Nora M El Naggar, Riham M Shawky, Fathy M E Serry, Mohamed Emara
{"title":"肺炎克雷伯菌分离株中同时携带 blaNDM 和 blaOXA-48 类基因的 rmpA 基因的流行率增加。","authors":"Nora M El Naggar, Riham M Shawky, Fathy M E Serry, Mohamed Emara","doi":"10.1089/mdr.2023.0296","DOIUrl":null,"url":null,"abstract":"<p><p>The emergence of carbapenemase-producing <i>Klebsiella pneumoniae</i> poses a substantial risk to public health. It is essential to comprehend the influence of carbapenemase on the virulence characteristics of <i>K. pneumoniae</i> in order to devise successful strategies for combating these infections. In this study, we explored the distribution disparity of virulence determinants between carbapenemase-producing (CP-Kp, <i>n</i> = 52) and carbapenemase-nonproducing (CN-Kp, <i>n</i> = 43) isolates. The presence of carbapenemases was detected via the modified carbapenem inactivation method and confirmed by PCR. The New Delhi metallo-β-lactamase (<i>bla</i><sub>NDM</sub>) and Oxacillinase-48-like (<i>bla</i><sub>OXA-48-like</sub>) genes were the most prevalent (94.23% and 76.92%, respectively) in CP-Kp isolates. Coexistence of <i>bla</i><sub>NDM</sub> and <i>bla</i><sub>OXA-48-like</sub> was observed in 71.15% of isolates, whereas 5.77% coharbored <i>bla</i><sub>NDM</sub> and <i>bla</i><sub>KPC</sub>. PCR analysis revealed the presence of several virulence genes, including adhesins (<i>fimH</i>, 92.63%, <i>mrkD</i>, 97.89%), capsule-associated virulence (<i>uge</i>, 90.53%), the K2 capsule serotype (<i>k2</i>, 6.32%), the iron acquisition system (<i>kfu</i>, 23.16%), and the regulator of mucoid phenotype (<i>rmpA</i>, 28.42%). A significantly higher prevalence of <i>rmpA</i> was detected in the CP-Kp compared with the CN-Kp (24/52 vs. 3/43, <i>p</i> < 0.0001), indicating a potential association between <i>rmpA</i> and carbapenemase acquisition. In addition, the majority of <i>rmpA</i> (22/24) positive isolates in the CP-Kp isolates coharbored <i>bla</i><sub>NDM</sub> and either <i>bla</i><sub>OXA-48-like</sub> or <i>bla</i><sub>KPC</sub>.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"317-324"},"PeriodicalIF":2.3000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Increased Prevalence of <i>rmpA</i> Gene in <i>Klebsiella pneumoniae</i> Isolates Coharboring <i>bla</i><sub>NDM</sub> and <i>bla</i><sub>OXA-48-like</sub> Genes.\",\"authors\":\"Nora M El Naggar, Riham M Shawky, Fathy M E Serry, Mohamed Emara\",\"doi\":\"10.1089/mdr.2023.0296\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The emergence of carbapenemase-producing <i>Klebsiella pneumoniae</i> poses a substantial risk to public health. It is essential to comprehend the influence of carbapenemase on the virulence characteristics of <i>K. pneumoniae</i> in order to devise successful strategies for combating these infections. In this study, we explored the distribution disparity of virulence determinants between carbapenemase-producing (CP-Kp, <i>n</i> = 52) and carbapenemase-nonproducing (CN-Kp, <i>n</i> = 43) isolates. The presence of carbapenemases was detected via the modified carbapenem inactivation method and confirmed by PCR. The New Delhi metallo-β-lactamase (<i>bla</i><sub>NDM</sub>) and Oxacillinase-48-like (<i>bla</i><sub>OXA-48-like</sub>) genes were the most prevalent (94.23% and 76.92%, respectively) in CP-Kp isolates. Coexistence of <i>bla</i><sub>NDM</sub> and <i>bla</i><sub>OXA-48-like</sub> was observed in 71.15% of isolates, whereas 5.77% coharbored <i>bla</i><sub>NDM</sub> and <i>bla</i><sub>KPC</sub>. PCR analysis revealed the presence of several virulence genes, including adhesins (<i>fimH</i>, 92.63%, <i>mrkD</i>, 97.89%), capsule-associated virulence (<i>uge</i>, 90.53%), the K2 capsule serotype (<i>k2</i>, 6.32%), the iron acquisition system (<i>kfu</i>, 23.16%), and the regulator of mucoid phenotype (<i>rmpA</i>, 28.42%). A significantly higher prevalence of <i>rmpA</i> was detected in the CP-Kp compared with the CN-Kp (24/52 vs. 3/43, <i>p</i> < 0.0001), indicating a potential association between <i>rmpA</i> and carbapenemase acquisition. In addition, the majority of <i>rmpA</i> (22/24) positive isolates in the CP-Kp isolates coharbored <i>bla</i><sub>NDM</sub> and either <i>bla</i><sub>OXA-48-like</sub> or <i>bla</i><sub>KPC</sub>.</p>\",\"PeriodicalId\":18701,\"journal\":{\"name\":\"Microbial drug resistance\",\"volume\":\" \",\"pages\":\"317-324\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbial drug resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1089/mdr.2023.0296\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/5/21 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial drug resistance","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1089/mdr.2023.0296","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/21 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
The Increased Prevalence of rmpA Gene in Klebsiella pneumoniae Isolates Coharboring blaNDM and blaOXA-48-like Genes.
The emergence of carbapenemase-producing Klebsiella pneumoniae poses a substantial risk to public health. It is essential to comprehend the influence of carbapenemase on the virulence characteristics of K. pneumoniae in order to devise successful strategies for combating these infections. In this study, we explored the distribution disparity of virulence determinants between carbapenemase-producing (CP-Kp, n = 52) and carbapenemase-nonproducing (CN-Kp, n = 43) isolates. The presence of carbapenemases was detected via the modified carbapenem inactivation method and confirmed by PCR. The New Delhi metallo-β-lactamase (blaNDM) and Oxacillinase-48-like (blaOXA-48-like) genes were the most prevalent (94.23% and 76.92%, respectively) in CP-Kp isolates. Coexistence of blaNDM and blaOXA-48-like was observed in 71.15% of isolates, whereas 5.77% coharbored blaNDM and blaKPC. PCR analysis revealed the presence of several virulence genes, including adhesins (fimH, 92.63%, mrkD, 97.89%), capsule-associated virulence (uge, 90.53%), the K2 capsule serotype (k2, 6.32%), the iron acquisition system (kfu, 23.16%), and the regulator of mucoid phenotype (rmpA, 28.42%). A significantly higher prevalence of rmpA was detected in the CP-Kp compared with the CN-Kp (24/52 vs. 3/43, p < 0.0001), indicating a potential association between rmpA and carbapenemase acquisition. In addition, the majority of rmpA (22/24) positive isolates in the CP-Kp isolates coharbored blaNDM and either blaOXA-48-like or blaKPC.
期刊介绍：
Microbial Drug Resistance (MDR) is an international, peer-reviewed journal that covers the global spread and threat of multi-drug resistant clones of major pathogens that are widely documented in hospitals and the scientific community. The Journal addresses the serious challenges of trying to decipher the molecular mechanisms of drug resistance. MDR provides a multidisciplinary forum for peer-reviewed original publications as well as topical reviews and special reports.
MDR coverage includes:
Molecular biology of resistance mechanisms
Virulence genes and disease
Molecular epidemiology
Drug design
Infection control.
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