从钢制招牌中分离出的固氮菌在空气条件下产生生物氢

N. Aburai, Honami Tanaka, Hana Kohira, Tinami Sekine
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摘要

如果能够不依赖化石燃料而高效地生产氢气,那么氢气作为一种清洁燃料来源是非常有吸引力的。利用光合细菌进行生物制氢可实现环境友好型制氢,但目前受到耐低氧性等因素的限制。在本研究中,我们分离出一种新的细菌菌株,与在氮气或氩气气氛中的液相条件下相比,它能在气相条件下生产氢气。细菌菌株是从钢制招牌的刮屑中培养出来的。对菌株在气相和液相条件下的产氢情况进行了调查,随后进行了 DNA 测序,最终确定了 Cereibacter sp.气相条件是通过将细菌菌株装入滤膜并将滤膜放在浸过培养基的棉絮上实现的。气体环境对分离出的细菌菌株在气相和液相条件下的行为都有影响。Cereibacter sp. KGU-NF001 表现出良好的耐氧性,即使在含氧量为 12% 的环境中,也能保持 1.33 mL/mg/d 的产氢量。我们的研究结果表明,光合细菌可以在含氧气相条件下生产生物氢,这为降低我们对化石燃料的依赖提供了可能的途径。
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Biohydrogen Production under Aerial Conditions by a Nitrogen-Fixing Bacterium Isolated from a Steel Signboard
Hydrogen gas is attractive as a clean fuel source if it can be produced efficiently without relying on fossil fuels. Biohydrogen production using photosynthetic bacteria may enable environmentally friendly hydrogen production but is currently limited by factors such as low oxygen tolerance. In this study, we isolate a new strain of bacteria that can produce hydrogen under aerial-phase conditions compared with those under liquid-phase conditions in a nitrogen gas or an argon gas atmosphere. Bacterial strains were cultured from scrapings taken from a steel signboard. Investigation of the hydrogen production of the strains under aerial- and liquid-phase conditions and subsequent DNA sequencing led to identification of the bacterium Cereibacter sp. KGU-NF001. Aerial-phase conditions were achieved by filter membranes with the bacterial strains and placing the membranes on medium-soaked cotton wool. The gas atmosphere affected the behavior of the isolated bacterial strains under both aerial- and liquid-phase conditions. Cereibacter sp. KGU-NF001 showed promising oxygen tolerance and was able to maintain hydrogen production of 1.33 mL/mg/d even when the atmosphere contained 12% oxygen. Our findings illustrate that biohydrogen production may be achieved by photosynthetic bacteria under oxygen-containing aerial-phase conditions, indicating a possible pathway to help lower our reliance on fossil fuels.
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