一些大豆品种[Glycine max (L.) Merr.]及其杂交种的植物化学研究

Anila Juhi
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摘要

在本研究中,我们尝试将对大豆(Glycine max L.)变种间 F1 代杂交种及其亲本的植物化学研究集中在一起。这 11 个 F1 代杂交种是用 Bragg(BG)、NRC7(NR)、Punjab1(PN)、PUSA98-14(PU) 和 JS9560(JS) 五个大豆品种进行品种间杂交获得的。总酚含量在杂交品种 JSXNR 中最高,在 BGXNR 中最低。总单宁含量采用 FCR 法测定。总单宁含量在 NRXJS 中最高,在 BGXPN 中最低。此外,还用 Alcl3 试剂测定了总黄酮含量,用 DPPH 法测定了抗氧化活性。总黄酮含量在 PNXNR 中最高,在 PUXPN 中最低。抗氧化活性最高的是 JSXPN,为 50.31%,最低的是 NRXJS,为 39.08%。结果表明,除了蛋白质和油含量,酚含量也应被视为大豆种子的一个重要特征,并可作为大豆抗氧化活性的潜在选择标准。
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Phytochemical studies in some varieties of soybean [Glycine max (L.) Merr.] and their hybrids
In the present investigation, an attempt has been made to congregate the phytochemical studies done on intervarietal F1 hybrids of soybean (Glycine max L.) along with their parents. The eleven F1 hybrids were obtained by intervarietal crossing done using five varieties of soybean viz Bragg(BG), NRC7(NR), Punjab1(PN), PUSA98-14(PU) and JS9560(JS).Estimation of phenols was done by FCR Method using Gallic acid as standard. The total phenolic content was found highest in the cross JSXNR and lowest in BGXNR. Total tannin content was determined using FCR method. Total tannin content was highest in NRXJS and least in BGXPN. In addition, total flavonoids were determined using Alcl3 reagent and antioxidant activity by DPPH method. Total flavonoids was found maximum in PNXNR and least in PUXPN respectively. Highest Antioxidant Activity was 50.31% in JSXPN and lowest was 39.08% in NRXJS. The results suggested that besides protein and oil contents, the phenolic contents should also be considered as an important characteristic feature of soybean seeds, and as a potential selection criterion for antioxidant activity in soybean.
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