用红外成像技术评估野生火箭草(Diplotaxis tenuifolia)在感染初期对镰刀菌枯萎病的反应

IF 2.3 Q1 AGRICULTURE, MULTIDISCIPLINARY ACS agricultural science & technology Pub Date : 2024-05-08 DOI:10.1021/acsagscitech.3c00582
Massimo Rippa*, Andrea Pasqualini, Gelsomina Manganiello, Sheridan Lois Woo, Pasquale Mormile and Catello Pane, 
{"title":"用红外成像技术评估野生火箭草(Diplotaxis tenuifolia)在感染初期对镰刀菌枯萎病的反应","authors":"Massimo Rippa*,&nbsp;Andrea Pasqualini,&nbsp;Gelsomina Manganiello,&nbsp;Sheridan Lois Woo,&nbsp;Pasquale Mormile and Catello Pane,&nbsp;","doi":"10.1021/acsagscitech.3c00582","DOIUrl":null,"url":null,"abstract":"<p >Wild rocket is a baby-leaf vegetable crop devoted to the high-convenience food chain for the preparation of ready-to-eat salads. Healthiness and ecological management of crops are the requirements increasingly demanded by the market. On the other hand, very intensive cultivations under greenhouse are prone to fungal soil-borne pathogen attacks, such as <i>Fusarium oxysporum</i> <i>f. sp.</i> <i>raphani</i>, the causal agent of wilting. Control strategies currently include chemical and nonchemical means, but the implementation of digital systems able to support detection of outbreaks and promote optimization of interventions may concur to increase efficacy of management strategies. Thermography, based on the recording and analysis of thermal energy emitted by plant canopy in the infrared spectral range, is a well-known imaging technique able to monitor plants at both proximal and remote scales providing information on incipient plant stresses. In this work, wild rocket plants subjected to artificial infection with pathogen conidia performed by a dipping or flooding method were monitored during the pathogenesis by both passive and active thermographic approaches. Canopy temperature changes were compared with symptom severity indices measured by visual inspection on both inoculated and control plants. As key result of this study, depending on the inoculum concentration, passive thermography allowed classifying infected plants 30–48 h post inoculation (hpi) by dipping, and in coherence with the disease symptoms detection by visual inspection. Similar results were found in the case of the flooding inoculation, where infected plants were detected by thermography at 48 h.p.i. Active thermography revealed a decrease in leaf heat capacity attributable to the fungal infection and the subsequent tissue colonization over time. These findings constitute a solid base of knowledge about the thermal imaging applied to wild rocket affected by Fusarium wilting, and they can contribute to the development of new remote sensing systems for the detection of primary outbreaks.</p>","PeriodicalId":93846,"journal":{"name":"ACS agricultural science & technology","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Infrared Imaging to Assess the Wild Rocket (Diplotaxis tenuifolia) Response to Fusarium Wilt in the Early Stages of Infection\",\"authors\":\"Massimo Rippa*,&nbsp;Andrea Pasqualini,&nbsp;Gelsomina Manganiello,&nbsp;Sheridan Lois Woo,&nbsp;Pasquale Mormile and Catello Pane,&nbsp;\",\"doi\":\"10.1021/acsagscitech.3c00582\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Wild rocket is a baby-leaf vegetable crop devoted to the high-convenience food chain for the preparation of ready-to-eat salads. Healthiness and ecological management of crops are the requirements increasingly demanded by the market. On the other hand, very intensive cultivations under greenhouse are prone to fungal soil-borne pathogen attacks, such as <i>Fusarium oxysporum</i> <i>f. sp.</i> <i>raphani</i>, the causal agent of wilting. Control strategies currently include chemical and nonchemical means, but the implementation of digital systems able to support detection of outbreaks and promote optimization of interventions may concur to increase efficacy of management strategies. Thermography, based on the recording and analysis of thermal energy emitted by plant canopy in the infrared spectral range, is a well-known imaging technique able to monitor plants at both proximal and remote scales providing information on incipient plant stresses. In this work, wild rocket plants subjected to artificial infection with pathogen conidia performed by a dipping or flooding method were monitored during the pathogenesis by both passive and active thermographic approaches. Canopy temperature changes were compared with symptom severity indices measured by visual inspection on both inoculated and control plants. As key result of this study, depending on the inoculum concentration, passive thermography allowed classifying infected plants 30–48 h post inoculation (hpi) by dipping, and in coherence with the disease symptoms detection by visual inspection. Similar results were found in the case of the flooding inoculation, where infected plants were detected by thermography at 48 h.p.i. Active thermography revealed a decrease in leaf heat capacity attributable to the fungal infection and the subsequent tissue colonization over time. These findings constitute a solid base of knowledge about the thermal imaging applied to wild rocket affected by Fusarium wilting, and they can contribute to the development of new remote sensing systems for the detection of primary outbreaks.</p>\",\"PeriodicalId\":93846,\"journal\":{\"name\":\"ACS agricultural science & technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-05-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS agricultural science & technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acsagscitech.3c00582\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS agricultural science & technology","FirstCategoryId":"1085","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsagscitech.3c00582","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

