Barbora Benoni, Jiří František Potužník, Anton Škríba, Roberto Benoni, Jana Trylcova, Matouš Tulpa, Kristína Spustová, Katarzyna Grab, Maria-Bianca Mititelu, Jan Pačes, Jan Weber, David Stanek, Joanna Kowalska, Lucie Bednarova, Zuzana Keckesova, Pavel Vopalensky, Lenka Gahurova and Hana Cahova*,
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引用次数: 0
摘要
烟酰胺腺嘌呤二核苷酸(NAD)是细胞新陈代谢的重要组成部分,同时也是各种 RNA 的另一种 5' 帽。然而,NAD RNA帽的功能仍在研究之中。我们研究了 HIV-1 感染细胞中 RNA 的 NAD 盖帽,因为 HIV-1 是造成 NAD/NADH 细胞池耗竭并导致细胞内糙皮病的罪魁祸首。通过在感染 HIV-1 和未感染 HIV-1 的细胞中应用 NAD captureSeq 协议,我们发现四种 snRNA(如 U1)和四种 snoRNA 在感染 HIV-1 后失去了 NAD 冠。在这里,我们提供了证据,证明 NAD 帽的存在会降低 U1/HIV-1 前 mRNA 双链的稳定性。此外,我们还证明了通过过量表达 NAD RNA 去帽酶 DXO 来减少 NAD 去帽 RNA 的数量会导致 HIV-1 感染性增加。这表明,NAD封顶对 HIV-1 不利,并在其感染性中发挥作用。
HIV-1 Infection Reduces NAD Capping of Host Cell snRNA and snoRNA
Nicotinamide adenine dinucleotide (NAD) is a critical component of the cellular metabolism and also serves as an alternative 5′ cap on various RNAs. However, the function of the NAD RNA cap is still under investigation. We studied NAD capping of RNAs in HIV-1-infected cells because HIV-1 is responsible for the depletion of the NAD/NADH cellular pool and causing intracellular pellagra. By applying the NAD captureSeq protocol to HIV-1-infected and uninfected cells, we revealed that four snRNAs (e.g., U1) and four snoRNAs lost their NAD cap when infected with HIV-1. Here, we provide evidence that the presence of the NAD cap decreases the stability of the U1/HIV-1 pre-mRNA duplex. Additionally, we demonstrate that reducing the quantity of NAD-capped RNA by overexpressing the NAD RNA decapping enzyme DXO results in an increase in HIV-1 infectivity. This suggests that NAD capping is unfavorable for HIV-1 and plays a role in its infectivity.
期刊介绍:
ACS Chemical Biology provides an international forum for the rapid communication of research that broadly embraces the interface between chemistry and biology.
The journal also serves as a forum to facilitate the communication between biologists and chemists that will translate into new research opportunities and discoveries. Results will be published in which molecular reasoning has been used to probe questions through in vitro investigations, cell biological methods, or organismic studies.
We welcome mechanistic studies on proteins, nucleic acids, sugars, lipids, and nonbiological polymers. The journal serves a large scientific community, exploring cellular function from both chemical and biological perspectives. It is understood that submitted work is based upon original results and has not been published previously.