绿斑河豚的特定嗅觉神经元能检测到无毒河豚毒素类似物--5,6,11-三脱氧河豚毒素的气味。

IF 2.8 4区 心理学 Q1 BEHAVIORAL SCIENCES Chemical Senses Pub Date : 2024-01-01 DOI:10.1093/chemse/bjae021
Takehisa Suzuki, Ryota Nakahigashi, Masaatsu Adachi, Toshio Nishikawa, Hideki Abe
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引用次数: 0

摘要

毒河豚通过捕食含河豚毒素的生物并利用河豚毒素抵御天敌,从而积累了河豚毒素(TTX),这是一种众所周知的神经毒素。我们之前的研究表明,毒河豚会被 5,6,11-三脱氧河豚毒素(TDT)吸引,TDT 是一种无毒的 TTX 类似物,在毒河豚及其猎物体内与 TTX 同时积累。此外,针对神经元活动标记的免疫组织化学活动标记表明,TDT 能激活绿斑河豚的隐窝嗅觉神经元(OSN)。然而,单个隐窝嗅觉神经元能否对TDT产生生理反应仍有待确定。通过电穿孔在OSNs中表达GCaMP6s,我们成功地鉴定出了一组不同的椭圆形OSNs,当暴露于绿斑河豚的TDT时,这些OSNs会表现出特定的钙反应。这些椭圆形 OSNs 对氨基酸(AAs)没有反应,而氨基酸是远洋鱼类的食物气味线索。此外,嗅上皮中对 TDT 敏感的椭圆形 OSNs 的形态和表面定位与隐窝 OSNs 非常相似。这些发现表明,绿斑河豚的隐窝 OSNs 能特异性地检测到 TDT。TDT气味可能是一种趋化吸引剂,用于寻找同种有毒河鲀,并喂食含TTX的生物以有效解毒。
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The odor of a nontoxic tetrodotoxin analog, 5,6,11-trideoxytetrodotoxin, is detected by specific olfactory sensory neurons of the green spotted puffers.

Toxic puffers accumulate tetrodotoxin (TTX), a well-known neurotoxin, by feeding on TTX-bearing organisms and using it to defend themselves from predators. Our previous studies have demonstrated that toxic puffers are attracted to 5,6,11-trideoxytetrodotoxin (TDT), a nontoxic TTX analog that is simultaneously accumulated with TTX in toxic puffers and their prey. In addition, activity labeling using immunohistochemistry targeting neuronal activity marker suggests that TDT activates crypt olfactory sensory neurons (OSN) of the green spotted puffer. However, it remains to be determined whether individual crypt OSNs can physiologically respond to TDT. By employing electroporation to express GCaMP6s in OSNs, we successfully identified a distinct group of oval OSNs that exhibited a specific calcium response when exposed to TDT in green spotted puffers. These oval OSNs showed no response to amino acids (AAs), which serve as food odor cues for teleosts. Furthermore, oval morphology and surface positioning of TDT-sensitive OSNs in the olfactory epithelium closely resemble that of crypt OSNs. These findings further substantiate that TDT is specifically detected by crypt OSNs in green spotted puffer. The TDT odor may act as a chemoattractant for finding conspecific toxic puffers and for feeding TTX-bearing organisms for effective toxification.

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来源期刊
Chemical Senses
Chemical Senses 医学-行为科学
CiteScore
8.60
自引率
2.90%
发文量
25
审稿时长
1 months
期刊介绍: Chemical Senses publishes original research and review papers on all aspects of chemoreception in both humans and animals. An important part of the journal''s coverage is devoted to techniques and the development and application of new methods for investigating chemoreception and chemosensory structures.
期刊最新文献
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