小扁豆新品种 RKL 58F-3715:描述理想转基因分离株出现的案例研究

Sumer Singh Punia, Khajan Singh, Sarfraz Ahmad, Baldev Ram, Meenakshi Dheer, Preeti Verma, Vijay Singh Jat, Ramesh Kumar Solanki, Ashok Kumar Parihar, Shailesh Kumar Jain, Dalip, Rubina Khan, Shiv Kumar Agrawal, Sanjeev Gupta
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摘要

扁豆是世界上最重要的冷季谷物豆类之一。由于小扁豆的花为一夫一妻制,在自然条件下自然重组和分离的频率非常低。本研究描述了自发突变产生的理想转基因分离株。利用这些超变异突变体,从自然变异的后代中培育出了高产扁豆品种 RKL 58F-3715 (Kota Masoor 4;KM 4)。从 DPL 62 的种子生产地块中发现的单株偏差植株可能是自发突变。在世代交替过程中,后代出现了超变异分离株,尤其是种子形态特征,如大小、形状、颜色和质地。在改良血统和单种子后裔的严格选育下,育种周期产生了高产稳产的基因型 KM 4。从自然突变体中获得的超变异后代,在形态特征(如早熟、叶色浅、卷须突出)上与母本品种有明显区别。KM 4 的全年全国各地点加权平均谷物产量为 1865 公斤/公顷,比国家对照 DPL 62 优产 22.80%。该品种对主要病害(枯萎病和锈病)具有很强的抗性,因此是印度主要扁豆种植区商业化种植的耐久性基因型。
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New lentil variety RKL 58F-3715: a case study depicting the emergence of desirable transgressive segregants
Lentil is one of the world’s most important cool-season grain legumes. The frequency of natural recombinants and segregants under natural conditions is very low in lentil due to their cleistogamous flower. The present investigation depicted the emergence of desirable transgressive segregants obtained from spontaneous mutation. Exploiting these hypervariable mutants, a highyielding lentil variety RKL 58F-3715 (Kota Masoor 4; KM 4) was developed from the progeny of a natural variant. The identified single deviated plant from the seed production plot of DPL 62 was probably a spontaneous mutation. During generation advancement, the progenies showed hypervariable segregants, especially for seed morphological traits, such as size, shape, colour and texture. The breeding cycles following rigorous selection under modified pedigree and single seed descent delivered the high-yielding stable genotype KM 4. Hypervariable progenies were obtained from natural mutants, distinguished in morphological traits such as earliness, light foliage colour, and prominent tendrils from the parent variety. The national weighted mean over locations over the year of KM 4 for grain yield was 1865 kg/ha, with yield superiority of 22.80% over national check DPL 62. Considerable resistance against major diseases, i.e., wilt and rust, makes it a durable genotype for commercial cultivation in major lentil-growing areas of India.
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