E. Kritikaki, G. Terzis, Meera Soundararajan, I. Vogiatzis, D. Simoes
{"title":"萎缩性慢性阻塞性肺疾病与非萎缩性慢性阻塞性肺疾病之间肌肉细胞外基质 (ECM) 蛋白在阔筋膜肌纤维中的表达情况","authors":"E. Kritikaki, G. Terzis, Meera Soundararajan, I. Vogiatzis, D. Simoes","doi":"10.1183/23120541.00857-2023","DOIUrl":null,"url":null,"abstract":"Extracellular matrix proteins (ECM) are the major constituent of muscle cell microenvironment, imparting instructive signalling, steering cell behaviour, and controlling muscle regeneration. ECM remodelling is among the most affected signalling pathways in COPD and aged muscle. As a fraction of COPD patients present muscle atrophy, we questioned whether ECM composition would be altered in patients with peripheral muscle wasting (atrophic COPD) compared to those without muscle wasting (non-atrophic COPD).A set of ECM molecules with known impact on myogenesis were quantified in vastus lateralis muscle biopsies from 29 COPD patients (FEV1: 55±12% predicted) using ELISA and Realtime-PCR. COPD were grouped to atrophic or non-atrophic based on fat-free mass index (< or≥17 kg.m−2).Atrophic COPD patients presented a lower average vastus lateralis muscle fibre cross sectional area (3872±258 μm2) compared to non-atrophic COPD (4509±198 μm2). Gene expression of ECM molecules was found significantly lower in atrophic COPD compared to non-atrophic COPD for collagen type I chain (COL1A1), fibronectin (FN1), tenascin C (TNC),and biglycan (BGN).In terms of protein levels, there were no significant differences between the two COPD cohorts for any of the ECM molecules tested.Although atrophic COPD presented decreased contractile muscle tissue, the differences in ECM mRNA expression between atrophic and non-atrophic COPD were not translated at the protein level, potentially indicating an accumulation of long-lived ECM proteins and dysregulated proteostasis, as this is typically observed during deconditioning and ageing.","PeriodicalId":11739,"journal":{"name":"ERJ Open Research","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Expression of intramuscular extracellular matrix (ECM) proteins in vastus lateralis muscle fibres between atrophic and non-atrophic COPD\",\"authors\":\"E. Kritikaki, G. Terzis, Meera Soundararajan, I. Vogiatzis, D. Simoes\",\"doi\":\"10.1183/23120541.00857-2023\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Extracellular matrix proteins (ECM) are the major constituent of muscle cell microenvironment, imparting instructive signalling, steering cell behaviour, and controlling muscle regeneration. ECM remodelling is among the most affected signalling pathways in COPD and aged muscle. As a fraction of COPD patients present muscle atrophy, we questioned whether ECM composition would be altered in patients with peripheral muscle wasting (atrophic COPD) compared to those without muscle wasting (non-atrophic COPD).A set of ECM molecules with known impact on myogenesis were quantified in vastus lateralis muscle biopsies from 29 COPD patients (FEV1: 55±12% predicted) using ELISA and Realtime-PCR. COPD were grouped to atrophic or non-atrophic based on fat-free mass index (< or≥17 kg.m−2).Atrophic COPD patients presented a lower average vastus lateralis muscle fibre cross sectional area (3872±258 μm2) compared to non-atrophic COPD (4509±198 μm2). Gene expression of ECM molecules was found significantly lower in atrophic COPD compared to non-atrophic COPD for collagen type I chain (COL1A1), fibronectin (FN1), tenascin C (TNC),and biglycan (BGN).In terms of protein levels, there were no significant differences between the two COPD cohorts for any of the ECM molecules tested.Although atrophic COPD presented decreased contractile muscle tissue, the differences in ECM mRNA expression between atrophic and non-atrophic COPD were not translated at the protein level, potentially indicating an accumulation of long-lived ECM proteins and dysregulated proteostasis, as this is typically observed during deconditioning and ageing.\",\"PeriodicalId\":11739,\"journal\":{\"name\":\"ERJ Open Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-05-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ERJ Open Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1183/23120541.00857-2023\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"RESPIRATORY SYSTEM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ERJ Open Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1183/23120541.00857-2023","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"RESPIRATORY SYSTEM","Score":null,"Total":0}
Expression of intramuscular extracellular matrix (ECM) proteins in vastus lateralis muscle fibres between atrophic and non-atrophic COPD
Extracellular matrix proteins (ECM) are the major constituent of muscle cell microenvironment, imparting instructive signalling, steering cell behaviour, and controlling muscle regeneration. ECM remodelling is among the most affected signalling pathways in COPD and aged muscle. As a fraction of COPD patients present muscle atrophy, we questioned whether ECM composition would be altered in patients with peripheral muscle wasting (atrophic COPD) compared to those without muscle wasting (non-atrophic COPD).A set of ECM molecules with known impact on myogenesis were quantified in vastus lateralis muscle biopsies from 29 COPD patients (FEV1: 55±12% predicted) using ELISA and Realtime-PCR. COPD were grouped to atrophic or non-atrophic based on fat-free mass index (< or≥17 kg.m−2).Atrophic COPD patients presented a lower average vastus lateralis muscle fibre cross sectional area (3872±258 μm2) compared to non-atrophic COPD (4509±198 μm2). Gene expression of ECM molecules was found significantly lower in atrophic COPD compared to non-atrophic COPD for collagen type I chain (COL1A1), fibronectin (FN1), tenascin C (TNC),and biglycan (BGN).In terms of protein levels, there were no significant differences between the two COPD cohorts for any of the ECM molecules tested.Although atrophic COPD presented decreased contractile muscle tissue, the differences in ECM mRNA expression between atrophic and non-atrophic COPD were not translated at the protein level, potentially indicating an accumulation of long-lived ECM proteins and dysregulated proteostasis, as this is typically observed during deconditioning and ageing.
期刊介绍:
ERJ Open Research is a fully open access original research journal, published online by the European Respiratory Society. The journal aims to publish high-quality work in all fields of respiratory science and medicine, covering basic science, clinical translational science and clinical medicine. The journal was created to help fulfil the ERS objective to disseminate scientific and educational material to its members and to the medical community, but also to provide researchers with an affordable open access specialty journal in which to publish their work.