人类附睾蛋白 4--用于肺癌诊断和预后监测的新型潜在生物标记物

IF 1.9 4区医学 Q3 RESPIRATORY SYSTEM Clinical Respiratory Journal Pub Date : 2024-05-14 DOI:10.1111/crj.13774

Tingting Zhang, Lanhe Chu, Wenchong Tan, Cuiping Ye, Hangming Dong

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引用次数: 0

摘要

目的：探讨人附睾蛋白4（HE4）在诊断和监测肺癌预后中的应用价值：本研究旨在探讨人附睾蛋白4（HE4）在肺癌诊断和预后监测中的应用价值：首先，利用TCGA（The Cancer Genome Atlas）数据库分析肺癌组织中乳清酸性蛋白4-二硫键核心域2（WFDC2）的基因表达水平。随后，研究人员共招募了 160 人，分为三组：肺癌组（80 人）、良性病变组（40 人）和健康对照组（40 人）。采用电化学发光免疫测定法对血清 HE4 水平和其他生物标志物进行量化。此外，还通过免疫组织化学（IHC）分析了组织中 HE4 的表达。体外培养人气道上皮细胞（人支气管上皮细胞 [HBE]）和各种肺癌细胞系（SPC/PC9/A594/H520），通过免疫印迹（WB）检测 HE4 的水平：结果：对TCGA和UALCAN（阿拉巴马大学伯明翰分校癌症数据分析门户网站）数据库的分析表明，WFDC2基因表达水平在肺癌组织中上调（p 结论：血清HE4是肺癌的一个重要靶标：血清 HE4 是一种很有前景的新型生物标记物，可用于肺癌的诊断和预后评估。

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Human epididymis protein 4, a novel potential biomarker for diagnostic and prognosis monitoring of lung cancer

Objective

This study aimed to explore the application value of human epididymis protein 4 (HE4) in diagnosing and monitoring the prognosis of lung cancer.

Methods

First, TCGA (The Cancer Genome Atlas) databases were used to analyze whey-acidic-protein 4-disulfide bond core domain 2 (WFDC2) gene expression levels in lung cancer tissues. Then, a total of 160 individuals were enrolled, categorized into three groups: the lung cancer group (n = 80), the benign lesions group (n = 40), and the healthy controls group (n = 40). Serum HE4 levels and other biomarkers were quantified using an electro-chemiluminescent immunoassay. Additionally, the expression of HE4 in tissues was analyzed through immunohistochemistry (IHC). In vitro cultures of human airway epithelial (human bronchial epithelial [HBE]) cells and various lung cancer cell lines (SPC/PC9/A594/H520) were utilized to detect HE4 levels via western blot (WB).

Results

Analysis of the TCGA and UALCAN (The University of Alabama at Birmingham Cancer Data Analysis Portal) databases showed that WFDC2 gene expression levels were upregulated in lung cancer tissues (p < 0.01). Compared with the control group and the benign group, HE4 was significantly higher in the serum of patients with lung cancer (p < 0.001). Receiver operating characteristic (ROC) analysis confirmed that HE4 had better diagnostic efficacy than classical markers in the differential diagnosis of lung cancer and benign lesions and had the highest diagnostic value in lung adenocarcinoma (area under the ROC curve [AUC] = 0.826). HE4 increased in early lung cancer and positively correlated with poor prognosis (p < 0.001). Moreover, the results of WB and IHC revealed that the expression of HE4 was increased in lung cancer cells (SPC/A549/H520) and lung cancer tissues but decreased in PC9 cells with a lack of exon EGFR19 (p < 0.05).

Conclusion

Serum HE4 emerges as a promising novel biomarker for the diagnosis and prognosis assessment of lung cancer.

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来源期刊

Clinical Respiratory Journal 医学-呼吸系统

CiteScore

3.70

自引率

0.00%

发文量

104

审稿时长

>12 weeks

期刊介绍： Overview Effective with the 2016 volume, this journal will be published in an online-only format. Aims and Scope The Clinical Respiratory Journal (CRJ) provides a forum for clinical research in all areas of respiratory medicine from clinical lung disease to basic research relevant to the clinic. We publish original research, review articles, case studies, editorials and book reviews in all areas of clinical lung disease including: Asthma Allergy COPD Non-invasive ventilation Sleep related breathing disorders Interstitial lung diseases Lung cancer Clinical genetics Rhinitis Airway and lung infection Epidemiology Pediatrics CRJ provides a fast-track service for selected Phase II and Phase III trial studies. Keywords Clinical Respiratory Journal, respiratory, pulmonary, medicine, clinical, lung disease, Abstracting and Indexing Information Academic Search (EBSCO Publishing) Academic Search Alumni Edition (EBSCO Publishing) Embase (Elsevier) Health & Medical Collection (ProQuest) Health Research Premium Collection (ProQuest) HEED: Health Economic Evaluations Database (Wiley-Blackwell) Hospital Premium Collection (ProQuest) Journal Citation Reports/Science Edition (Clarivate Analytics) MEDLINE/PubMed (NLM) ProQuest Central (ProQuest) Science Citation Index Expanded (Clarivate Analytics) SCOPUS (Elsevier)