Wesam T. Basal, Medhat El-Sakka, Neima K. El-Sonousy, Aliaa M. Issa
{"title":"石榴皮提取物对人肝癌 HepG2 细胞系的选择性凋亡和基因毒性作用","authors":"Wesam T. Basal, Medhat El-Sakka, Neima K. El-Sonousy, Aliaa M. Issa","doi":"10.1007/s13596-024-00753-2","DOIUrl":null,"url":null,"abstract":"<div><p>Finding a therapeutic agent that is selective, non-toxic to normal cells, and does not develop resistance or unpleasant side effects became the most challenging aspect in cancer therapy. Our study investigates the potential antitumorigenic effects of pomegranate peel extract against human hepatocellular carcinoma HepG<sub>2</sub> cell line. The effect of the extract on cell viability, DNA fragmentation, cell cycle arrest, and expression of cancer related genes in human hepatocellular carcinoma cell line (HepG<sub>2</sub>), and normal human hepatocyte cell line (THLE<sub>2</sub>) was evaluated using MTT, comet assay, flow cytometry and real time PCR, respectively. Unlike THLE<sub>2</sub> cells, the pomegranate peel extract significantly decreased cell viability accompanied by DNA fragmentation and cell cycle arrest in HepG<sub>2</sub> cells. It also significantly decreased the expression levels of Cyclooxygenase-2, B-cell lymphoma 2, vascular permeability factor, matrix metalloproteinase -9, cyclin B1, and cyclin dependent kinase1 while the expression levels of BCL2-associated X protein, and p21 genes were significantly increased. The obtained results suggest a potential antitumorigenic effect of pomegranate peel extract against HepG<sub>2</sub> cell lines.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 4","pages":"1053 - 1064"},"PeriodicalIF":1.8000,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Selective apoptotic and genotoxic effects of pomegranate peel extract against human hepatocellular carcinoma HepG2 cell line\",\"authors\":\"Wesam T. Basal, Medhat El-Sakka, Neima K. El-Sonousy, Aliaa M. Issa\",\"doi\":\"10.1007/s13596-024-00753-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Finding a therapeutic agent that is selective, non-toxic to normal cells, and does not develop resistance or unpleasant side effects became the most challenging aspect in cancer therapy. Our study investigates the potential antitumorigenic effects of pomegranate peel extract against human hepatocellular carcinoma HepG<sub>2</sub> cell line. The effect of the extract on cell viability, DNA fragmentation, cell cycle arrest, and expression of cancer related genes in human hepatocellular carcinoma cell line (HepG<sub>2</sub>), and normal human hepatocyte cell line (THLE<sub>2</sub>) was evaluated using MTT, comet assay, flow cytometry and real time PCR, respectively. Unlike THLE<sub>2</sub> cells, the pomegranate peel extract significantly decreased cell viability accompanied by DNA fragmentation and cell cycle arrest in HepG<sub>2</sub> cells. It also significantly decreased the expression levels of Cyclooxygenase-2, B-cell lymphoma 2, vascular permeability factor, matrix metalloproteinase -9, cyclin B1, and cyclin dependent kinase1 while the expression levels of BCL2-associated X protein, and p21 genes were significantly increased. The obtained results suggest a potential antitumorigenic effect of pomegranate peel extract against HepG<sub>2</sub> cell lines.</p></div>\",\"PeriodicalId\":7613,\"journal\":{\"name\":\"Advances in Traditional Medicine\",\"volume\":\"24 4\",\"pages\":\"1053 - 1064\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-05-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advances in Traditional Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13596-024-00753-2\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Traditional Medicine","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1007/s13596-024-00753-2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Selective apoptotic and genotoxic effects of pomegranate peel extract against human hepatocellular carcinoma HepG2 cell line
Finding a therapeutic agent that is selective, non-toxic to normal cells, and does not develop resistance or unpleasant side effects became the most challenging aspect in cancer therapy. Our study investigates the potential antitumorigenic effects of pomegranate peel extract against human hepatocellular carcinoma HepG2 cell line. The effect of the extract on cell viability, DNA fragmentation, cell cycle arrest, and expression of cancer related genes in human hepatocellular carcinoma cell line (HepG2), and normal human hepatocyte cell line (THLE2) was evaluated using MTT, comet assay, flow cytometry and real time PCR, respectively. Unlike THLE2 cells, the pomegranate peel extract significantly decreased cell viability accompanied by DNA fragmentation and cell cycle arrest in HepG2 cells. It also significantly decreased the expression levels of Cyclooxygenase-2, B-cell lymphoma 2, vascular permeability factor, matrix metalloproteinase -9, cyclin B1, and cyclin dependent kinase1 while the expression levels of BCL2-associated X protein, and p21 genes were significantly increased. The obtained results suggest a potential antitumorigenic effect of pomegranate peel extract against HepG2 cell lines.
期刊介绍：
Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
