老化人类股骨近端的骨胶原拉伸特性

IF 2.1 Q3 ENDOCRINOLOGY & METABOLISM Bone Reports Pub Date : 2024-05-13 DOI:10.1016/j.bonr.2024.101773
Stefan Bracher , Benjamin Voumard , Mathieu Simon , Tatiana Kochetkova , Michael Pretterklieber , Philippe Zysset
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引用次数: 0

摘要

尽管骨量在骨质疏松性骨折中起着主导作用,但必须彻底了解老化骨组织的特性,以改善骨质疏松症的管理。在这种情况下,胶原蛋白的含量和完整性被认为是重要的因素,尽管对脱矿密实骨的拉伸行为与其在原生矿化状态下的孔隙率和弹性特性相关的研究还很有限。因此,本研究旨在(i)研究矿化骨和胶原微观力学性能的年龄依赖性;(ii)测试胶原性能是否有助于矿化骨的力学性能,以及如果是,在多大程度上有助于矿化骨的力学性能。在水合条件下对每个切片的一个样本进行了单轴拉伸测试。首先,对原生样本进行弹性测试(0.25%应变),然后对脱矿后的样本进行测试,直至失效。使用微型计算机断层扫描、拉曼光谱和重量测量方法对第二个试样的形态和成分进行了评估。宏观拉伸特性显示,只有原生样本的弹性模量与年龄有关,而表观弹性模量与性别有关(p <0.01)。成分和形态分析发现,相对矿物重量(r = -0.24,p <0.05)和胶原无序比(I∼1670/I∼1640,r = 0.25,p <0.05)与年龄和性别有微弱但显著的相关性,骨体积分数与性别有较强的相关性,而矿物质含量评估结果基本一致。结果表明,包括胶原蛋白和非胶原蛋白在内的有机相的机械性能与供体年龄无关。观察到的相对矿物质重量的减少和胶原蛋白网络相应的整体刚性响应可能是由于矿物质-胶原蛋白连接数量的减少以及纤维外和纤体内矿化的缺乏造成了波浪状和胶原纤维预拉伸的丧失。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Bone collagen tensile properties of the aging human proximal femur

Despite the dominant role of bone mass in osteoporotic fractures, aging bone tissue properties must be thoroughly understood to improve osteoporosis management. In this context, collagen content and integrity are considered important factors, although limited research has been conducted on the tensile behavior of demineralized compact bone in relation to its porosity and elastic properties in the native mineralized state. Therefore, this study aims (i) at examining the age-dependency of mineralized bone and collagen micromechanical properties; (ii) to test whether, and if so to which extent, collagen properties contribute to mineralized bone mechanical properties.

Two cylindrical cortical bone samples from fresh frozen human anatomic donor material were extracted from 80 proximal diaphyseal sections from a cohort of 24 female and 19 male donors (57 to 96 years at death). One sample per section was tested in uniaxial tension under hydrated conditions. First, the native sample was tested elastically (0.25 % strain), and after demineralization, up to failure. Morphology and composition of the second specimen was assessed using micro-computed tomography, Raman spectroscopy, and gravimetric methods. Simple and multiple linear regression were employed to relate morphological, compositional, and mechanical variables with age and sex.

Macro-tensile properties revealed that only elastic modulus of native samples was age dependent whereas apparent elastic modulus was sex dependent (p < 0.01). Compositional and morphological analysis detected a weak but significant age and sex dependency of relative mineral weight (r = −0.24, p < 0.05) and collagen disorder ratio (I∼1670/I∼1640, r = 0.25, p < 0.05) and a strong sex dependency of bone volume fraction while generally showing consistent results in mineral content assessment. Young's modulus of demineralized bone was significantly related to tissue mineral density and Young's modulus of native bone.

The results indicate that mechanical properties of the organic phase, that include collagen and non-collagenous proteins, are independent of donor age. The observed reduction in relative mineral weight and corresponding overall stiffer response of the collagen network may be caused by a reduced number of mineral-collagen connections and a lack of extrafibrillar and intrafibrillar mineralization that induces a loss of waviness and a collagen fiber pre-stretch.

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来源期刊
Bone Reports
Bone Reports Medicine-Orthopedics and Sports Medicine
CiteScore
4.30
自引率
4.00%
发文量
444
审稿时长
57 days
期刊介绍: Bone Reports is an interdisciplinary forum for the rapid publication of Original Research Articles and Case Reports across basic, translational and clinical aspects of bone and mineral metabolism. The journal publishes papers that are scientifically sound, with the peer review process focused principally on verifying sound methodologies, and correct data analysis and interpretation. We welcome studies either replicating or failing to replicate a previous study, and null findings. We fulfil a critical and current need to enhance research by publishing reproducibility studies and null findings.
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