Ariel Berl, Ofir Shir-Az, Shelly Tartakover-Matalon, Vladimir Kravtsov, Alexander Golberg, Mrinal Kashyap, Avshalom Shalom
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Sutures were passed through the observed tumour or healthy skin margins and were examined for DNA material and cells by cytological analysis, cell culture and characterization, and DNA analysis.</p><p><strong>Results: </strong>Twenty-two patients and 148 sutures were included. DNA quantification showed DNA material on all sutures, with no significant difference between braided and monofilament sutures. Cytological analysis showed that all slides prepared from cell blocks contained normal squamous and atypical cells. Cell culture and characterization showed viable cells adhering to the sutures under direct light microscopy. Cell cultures showed rapid proliferation of epithelial cells from squamous cell carcinoma specimens.</p><p><strong>Conclusion: </strong>Suture materials carry DNA material and cells, including malignant cells of cutaneous origin and may seed them at distant sites.</p>","PeriodicalId":15403,"journal":{"name":"Journal of Cutaneous Medicine and Surgery","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transmission of Genetic Material and Malignant Cells of Cutaneous Origin via Surgical Sutures.\",\"authors\":\"Ariel Berl, Ofir Shir-Az, Shelly Tartakover-Matalon, Vladimir Kravtsov, Alexander Golberg, Mrinal Kashyap, Avshalom Shalom\",\"doi\":\"10.1177/12034754241253189\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Seeding of skin cancer cells following diagnostic or therapeutic surgical procedures can occur and might cause local recurrences. As current preferred therapy for skin malignancy is surgical excision, seeding of tumour cells by manipulating malignant tissue or suturing can be another factor leading to recurrences.</p><p><strong>Objective: </strong>To evaluate whether genetic material and malignant cells adhere to standard suture materials.</p><p><strong>Methods: </strong>This prospective study included patients who underwent excision of skin lesions. Monofilament and braided sutures were examined. Sutures were passed through the observed tumour or healthy skin margins and were examined for DNA material and cells by cytological analysis, cell culture and characterization, and DNA analysis.</p><p><strong>Results: </strong>Twenty-two patients and 148 sutures were included. DNA quantification showed DNA material on all sutures, with no significant difference between braided and monofilament sutures. Cytological analysis showed that all slides prepared from cell blocks contained normal squamous and atypical cells. Cell culture and characterization showed viable cells adhering to the sutures under direct light microscopy. Cell cultures showed rapid proliferation of epithelial cells from squamous cell carcinoma specimens.</p><p><strong>Conclusion: </strong>Suture materials carry DNA material and cells, including malignant cells of cutaneous origin and may seed them at distant sites.</p>\",\"PeriodicalId\":15403,\"journal\":{\"name\":\"Journal of Cutaneous Medicine and Surgery\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Cutaneous Medicine and Surgery\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1177/12034754241253189\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/5/20 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"DERMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cutaneous Medicine and Surgery","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/12034754241253189","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/20 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"DERMATOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
背景:皮肤癌细胞在诊断或治疗手术后可能会播种,并可能导致局部复发。由于目前治疗皮肤恶性肿瘤的首选方法是手术切除,因此通过操作恶性组织或缝合而使肿瘤细胞播种可能是导致复发的另一个因素:评估遗传物质和恶性细胞是否附着在标准缝合材料上:这项前瞻性研究包括接受皮肤病变切除术的患者。对单丝缝合线和编织缝合线进行了检查。缝合线穿过观察到的肿瘤或健康皮肤边缘,并通过细胞学分析、细胞培养和特征描述以及 DNA 分析检测 DNA 物质和细胞:结果:共纳入 22 名患者和 148 条缝合线。DNA 定量分析显示,所有缝合线上都有 DNA 物质,编织缝合线和单丝缝合线之间无明显差异。细胞学分析表明,从细胞块中制备的所有切片都含有正常的鳞状细胞和非典型细胞。细胞培养和特征描述显示,在直接光学显微镜下,缝合线上附着有活力的细胞。细胞培养显示,鳞状细胞癌标本的上皮细胞迅速增殖:结论:缝合材料携带 DNA 物质和细胞,包括皮肤来源的恶性细胞,并可能将其播种到远处的部位。
Transmission of Genetic Material and Malignant Cells of Cutaneous Origin via Surgical Sutures.
Background: Seeding of skin cancer cells following diagnostic or therapeutic surgical procedures can occur and might cause local recurrences. As current preferred therapy for skin malignancy is surgical excision, seeding of tumour cells by manipulating malignant tissue or suturing can be another factor leading to recurrences.
Objective: To evaluate whether genetic material and malignant cells adhere to standard suture materials.
Methods: This prospective study included patients who underwent excision of skin lesions. Monofilament and braided sutures were examined. Sutures were passed through the observed tumour or healthy skin margins and were examined for DNA material and cells by cytological analysis, cell culture and characterization, and DNA analysis.
Results: Twenty-two patients and 148 sutures were included. DNA quantification showed DNA material on all sutures, with no significant difference between braided and monofilament sutures. Cytological analysis showed that all slides prepared from cell blocks contained normal squamous and atypical cells. Cell culture and characterization showed viable cells adhering to the sutures under direct light microscopy. Cell cultures showed rapid proliferation of epithelial cells from squamous cell carcinoma specimens.
Conclusion: Suture materials carry DNA material and cells, including malignant cells of cutaneous origin and may seed them at distant sites.
期刊介绍:
Journal of Cutaneous Medicine and Surgery (JCMS) aims to reflect the state of the art in cutaneous biology and dermatology by providing original scientific writings, as well as a complete critical review of the dermatology literature for clinicians, trainees, and academicians. JCMS endeavours to bring readers cutting edge dermatologic information in two distinct formats. Part of each issue features scholarly research and articles on issues of basic and applied science, insightful case reports, comprehensive continuing medical education, and in depth reviews, all of which provide theoretical framework for practitioners to make sound practical decisions. The evolving field of dermatology is highlighted through these articles. In addition, part of each issue is dedicated to making the most important developments in dermatology easily accessible to the clinician by presenting well-chosen, well-written, and highly organized information in a format that is interesting, clearly presented, and useful to patient care.