SigF1 依赖性 pilA1 基因启动子的结构和蓝藻细长球藻 PCC 7942 光激活反应的特征。

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General and Applied Microbiology Pub Date : 2024-11-06 Epub Date: 2024-05-20 DOI:10.2323/jgam.2024.05.002
Ying Luo, Hitomi Imamitsu, Tatsuhiro Tsurumaki, Kan Tanaka
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引用次数: 0

摘要

在进行含氧光合作用的蓝藻中,替代性 sigma 因子可在转录起始步骤的环境适应中发挥关键作用。在这里,我们发现在拉长的 Synechococcus PCC 7942 中,编码 IV 型柔毛蛋白的 pilA1 基因的转录依赖于第 3 组 sigma 因子之一 SigF1。我们分析了启动子序列决定因素,并在此提出转录起始位点上游的-10 和-35 框对转录至关重要。有趣的是,虽然光照会激活 pilA1 启动子,但在光照之前的黑暗条件下,含有 SigF1 的 RNA 聚合酶已经位于启动子区域。这有力地表明,在转录启动过程中,RNA聚合酶的招募是启动子被光激活的先决条件。
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Structure of the SigF1-dependent pilA1 gene promoter and characterization of the light-activated response in the cyanobacterium Synechococcus elongatus PCC 7942.

In cyanobacteria that perform oxygenic photosynthesis, alternative sigma factors can play critical roles in environmental acclimation at the transcriptional initiation step. Here, we found in Synechococcus elongatus PCC 7942 that transcription of the pilA1 gene, encoding the type IV pilin, is dependent on one of the group 3 sigma factors, SigF1. We analyzed the promoter sequence determinants and proposed herein that the -10 and -35 boxes upstream of the transcriptional start site are critical for transcription. Interestingly, while the pilA1 promoter is activated by illumination, RNA polymerase containing SigF1 is already located on the promoter region under dark conditions, prior to illumination. This strongly suggests that promoter activation by light follows the recruitment of RNA polymerase during transcriptional initiation.

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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
期刊最新文献
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