摘要

野生火箭菜是一种嫩叶蔬菜作物,专门用于制作即食沙拉的高便利食品链。市场对作物的健康和生态管理要求越来越高。另一方面,大棚内的密集栽培很容易受到土传真菌病原体的侵袭,例如枯萎病的病原体 Fusarium oxysporum f. sp. raphani。目前的控制策略包括化学和非化学手段,但实施能够支持检测疫情和促进优化干预措施的数字系统,可能会提高管理策略的效率。热成像技术基于对植物冠层在红外光谱范围内发出的热能的记录和分析,是一种著名的成像技术,能够在近距离和远距离监测植物,提供有关植物初期压力的信息。在这项工作中,通过被动和主动热成像方法,对采用浸渍或浸水方法人工感染病原体分生孢子的野生火箭草进行了病原发生过程的监测。将冠层温度变化与接种植株和对照植株通过目测测量的症状严重程度指数进行了比较。这项研究的主要结果是,根据接种物浓度的不同,被动热成像技术可在浸渍接种后 30-48 小时对受感染的植物进行分类,并与目测发现的病害症状保持一致。主动热成像显示,随着时间的推移,真菌感染和随后的组织定殖导致叶片热容量下降。这些发现为应用于受枯萎镰刀菌影响的野生火箭的热成像技术奠定了坚实的知识基础,并有助于开发新的遥感系统来检测原发性疫情。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Infrared Imaging to Assess the Wild Rocket (Diplotaxis tenuifolia) Response to Fusarium Wilt in the Early Stages of Infection

Wild rocket is a baby-leaf vegetable crop devoted to the high-convenience food chain for the preparation of ready-to-eat salads. Healthiness and ecological management of crops are the requirements increasingly demanded by the market. On the other hand, very intensive cultivations under greenhouse are prone to fungal soil-borne pathogen attacks, such as Fusarium oxysporum f. sp. raphani, the causal agent of wilting. Control strategies currently include chemical and nonchemical means, but the implementation of digital systems able to support detection of outbreaks and promote optimization of interventions may concur to increase efficacy of management strategies. Thermography, based on the recording and analysis of thermal energy emitted by plant canopy in the infrared spectral range, is a well-known imaging technique able to monitor plants at both proximal and remote scales providing information on incipient plant stresses. In this work, wild rocket plants subjected to artificial infection with pathogen conidia performed by a dipping or flooding method were monitored during the pathogenesis by both passive and active thermographic approaches. Canopy temperature changes were compared with symptom severity indices measured by visual inspection on both inoculated and control plants. As key result of this study, depending on the inoculum concentration, passive thermography allowed classifying infected plants 30–48 h post inoculation (hpi) by dipping, and in coherence with the disease symptoms detection by visual inspection. Similar results were found in the case of the flooding inoculation, where infected plants were detected by thermography at 48 h.p.i. Active thermography revealed a decrease in leaf heat capacity attributable to the fungal infection and the subsequent tissue colonization over time. These findings constitute a solid base of knowledge about the thermal imaging applied to wild rocket affected by Fusarium wilting, and they can contribute to the development of new remote sensing systems for the detection of primary outbreaks.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
2.80
自引率
0.00%
发文量
0
期刊最新文献
Issue Publication Information Issue Editorial Masthead Advancing Nanotechnology in Agriculture and Food: A Guide to Writing a Successful Manuscript Soil Potassium Sensor Using a Valinomycin-Decorated Reduced Graphene Oxide (rGO-v)-Based Field-Effect Transistor for Precision Farming Antifungal Activity of Vanillic Acid Grafted Chitosan Derivatives against Plant Pathogenic Fungi, Fusarium sp.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1