评价作为免疫调节剂的豨莶草提取物在小鼠体内的吞噬指数和血液学参数

IF 0.5 Q4 EDUCATION, SCIENTIFIC DISCIPLINES Pharmacy Education Pub Date : 2024-05-01 DOI:10.46542/pe.2024.243.95100
T. Wahyuni, Widya Wasityastuti, D. Nugrahaningsih, Suratno Lulut Ratnoglik, Laura Yamani, Ahmad El-Shamy, A. Widyawaruyanti
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引用次数: 0

摘要

背景:据报道,Sida rhombifolia具有潜在的抗丙型肝炎病毒活性。据报道,植物的抗病毒活性还能调节免疫:本研究通过测试豨莶草提取物的免疫调节活性来进行:本研究评估了 HepG2 培养细胞的细胞活力,以确定细胞活力的 50%。免疫调节活性通过检测吞噬细胞指数值和血液学特征进行评估:结果表明,西达荷叶的 IC50 值为 32.5 μg/mL,而作为阴性对照的 CCl4 的 IC50 值为 16.68 μg/mL。此外,在水飞蓟 IC50 值为 ½ 和 ¼ 时,细胞存活率均为 100%,这表明水飞蓟提取物没有毒性作用。免疫调节试验发现,豨莶草提取物具有免疫增强作用。西达荷叶的吞噬细胞指数值高于标准药物(一种商用免疫增强剂产品)。此外,血液学评估显示,对血红蛋白、血细胞比容和红细胞没有负面影响,其数值与正常对照组和免疫刺激剂产品相比没有差异:结论:豨莶草提取物具有作为治疗肝炎的免疫调节剂的潜力。
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Evaluation of phagocytic index and haematological parameters of Sida rhombifolia extracts in mice as immunomodulator
Background: Sida rhombifolia was reported to have potential anti-hepatitis C virus activity. Anti-viral activities of plants were also reported to modulate immunity. Objective: This research was conducted by testing the immunomodulating activities of Sida rhombifolia extract. Method: This study evaluated cell viability in HepG2 culture cells to determine the 50% of cell viability. The Immunomodulatory activity was evaluated by examining the phagocytic index value and haematology profile. Result: The results showed that the IC50 Sida rhombifolia was 32.5 μg/mL, while the IC50 of CCl4 as negative control was 16.68 μg/mL. Moreover, 100% of cell viability was observed at ½ IC50 and ¼ IC50 of Sida rhombifolia, indicating no toxic effect from the extract. Immunomodulatory assay found that Sida rhombifolia extract demonstrated an immunostimulant effect. The phagocytic index value of Sida rhombifolia was higher than that of the standard drug (a commercial immunostimulant product). Moreover, the haematology evaluation demonstrated no negative effect on haemoglobin, hematocrit and erythrocyte, which showed no difference in values compared to the normal control and the immunostimulant product. Conclusion: Sida rhombifolia plant extract has the potential as an immunomodulator candidate for treating hepatitis.
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来源期刊
Pharmacy Education
Pharmacy Education EDUCATION, SCIENTIFIC DISCIPLINES-
CiteScore
0.80
自引率
20.00%
发文量
174
期刊介绍: Pharmacy Education journal provides a research, development and evaluation forum for communication between academic teachers, researchers and practitioners in professional and pharmacy education, with an emphasis on new and established teaching and learning methods, new curriculum and syllabus directions, educational outcomes, guidance on structuring courses and assessing achievement, and workforce development. It is a peer-reviewed online open access platform for the dissemination of new ideas in professional pharmacy education and workforce development. Pharmacy Education supports Open Access (OA): free, unrestricted online access to research outputs. Readers are able to access the Journal and individual published articles for free - there are no subscription fees or ''pay per view'' charges. Authors wishing to publish their work in Pharmacy Education do so without incurring any financial costs.
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