{"title":"筛查伊朗西北部牛、绵羊和山羊中分离出的耐甲氧西林金黄色葡萄球菌的万古霉素抗性相关基因。","authors":"Leila Maleki, Amir Tukmechi","doi":"10.30466/vrf.2023.2003964.3902","DOIUrl":null,"url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> is an important pathogen causing a wide range of diseases in both humans and animals. The aim of this research was to screen the vancomycin resistance-associated genes in methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) isolates from animals. A total of 400 nasal swab samples were collected from cattle, goats and sheep between February and August 2022 from both industrial and traditional livestock farms in West Azerbaijan province, Iran. Then, nasal swabs were cultured on mannitol salt agar and molecular analysis was performed after bacteriological examination to confirm the presence of <i>S. aureus</i>. The <i>MecA</i> gene was used to detect MRSA isolates, and two important vancomycin resistance-associated genes, namely <i>vanA</i> and <i>vanB</i>, were searched in the isolates. Out of 400 nasal swabs, 69 samples had <i>S. aureus</i>; of which seven isolates were resistant against methicillin. No vancomycin resistance-associated genes were detected in the MRSA isolates. Based on these findings, vancomycin could be used to treat infections caused by this bacterium<i>.</i></p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102584/pdf/","citationCount":"0","resultStr":"{\"title\":\"Screening of vancomycin resistance-associated genes in methicillin-resistant <i>Staphylococcus aureus</i> isolates from cattle, sheep and goats in northwestern Iran.\",\"authors\":\"Leila Maleki, Amir Tukmechi\",\"doi\":\"10.30466/vrf.2023.2003964.3902\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Staphylococcus aureus</i> is an important pathogen causing a wide range of diseases in both humans and animals. The aim of this research was to screen the vancomycin resistance-associated genes in methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) isolates from animals. A total of 400 nasal swab samples were collected from cattle, goats and sheep between February and August 2022 from both industrial and traditional livestock farms in West Azerbaijan province, Iran. Then, nasal swabs were cultured on mannitol salt agar and molecular analysis was performed after bacteriological examination to confirm the presence of <i>S. aureus</i>. The <i>MecA</i> gene was used to detect MRSA isolates, and two important vancomycin resistance-associated genes, namely <i>vanA</i> and <i>vanB</i>, were searched in the isolates. Out of 400 nasal swabs, 69 samples had <i>S. aureus</i>; of which seven isolates were resistant against methicillin. No vancomycin resistance-associated genes were detected in the MRSA isolates. Based on these findings, vancomycin could be used to treat infections caused by this bacterium<i>.</i></p>\",\"PeriodicalId\":23989,\"journal\":{\"name\":\"Veterinary Research Forum\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11102584/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary Research Forum\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.30466/vrf.2023.2003964.3902\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/3/15 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Research Forum","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.30466/vrf.2023.2003964.3902","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/15 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ZOOLOGY","Score":null,"Total":0}
Screening of vancomycin resistance-associated genes in methicillin-resistant Staphylococcus aureus isolates from cattle, sheep and goats in northwestern Iran.
Staphylococcus aureus is an important pathogen causing a wide range of diseases in both humans and animals. The aim of this research was to screen the vancomycin resistance-associated genes in methicillin-resistant Staphylococcus aureus (MRSA) isolates from animals. A total of 400 nasal swab samples were collected from cattle, goats and sheep between February and August 2022 from both industrial and traditional livestock farms in West Azerbaijan province, Iran. Then, nasal swabs were cultured on mannitol salt agar and molecular analysis was performed after bacteriological examination to confirm the presence of S. aureus. The MecA gene was used to detect MRSA isolates, and two important vancomycin resistance-associated genes, namely vanA and vanB, were searched in the isolates. Out of 400 nasal swabs, 69 samples had S. aureus; of which seven isolates were resistant against methicillin. No vancomycin resistance-associated genes were detected in the MRSA isolates. Based on these findings, vancomycin could be used to treat infections caused by this bacterium.
期刊介绍：
Veterinary Research Forum (VRF) is a quarterly international journal committed to publish worldwide contributions on all aspects of veterinary science and medicine, including anatomy and histology, physiology and pharmacology, anatomic and clinical pathology, parasitology, microbiology, immunology and epidemiology, food hygiene, poultry science, fish and aquaculture, anesthesia and surgery, large and small animal internal medicine, large and small animal reproduction, biotechnology and diagnostic imaging of domestic, companion and farm animals.
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