在大肠杆菌中生物生产甲基化苯丙烯和异丁香酚

IF 3.7 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Metabolic Engineering Communications Pub Date : 2024-05-15 DOI:10.1016/j.mec.2024.e00237
Jeremy Chua, Erik K.R. Hanko, Andrew Yiakoumetti, Ruth A. Stoney, Jakub Chromy, Kris Niño G. Valdehuesa, Katherine A. Hollywood, Cunyu Yan, Eriko Takano, Rainer Breitling
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引用次数: 0

摘要

苯丙烯是一类由多种植物合成的天然产品,在香料和香精工业中大有可为。已经进行了许多体外研究,以阐明负责生产这些挥发性化合物的酶的特性。然而,在微生物细胞工厂中体内生产苯丙烯的研究却很少。在本研究中,我们改造了大肠杆菌,使其能够从各自的苯丙氨酸前体中生产甲基茶维素、甲基丁香酚和异丁香酚。我们通过扩展和修改以前优化的异源途径来实现茶维醇和丁香酚的生物合成。我们探索了六种依赖于 S-腺苷-l-蛋氨酸(SAM)的 O-甲基转移酶分别从茶维醇和丁香酚生成甲基茶维醇和甲基丁香酚的潜力。此外,我们还研究了两种异丁香酚合成酶,以从针叶乙酸酯中生产异丁香酚。本研究中表现最好的菌株在喂食适当的苯丙氨酸底物后,能达到 13 mg L-1 甲基茶维醇、59 mg L-1 甲基丁香酚和 361 mg L-1 异丁香酚的滴度。通过补充蛋氨酸促进 SAM 循环,我们能够将甲基丁香酚滴度进一步提高到 117 mg L-1。此外,我们还报告了由 l-酪氨酸通过分别涉及六个和八个酶步骤的途径生物合成甲基茶酚和甲基丁香酚的情况。
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Bioproduction of methylated phenylpropenes and isoeugenol in Escherichia coli

Phenylpropenes are a class of natural products that are synthesised by a vast range of plant species and hold considerable promise in the flavour and fragrance industries. Many in vitro studies have been carried out to elucidate and characterise the enzymes responsible for the production of these volatile compounds. However, there is a scarcity of studies demonstrating the in vivo production of phenylpropenes in microbial cell factories. In this study, we engineered Escherichia coli to produce methylchavicol, methyleugenol and isoeugenol from their respective phenylacrylic acid precursors. We achieved this by extending and modifying a previously optimised heterologous pathway for the biosynthesis of chavicol and eugenol. We explored the potential of six S-adenosyl l-methionine (SAM)-dependent O-methyltransferases to produce methylchavicol and methyleugenol from chavicol and eugenol, respectively. Additionally, we examined two isoeugenol synthases for the production of isoeugenol from coniferyl acetate. The best-performing strains in this study were able to achieve titres of 13 mg L−1 methylchavicol, 59 mg L−1 methyleugenol and 361 mg L−1 isoeugenol after feeding with their appropriate phenylacrylic acid substrates. We were able to further increase the methyleugenol titre to 117 mg L−1 by supplementation with methionine to facilitate SAM recycling. Moreover, we report the biosynthesis of methylchavicol and methyleugenol from l-tyrosine through pathways involving six and eight enzymatic steps, respectively.

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来源期刊
Metabolic Engineering Communications
Metabolic Engineering Communications Medicine-Endocrinology, Diabetes and Metabolism
CiteScore
13.30
自引率
1.90%
发文量
22
审稿时长
18 weeks
期刊介绍: Metabolic Engineering Communications, a companion title to Metabolic Engineering (MBE), is devoted to publishing original research in the areas of metabolic engineering, synthetic biology, computational biology and systems biology for problems related to metabolism and the engineering of metabolism for the production of fuels, chemicals, and pharmaceuticals. The journal will carry articles on the design, construction, and analysis of biological systems ranging from pathway components to biological complexes and genomes (including genomic, analytical and bioinformatics methods) in suitable host cells to allow them to produce novel compounds of industrial and medical interest. Demonstrations of regulatory designs and synthetic circuits that alter the performance of biochemical pathways and cellular processes will also be presented. Metabolic Engineering Communications complements MBE by publishing articles that are either shorter than those published in the full journal, or which describe key elements of larger metabolic engineering efforts.
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