{"title":"硫酸盐还原菌和产氢醋酸菌的合成琼脂刺激废物活性污泥消化产生甲烷","authors":"Haokun Wu, Aijuan Zhou, Y. Duan, Zhihong Liu, Zhang-jie He, Wenzong Liu, Xiuping Yue","doi":"10.3390/fermentation10050243","DOIUrl":null,"url":null,"abstract":"Anaerobic digestion of waste-activated sludge (WAS) towards biogas recovery is constrained by the limited hydrolysis and inhibited acetogenesis steps that hinder subsequent energy recovery. This study employed Fe(VI)/S(IV) oxidation to enhance the WAS solubilization and coupled it with the syntrophic interaction of hydrogen-producing acetogen (HPA) and sulfate-reducing bacteria (SRB) to stimulate the successive procedure towards methane production. Results showed that the dosage ratio of HPA-SRB to WAS (H-S-W) with 1:1:50 outperformed with the highest methane production potential (11.63 ± 1.87 mL CH4/(g VSS·d). Meanwhile, the efficient and sequential process from acetogenesis to methanogenesis stimulated by HPA-SRB was evidenced by a significant decrease of 30.2% in the acetate concentration. The microbial community structure further manifested the crucial role of HPA-SRB with increased abundance of Desulfobulbus (2.07%), Syntrophomonas (1.24%) and Smithella (1.63%), which stimulated acetophilic methanogen boost with Methanobacterium dominating with 77.51% in H-S-W100. Furthermore, the positive syntrophic relationships among HPA-SRB and acetophilic methanogens towards methane production were confirmed via molecular ecological network and canonical correspondence analysis. This study highlighted the syntrophic cooperation of the mixed consortia of HPA and SRB on methane production based on Fe(VI)/S(IV) pretreatment and provided the theoretical and technical basis for the potential implementation of novel methanogenesis technology for WAS treatment.","PeriodicalId":12379,"journal":{"name":"Fermentation","volume":"19 75","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Syntrophic Jiont of Sulfate-Reducing Bacteria and Hydrogen-Producing Acetogen Stimulated Methane Production from Waste Activated Sludge Digestion\",\"authors\":\"Haokun Wu, Aijuan Zhou, Y. Duan, Zhihong Liu, Zhang-jie He, Wenzong Liu, Xiuping Yue\",\"doi\":\"10.3390/fermentation10050243\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Anaerobic digestion of waste-activated sludge (WAS) towards biogas recovery is constrained by the limited hydrolysis and inhibited acetogenesis steps that hinder subsequent energy recovery. This study employed Fe(VI)/S(IV) oxidation to enhance the WAS solubilization and coupled it with the syntrophic interaction of hydrogen-producing acetogen (HPA) and sulfate-reducing bacteria (SRB) to stimulate the successive procedure towards methane production. Results showed that the dosage ratio of HPA-SRB to WAS (H-S-W) with 1:1:50 outperformed with the highest methane production potential (11.63 ± 1.87 mL CH4/(g VSS·d). Meanwhile, the efficient and sequential process from acetogenesis to methanogenesis stimulated by HPA-SRB was evidenced by a significant decrease of 30.2% in the acetate concentration. The microbial community structure further manifested the crucial role of HPA-SRB with increased abundance of Desulfobulbus (2.07%), Syntrophomonas (1.24%) and Smithella (1.63%), which stimulated acetophilic methanogen boost with Methanobacterium dominating with 77.51% in H-S-W100. Furthermore, the positive syntrophic relationships among HPA-SRB and acetophilic methanogens towards methane production were confirmed via molecular ecological network and canonical correspondence analysis. This study highlighted the syntrophic cooperation of the mixed consortia of HPA and SRB on methane production based on Fe(VI)/S(IV) pretreatment and provided the theoretical and technical basis for the potential implementation of novel methanogenesis technology for WAS treatment.\",\"PeriodicalId\":12379,\"journal\":{\"name\":\"Fermentation\",\"volume\":\"19 75\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-05-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fermentation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/fermentation10050243\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fermentation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/fermentation10050243","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
为回收沼气而对废物活性污泥(WAS)进行的厌氧消化受到水解作用有限和产乙缩醛步骤受抑制的限制,这阻碍了后续的能量回收。本研究采用铁(VI)/硫(IV)氧化法提高 WAS 的溶解度,并将其与产氢醋酸菌(HPA)和硫酸盐还原菌(SRB)的综合作用结合起来,以刺激甲烷生产的连续过程。结果表明,HPA-SRB 与 WAS(H-S-W)的用量比为 1:1:50,甲烷生产潜力最大(11.63 ± 1.87 mL CH4/(g VSS-d))。同时,在 HPA-SRB 的刺激下,乙酸浓度显著下降了 30.2%,证明了从乙酸生成到甲烷生成的高效和连续过程。微生物群落结构进一步显示了 HPA-SRB 的关键作用,在 H-S-W100 中,Desulfobulbus(2.07%)、Syntrophomonas(1.24%)和 Smithella(1.63%)的丰度增加,刺激了嗜乙酰甲烷菌的繁殖,其中甲烷杆菌占 77.51%。此外,通过分子生态网络和典型对应分析,证实了 HPA-SRB 与嗜乙酰甲烷菌之间在甲烷生产方面的正向综合营养关系。该研究强调了基于 Fe(VI)/S(IV) 预处理的 HPA 和 SRB 混合菌群在产甲烷过程中的协同作用,为新型甲烷生成技术在 WAS 处理中的潜在应用提供了理论和技术基础。
Syntrophic Jiont of Sulfate-Reducing Bacteria and Hydrogen-Producing Acetogen Stimulated Methane Production from Waste Activated Sludge Digestion
Anaerobic digestion of waste-activated sludge (WAS) towards biogas recovery is constrained by the limited hydrolysis and inhibited acetogenesis steps that hinder subsequent energy recovery. This study employed Fe(VI)/S(IV) oxidation to enhance the WAS solubilization and coupled it with the syntrophic interaction of hydrogen-producing acetogen (HPA) and sulfate-reducing bacteria (SRB) to stimulate the successive procedure towards methane production. Results showed that the dosage ratio of HPA-SRB to WAS (H-S-W) with 1:1:50 outperformed with the highest methane production potential (11.63 ± 1.87 mL CH4/(g VSS·d). Meanwhile, the efficient and sequential process from acetogenesis to methanogenesis stimulated by HPA-SRB was evidenced by a significant decrease of 30.2% in the acetate concentration. The microbial community structure further manifested the crucial role of HPA-SRB with increased abundance of Desulfobulbus (2.07%), Syntrophomonas (1.24%) and Smithella (1.63%), which stimulated acetophilic methanogen boost with Methanobacterium dominating with 77.51% in H-S-W100. Furthermore, the positive syntrophic relationships among HPA-SRB and acetophilic methanogens towards methane production were confirmed via molecular ecological network and canonical correspondence analysis. This study highlighted the syntrophic cooperation of the mixed consortia of HPA and SRB on methane production based on Fe(VI)/S(IV) pretreatment and provided the theoretical and technical basis for the potential implementation of novel methanogenesis technology for WAS treatment.