André L. A. da Costa, Mariana A. Soares, Talita G. B. Lourenço, Kamila Guimarães-Pinto, Alessandra D. Filardy, Adriana Miranda de Oliveira, Beatriz Gouvêa de Luca, D’ Angelo Carlo Magliano, Olga M. O. Araujo, Larissa Moura, Ricardo Tadeu Lopes, Ana Luisa Palhares de Miranda, Jorge L. M. Tributino, Ana Paula Vieira Colombo
{"title":"牙周病原体放线杆菌 JP2 与小鼠结肠白细胞减少和肠道微生物群失衡有关。","authors":"André L. A. da Costa, Mariana A. Soares, Talita G. B. Lourenço, Kamila Guimarães-Pinto, Alessandra D. Filardy, Adriana Miranda de Oliveira, Beatriz Gouvêa de Luca, D’ Angelo Carlo Magliano, Olga M. O. Araujo, Larissa Moura, Ricardo Tadeu Lopes, Ana Luisa Palhares de Miranda, Jorge L. M. Tributino, Ana Paula Vieira Colombo","doi":"10.1111/jre.13288","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Aim</h3>\n \n <p>Evidence suggests that translocation of oral pathogens through the oral–gut axis may induce intestinal dysbiosis. This study aimed to evaluate the impact of a highly leukotoxic <i>Aggregatibacter actinomycetemcomitans</i> (<i>Aa</i>) strain on the gut microbiota, intestinal mucosal integrity and immune system in healthy mice.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Eight-week-old male C57BL6 mice were divided into control (<i>n</i> = 16) and JP2 groups (<i>n</i> = 19), which received intragastric gavage with PBS and with a suspension of <i>Aa</i> JP2 (HK921), respectively, twice a week for 4 weeks. Colonic <i>lamina propria</i>, fecal material, serum, gingival tissues, and mandibles were obtained for analyses of leukocyte populations, inflammatory mediators, mucosal integrity, alveolar bone loss, and gut microbiota. Differences between groups for these parameters were examined by non-parametric tests.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The gut microbial richness and the number of colonic macrophages, neutrophils, and monocytes were significantly lower in <i>Aa</i> JP2-infected mice than in controls (<i>p</i> < .05). In contrast, infected animals showed higher abundance of <i>Clostridiaceae, Lactobacillus taiwanensis, Helicobacter rodentium</i>, higher levels of IL-6 expression in colonic tissues, and higher splenic MPO activity than controls (<i>p</i> < .05). No differences in tight junction expression, serum endotoxin levels, and colonic inflammatory cytokines were observed between groups. Infected animals presented also slightly more alveolar bone loss and gingival IL-6 levels than controls (<i>p</i> < .05).</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Based on this model, intragastric administration of <i>Aa</i> JP2 is associated with changes in the gut ecosystem of healthy hosts, characterized by less live/recruited myeloid cells, enrichment of the gut microbiota with pathobionts and decrease in commensals. Negligible levels of colonic pro-inflammatory cytokines, and no signs of mucosal barrier disruption were related to these changes.</p>\n </section>\n </div>","PeriodicalId":16715,"journal":{"name":"Journal of periodontal research","volume":"59 5","pages":"961-973"},"PeriodicalIF":3.4000,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Periodontal pathogen Aggregatibacter actinomycetemcomitans JP2 correlates with colonic leukocytes decrease and gut microbiome imbalance in mice\",\"authors\":\"André L. A. da Costa, Mariana A. Soares, Talita G. B. Lourenço, Kamila Guimarães-Pinto, Alessandra D. Filardy, Adriana Miranda de Oliveira, Beatriz Gouvêa de Luca, D’ Angelo Carlo Magliano, Olga M. O. Araujo, Larissa Moura, Ricardo Tadeu Lopes, Ana Luisa Palhares de Miranda, Jorge L. M. Tributino, Ana Paula Vieira Colombo\",\"doi\":\"10.1111/jre.13288\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Aim</h3>\\n \\n <p>Evidence suggests that translocation of oral pathogens through the oral–gut axis may induce intestinal dysbiosis. This study aimed to evaluate the impact of a highly leukotoxic <i>Aggregatibacter actinomycetemcomitans</i> (<i>Aa</i>) strain on the gut microbiota, intestinal mucosal integrity and immune system in healthy mice.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Eight-week-old male C57BL6 mice were divided into control (<i>n</i> = 16) and JP2 groups (<i>n</i> = 19), which received intragastric gavage with PBS and with a suspension of <i>Aa</i> JP2 (HK921), respectively, twice a week for 4 weeks. Colonic <i>lamina propria</i>, fecal material, serum, gingival tissues, and mandibles were obtained for analyses of leukocyte populations, inflammatory mediators, mucosal integrity, alveolar bone loss, and gut microbiota. Differences between groups for these parameters were examined by non-parametric tests.