{"title":"胎儿窘迫对脐带血干细胞存活率和产量的影响--一项前瞻性比较研究","authors":"Bharathi Muthu, Prabhu Manivannan, Murali Subbaiah, Shreya Vanju, Abhishekh Basavarajegowda","doi":"10.1016/j.htct.2024.03.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><div>Different factors affect the quality and viability of cord blood stem cells, and therefore the efficacy of umbilical cord stem cell transplantation. Fetal distress is one factor affecting the quantity of CD34<sup>+</sup> cells in cord blood. This study was designed to compare the viability and yield of the umbilical cord blood stem cells of women who have undergone emergency lower segment caesarean section for fetal distress or for other causes.</div></div><div><h3>Materials and methods</h3><div>This cross-sectional analytical study was performed at a tertiary care hospital facility with a total sample size of 68: 34 participants had undergone emergency C-section for fetal distress, and 34 had undergone emergency C-section for other causes. Umbilical cord blood was collected <em>ex-utero</em> in a 350 mL bag with citrate-phosphate-dextrose solution with adenine. Three milliliter of blood were transferred to an ethylenediaminetetraacetic acid (EDTA) tube for cell counts and flow cytometry testing for CD34<sup>+</sup>. The chi-square test was used to compare the total mononuclear cell, CD34<sup>+</sup>, and viability between the groups.</div></div><div><h3>Results</h3><div>The CD34<sup>+</sup> count [mean 4.9 versus 1.1 (× 10<sup>6</sup> cells/unit)] and total nucleated cell count [mean 14.2 versus 7.5 (× 10<sup>8</sup>/unit)] were significantly higher in cord blood units collected from women who delivered by C-section for fetal distress (<em>p</em>-value <0.05). However, the volume of umbilical cord stem cells and viability of stem cells did not vary significantly based on the presence or absence of fetal distress (<em>p</em>-value >0.05).</div></div><div><h3>Conclusion</h3><div>The current study shows that umbilical cord blood collected during fetal distress has a significantly higher content of stem cells and total nucleated cells than the non-fetal distress group</div></div>","PeriodicalId":12958,"journal":{"name":"Hematology, Transfusion and Cell Therapy","volume":"46 ","pages":"Pages S157-S162"},"PeriodicalIF":1.5000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of fetal distress on viability and yield of umbilical cord blood stem cells–a prospective comparative study\",\"authors\":\"Bharathi Muthu, Prabhu Manivannan, Murali Subbaiah, Shreya Vanju, Abhishekh Basavarajegowda\",\"doi\":\"10.1016/j.htct.2024.03.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><div>Different factors affect the quality and viability of cord blood stem cells, and therefore the efficacy of umbilical cord stem cell transplantation. Fetal distress is one factor affecting the quantity of CD34<sup>+</sup> cells in cord blood. This study was designed to compare the viability and yield of the umbilical cord blood stem cells of women who have undergone emergency lower segment caesarean section for fetal distress or for other causes.</div></div><div><h3>Materials and methods</h3><div>This cross-sectional analytical study was performed at a tertiary care hospital facility with a total sample size of 68: 34 participants had undergone emergency C-section for fetal distress, and 34 had undergone emergency C-section for other causes. Umbilical cord blood was collected <em>ex-utero</em> in a 350 mL bag with citrate-phosphate-dextrose solution with adenine. Three milliliter of blood were transferred to an ethylenediaminetetraacetic acid (EDTA) tube for cell counts and flow cytometry testing for CD34<sup>+</sup>. The chi-square test was used to compare the total mononuclear cell, CD34<sup>+</sup>, and viability between the groups.</div></div><div><h3>Results</h3><div>The CD34<sup>+</sup> count [mean 4.9 versus 1.1 (× 10<sup>6</sup> cells/unit)] and total nucleated cell count [mean 14.2 versus 7.5 (× 10<sup>8</sup>/unit)] were significantly higher in cord blood units collected from women who delivered by C-section for fetal distress (<em>p</em>-value <0.05). However, the volume of umbilical cord stem cells and viability of stem cells did not vary significantly based on the presence or absence of fetal distress (<em>p</em>-value >0.05).</div></div><div><h3>Conclusion</h3><div>The current study shows that umbilical cord blood collected during fetal distress has a significantly higher content of stem cells and total nucleated cells than the non-fetal distress group</div></div>\",\"PeriodicalId\":12958,\"journal\":{\"name\":\"Hematology, Transfusion and Cell Therapy\",\"volume\":\"46 \",\"pages\":\"Pages S157-S162\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hematology, Transfusion and Cell Therapy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2531137924002219\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/5/6 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"HEMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hematology, Transfusion and Cell Therapy","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2531137924002219","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/6 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"HEMATOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
不同的因素影响脐带血干细胞的质量和活力，从而影响脐带血干细胞移植的疗效。胎儿窘迫是影响脐带血中CD34+细胞数量的一个因素。本研究旨在比较因胎儿窘迫或其他原因进行紧急下段剖宫产的妇女脐带血干细胞的活力和产量。材料和方法本横断面分析研究在一家三级护理医院进行，总样本量为68人：34名参与者因胎儿窘迫进行了紧急剖腹产，34名参与者因其他原因进行了紧急剖腹产。脐带血在子宫外用柠檬酸-磷酸盐-葡萄糖腺嘌呤溶液350 mL袋收集。将3毫升血液转移到乙二胺四乙酸（EDTA）管中进行细胞计数和流式细胞术检测CD34+。采用卡方检验比较各组间总单核细胞、CD34+和活力。结果脐带血CD34+计数[平均4.9 vs 1.1 （× 106细胞/单位）]和总有核细胞计数[平均14.2 vs 7.5 （× 108细胞/单位）]在因胎儿窘迫剖腹产分娩的妇女中显著高于脐带血单位（p值<；0.05）。然而，脐带干细胞的体积和干细胞的活力并没有因胎儿窘迫的存在或不存在而显著变化（p值>；0.05）。结论胎儿窘迫时采集的脐带血干细胞和总有核细胞含量明显高于非胎儿窘迫组
Effect of fetal distress on viability and yield of umbilical cord blood stem cells–a prospective comparative study
Introduction
Different factors affect the quality and viability of cord blood stem cells, and therefore the efficacy of umbilical cord stem cell transplantation. Fetal distress is one factor affecting the quantity of CD34+ cells in cord blood. This study was designed to compare the viability and yield of the umbilical cord blood stem cells of women who have undergone emergency lower segment caesarean section for fetal distress or for other causes.
Materials and methods
This cross-sectional analytical study was performed at a tertiary care hospital facility with a total sample size of 68: 34 participants had undergone emergency C-section for fetal distress, and 34 had undergone emergency C-section for other causes. Umbilical cord blood was collected ex-utero in a 350 mL bag with citrate-phosphate-dextrose solution with adenine. Three milliliter of blood were transferred to an ethylenediaminetetraacetic acid (EDTA) tube for cell counts and flow cytometry testing for CD34+. The chi-square test was used to compare the total mononuclear cell, CD34+, and viability between the groups.
Results
The CD34+ count [mean 4.9 versus 1.1 (× 106 cells/unit)] and total nucleated cell count [mean 14.2 versus 7.5 (× 108/unit)] were significantly higher in cord blood units collected from women who delivered by C-section for fetal distress (p-value <0.05). However, the volume of umbilical cord stem cells and viability of stem cells did not vary significantly based on the presence or absence of fetal distress (p-value >0.05).
Conclusion
The current study shows that umbilical cord blood collected during fetal distress has a significantly higher content of stem cells and total nucleated cells than the non-fetal distress group
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