the Role of Sirofluor.\",\"authors\":\"Uwe Conrath\",\"doi\":\"10.1094/MPMI-04-24-0038-LE\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Callose is a vital component in plant biology, contributing to essential processes like pollen maturation and defense against pathogens. However, misconceptions surrounding callose staining persist, particularly regarding the role of aniline blue. It is now known that commercial aniline blue contains sirofluor, and it is this fluorophore, rather than aniline blue itself, that is responsible for the observed fluorescence during callose detection. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.\",\"PeriodicalId\":19009,\"journal\":{\"name\":\"Molecular Plant-microbe Interactions\",\"volume\":\" \",\"pages\":\"595\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Plant-microbe Interactions\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1094/MPMI-04-24-0038-LE\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/8/9 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Plant-microbe Interactions","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1094/MPMI-04-24-0038-LE","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/9 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

胼胝质是植物生物学的重要组成部分，有助于花粉成熟和抵御病原体等重要过程。然而，围绕胼胝质染色的误解一直存在，尤其是关于苯胺蓝的作用。现在已经知道，商用苯胺蓝中含有硅氟，在检测胼胝质时，正是这种荧光团而不是苯胺蓝本身产生了荧光。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Unlocking Precision in Callose Staining: Unveiling the Role of Sirofluor.

Callose is a vital component in plant biology, contributing to essential processes like pollen maturation and defense against pathogens. However, misconceptions surrounding callose staining persist, particularly regarding the role of aniline blue. It is now known that commercial aniline blue contains sirofluor, and it is this fluorophore, rather than aniline blue itself, that is responsible for the observed fluorescence during callose detection. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Molecular Plant-microbe Interactions 生物-生化与分子生物学

CiteScore

7.00

自引率

2.90%

发文量

250

审稿时长

3 months

期刊介绍： Molecular Plant-Microbe Interactions® (MPMI) publishes fundamental and advanced applied research on the genetics, genomics, molecular biology, biochemistry, and biophysics of pathological, symbiotic, and associative interactions of microbes, insects, nematodes, or parasitic plants with plants.