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The gut microbial richness and the number of colonic macrophages, neutrophils, and monocytes were significantly lower in <i>Aa</i> JP2-infected mice than in controls (<i>p</i> < .05). In contrast, infected animals showed higher abundance of <i>Clostridiaceae, Lactobacillus taiwanensis, Helicobacter rodentium</i>, higher levels of IL-6 expression in colonic tissues, and higher splenic MPO activity than controls (<i>p</i> < .05). No differences in tight junction expression, serum endotoxin levels, and colonic inflammatory cytokines were observed between groups. Infected animals presented also slightly more alveolar bone loss and gingival IL-6 levels than controls (<i>p</i> < .05).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>Based on this model, intragastric administration of <i>Aa</i> JP2 is associated with changes in the gut ecosystem of healthy hosts, characterized by less live/recruited myeloid cells, enrichment of the gut microbiota with pathobionts and decrease in commensals. Negligible levels of colonic pro-inflammatory cytokines, and no signs of mucosal barrier disruption were related to these changes.</p>\\n </section>\\n </div>\",\"PeriodicalId\":16715,\"journal\":{\"name\":\"Journal of periodontal research\",\"volume\":\"59 5\",\"pages\":\"961-973\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-05-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of periodontal research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jre.13288\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of periodontal research","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jre.13288","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Periodontal pathogen Aggregatibacter actinomycetemcomitans JP2 correlates with colonic leukocytes decrease and gut microbiome imbalance in mice
Aim
Evidence suggests that translocation of oral pathogens through the oral–gut axis may induce intestinal dysbiosis. This study aimed to evaluate the impact of a highly leukotoxic Aggregatibacter actinomycetemcomitans (Aa) strain on the gut microbiota, intestinal mucosal integrity and immune system in healthy mice.
Methods
Eight-week-old male C57BL6 mice were divided into control (n = 16) and JP2 groups (n = 19), which received intragastric gavage with PBS and with a suspension of Aa JP2 (HK921), respectively, twice a week for 4 weeks. Colonic lamina propria, fecal material, serum, gingival tissues, and mandibles were obtained for analyses of leukocyte populations, inflammatory mediators, mucosal integrity, alveolar bone loss, and gut microbiota. Differences between groups for these parameters were examined by non-parametric tests.
Results
The gut microbial richness and the number of colonic macrophages, neutrophils, and monocytes were significantly lower in Aa JP2-infected mice than in controls (p < .05). In contrast, infected animals showed higher abundance of Clostridiaceae, Lactobacillus taiwanensis, Helicobacter rodentium, higher levels of IL-6 expression in colonic tissues, and higher splenic MPO activity than controls (p < .05). No differences in tight junction expression, serum endotoxin levels, and colonic inflammatory cytokines were observed between groups. Infected animals presented also slightly more alveolar bone loss and gingival IL-6 levels than controls (p < .05).
Conclusion
Based on this model, intragastric administration of Aa JP2 is associated with changes in the gut ecosystem of healthy hosts, characterized by less live/recruited myeloid cells, enrichment of the gut microbiota with pathobionts and decrease in commensals. Negligible levels of colonic pro-inflammatory cytokines, and no signs of mucosal barrier disruption were related to these changes.
期刊介绍：
The Journal of Periodontal Research is an international research periodical the purpose of which is to publish original clinical and basic investigations and review articles concerned with every aspect of periodontology and related sciences. Brief communications (1-3 journal pages) are also accepted and a special effort is made to ensure their rapid publication. Reports of scientific meetings in periodontology and related fields are also published.
One volume of six issues is published annually.
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