Atte Sjövall, Ella Mustanoja, Annina Lyyski, Petri Auvinen, Juha Silvola, Antti Aarnisalo, Anu Pätäri-Sampo, Anu Laulajainen-Hongisto
{"title":"外耳道微生物组:长期使用助听器后的变化","authors":"Atte Sjövall, Ella Mustanoja, Annina Lyyski, Petri Auvinen, Juha Silvola, Antti Aarnisalo, Anu Pätäri-Sampo, Anu Laulajainen-Hongisto","doi":"10.1097/MAO.0000000000004198","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the microbial changes of long-term hearing aid use culture independently.</p><p><strong>Study design: </strong>Cross-sectional study.</p><p><strong>Patients: </strong>Fifty long-term hearing aid users and 80 volunteer controls with asymptomatic ears.</p><p><strong>Intervention: </strong>External auditory canal (EAC) sampling with DNA-free swabs.</p><p><strong>Main outcome measures: </strong>Microbial communities in the samples were investigated with amplicon sequencing of the 16S rRNA gene.</p><p><strong>Results: </strong>The final analysis contained 48 hearing aid users, 59 controls. Twenty-four samples were excluded because of low sequence count, recent use of antimicrobials and/or corticosteroids, recent cold, or missing health status. The groups showed significant differences in bacterial diversity (beta div., p = 0.011), and hearing aid users showed lower species richness than the control group (alpha div., p < 0.01). The most frequent findings in both groups were Staphylococcus auricularis , Alloiococcus otitis , Cutibacterium acnes , Corynebacterium otitidis , and Staphylococcus unclassified sp. Hearing aid users' samples presented more Corynebacterium tuberculostearicum than the control samples. Common EAC pathogens, such as Staphylococcus aureus or Pseudomonas aeruginosa were rare.</p><p><strong>Conclusion: </strong>Long-term hearing aid use lowers bacterial diversity and modulates the EAC microbiome. The changes mostly affect commensals. Lowered diversity may predispose individuals to EAC conditions and needs more research.</p>","PeriodicalId":19732,"journal":{"name":"Otology & Neurotology","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Microbiome of the External Auditory Canal: Changes After Long-Term Hearing Aid Use.\",\"authors\":\"Atte Sjövall, Ella Mustanoja, Annina Lyyski, Petri Auvinen, Juha Silvola, Antti Aarnisalo, Anu Pätäri-Sampo, Anu Laulajainen-Hongisto\",\"doi\":\"10.1097/MAO.0000000000004198\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the microbial changes of long-term hearing aid use culture independently.</p><p><strong>Study design: </strong>Cross-sectional study.</p><p><strong>Patients: </strong>Fifty long-term hearing aid users and 80 volunteer controls with asymptomatic ears.</p><p><strong>Intervention: </strong>External auditory canal (EAC) sampling with DNA-free swabs.</p><p><strong>Main outcome measures: </strong>Microbial communities in the samples were investigated with amplicon sequencing of the 16S rRNA gene.</p><p><strong>Results: </strong>The final analysis contained 48 hearing aid users, 59 controls. Twenty-four samples were excluded because of low sequence count, recent use of antimicrobials and/or corticosteroids, recent cold, or missing health status. The groups showed significant differences in bacterial diversity (beta div., p = 0.011), and hearing aid users showed lower species richness than the control group (alpha div., p < 0.01). The most frequent findings in both groups were Staphylococcus auricularis , Alloiococcus otitis , Cutibacterium acnes , Corynebacterium otitidis , and Staphylococcus unclassified sp. Hearing aid users' samples presented more Corynebacterium tuberculostearicum than the control samples. Common EAC pathogens, such as Staphylococcus aureus or Pseudomonas aeruginosa were rare.</p><p><strong>Conclusion: </strong>Long-term hearing aid use lowers bacterial diversity and modulates the EAC microbiome. The changes mostly affect commensals. Lowered diversity may predispose individuals to EAC conditions and needs more research.</p>\",\"PeriodicalId\":19732,\"journal\":{\"name\":\"Otology & Neurotology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Otology & Neurotology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1097/MAO.0000000000004198\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/5/21 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"CLINICAL NEUROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Otology & Neurotology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1097/MAO.0000000000004198","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/21 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"CLINICAL NEUROLOGY","Score":null,"Total":0}
Microbiome of the External Auditory Canal: Changes After Long-Term Hearing Aid Use.
Objective: To investigate the microbial changes of long-term hearing aid use culture independently.
Study design: Cross-sectional study.
Patients: Fifty long-term hearing aid users and 80 volunteer controls with asymptomatic ears.
Intervention: External auditory canal (EAC) sampling with DNA-free swabs.
Main outcome measures: Microbial communities in the samples were investigated with amplicon sequencing of the 16S rRNA gene.
Results: The final analysis contained 48 hearing aid users, 59 controls. Twenty-four samples were excluded because of low sequence count, recent use of antimicrobials and/or corticosteroids, recent cold, or missing health status. The groups showed significant differences in bacterial diversity (beta div., p = 0.011), and hearing aid users showed lower species richness than the control group (alpha div., p < 0.01). The most frequent findings in both groups were Staphylococcus auricularis , Alloiococcus otitis , Cutibacterium acnes , Corynebacterium otitidis , and Staphylococcus unclassified sp. Hearing aid users' samples presented more Corynebacterium tuberculostearicum than the control samples. Common EAC pathogens, such as Staphylococcus aureus or Pseudomonas aeruginosa were rare.
Conclusion: Long-term hearing aid use lowers bacterial diversity and modulates the EAC microbiome. The changes mostly affect commensals. Lowered diversity may predispose individuals to EAC conditions and needs more research.
期刊介绍:
Otology & Neurotology publishes original articles relating to both clinical and basic science aspects of otology, neurotology, and cranial base surgery. As the foremost journal in its field, it has become the favored place for publishing the best of new science relating to the human ear and its diseases. The broadly international character of its contributing authors, editorial board, and readership provides the Journal its decidedly global perspective.